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公开(公告)号：WO2010138182A3
公开(公告)日：2010-12-02
申请号：PCT/US2010/001543
申请日：2010-05-27
Applicant: LIFE TECHNOLOGIES CORPORATION , ROTHBERG, Jonathan M. , HINZ, Wolfgang , DAVIDSON, John F. , VAN OIJEN, Antoine M. , LEAMON, John H. , HUBER, Martin , BUSTILLO, James M. , MILGREW, Mark, James , SCHULTZ, Jonathan , MARRAN, David , REARICK, Todd , JOHNSON, Kim L.
Inventor： ROTHBERG, Jonathan M. , HINZ, Wolfgang , DAVIDSON, John F. , VAN OIJEN, Antoine M. , LEAMON, John H. , HUBER, Martin , BUSTILLO, James M. , MILGREW, Mark, James , SCHULTZ, Jonathan , MARRAN, David , REARICK, Todd , JOHNSON, Kim L.
Abstract: Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by- synthesis reactions. Some methods provided herein relate to improving signal (and also signal-to-noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
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公开(公告)号：EP4220146A1
公开(公告)日：2023-08-02
申请号：EP22188998.3
申请日：2010-05-27
Applicant: Life Technologies Corporation
Inventor： ROTHBERG, Jonathan M. , HINZ, Wolfgang , DAVIDSON, John F. , VAN OIJEN, Antoine M , LEAMON, John H. , HUBER, Martin , BUSTILLO, James M. , MILGREW, Mark, James , SCHULTZ, Jonathan , MARRAN, David , REARICK, Todd , JOHNSON, Kim L.
IPC: G01N27/416 , C12M1/34 , G01N27/414 , C12Q1/00
Abstract: An apparatus may include an array of sensors, a plurality of microwells, a row addressing circuit, a column addressing circuit, and analog-to-digital converters. Each sensor includes a two-transistor circuit with a chemically-sensitive field effect transistor (chemFET) and a row select transistor and each chemFETs has a floating gate structure and a passivation material located over at least a subset of the floating gate structures. The microwells define a plurality of cavities disposed over at least a portion of the floating gate structures and are of a size and shape capable of retaining analyte therein. The column addressing circuit is suitable for supplying output from each sensor in the array of sensors. The analog-to-digital converters are used for each column in the sensor array to convert an analog output of the column addressing circuit to digital form.
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公开(公告)号：EP2435128A2
公开(公告)日：2012-04-04
申请号：EP10780930.3
申请日：2010-05-27
Applicant: Life Technologies Corporation
Inventor： ROTHBERG, Jonathan M. , HINZ, Wolfgang , DAVIDSON, John F. , VAN OIJEN, Antoine M. , LEAMON, John H. , HUBER, Martin , BUSTILLO, James M. , MILGREW, Mark, James , SCHULTZ, Jonathan , MARRAN, David , REARICK, Todd , JOHNSON, Kim L.
CPC classification number: G01N27/4145 , C12Q1/6869 , C12Q1/6874 , G01N27/27 , G01N27/414 , Y10T29/49002
Abstract: Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
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Patent Agency Ranking4.发明公开Methods and apparatus for measuring analytes using large scale FET arrays 有权大规模方法和装置的分析物测量通过FET阵列的方法
公开(公告)号：EP2762872A1
公开(公告)日：2014-08-06
申请号：EP14152861.2
申请日：2007-12-14
Applicant: Life Technologies Corporation
Inventor： ROTHBERG, Jonathan M. , HINZ, Wolfgang , JOHNSON, Kim L. , BUSTILLO, James
IPC: G01N27/414 , G01N33/543
CPC classification number: G01N27/4148 , C12Q1/6818 , C12Q1/6874 , G01N27/4145 , G01N33/54373 , G01N33/5438 , H01L27/088 , H01L29/42324 , H01L29/78 , H01L2924/01006 , H01L2924/01013 , H01L2924/01015 , H01L2924/01073 , H01L2924/14 , H01L2924/1433 , Y10T436/22 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
Abstract: A circuit may include an array of CMOS-fabricated pixels formed in columns and rows. Each pixel (105) includes: a chemically-sensitive field effect transistor (chemFET) (150), a first switch (Q3), and a second switch (Q2). The chemFET has a floating gate structure, a source terminal, and a drain terminal that is coupled to a first readout signal line (116). The first switch couples the source terminal of the chemFET to a current source (106) and has one first transistor. The second switch couples the source terminal of the chemFET to a second readout signal line (114) and has one second transistor. The first and second switches are controlled by a row selection line (118) coupled to the two switches.
Abstract translation: 一种电路可以包括在制造形成在列和行的CMOS像素阵列。 各像素（105）包括：化学敏感的场效应晶体管（个chemFET）（150），第一开关（Q3），和第二开关（Q2）。 所述chemFET具有浮动栅极结构，源极端子和漏极端子也被连接到第一读出信号线（116）。 第一开关耦合在chemFET的源极端子到电流源（106），并具有一个第一晶体管。 所述第二开关耦合在chemFET的源极端子到第二读出信号线（114）和具有一个第二晶体管。 第一和第二开关由耦合到两个开关的行选择线（118）控制。
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5.发明授权Methods and apparatus for measuring analytes using large scale FET arrays 有权大规模方法和装置的分析物测量通过FET阵列的方法
公开(公告)号：EP2762872B1
公开(公告)日：2015-09-16
申请号：EP14152861.2
申请日：2007-12-14
Applicant: Life Technologies Corporation
Inventor： ROTHBERG, Jonathan M. , HINZ, Wolfgang , JOHNSON, Kim L. , BUSTILLO, James
IPC: G01N27/414 , G01N33/543
CPC classification number: G01N27/4148 , C12Q1/6818 , C12Q1/6874 , G01N27/4145 , G01N33/54373 , G01N33/5438 , H01L27/088 , H01L29/42324 , H01L29/78 , H01L2924/01006 , H01L2924/01013 , H01L2924/01015 , H01L2924/01073 , H01L2924/14 , H01L2924/1433 , Y10T436/22 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
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6.发明公开SCAFFOLDED NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING 有权NUCLEIC STAND系聚合物粒子及其制造方法和使用
公开(公告)号：EP2435461A1
公开(公告)日：2012-04-04
申请号：EP10780934.5
申请日：2010-05-27
Applicant: Life Technologies Corporation
Inventor： HINZ, Wolfgang , LEAMON, John H. , LIGHT, David , ROTHBERG, Jonathan M.
CPC classification number: C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6853 , C12Q2535/122 , C12Q2563/155 , C12Q2563/159 , C12Q2565/607 , C12Q2565/537 , C12Q2565/629
Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.
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公开(公告)号：EP2435461B1
公开(公告)日：2017-08-09
申请号：EP10780934.5
申请日：2010-05-27
Applicant: Life Technologies Corporation
Inventor： HINZ, Wolfgang , LEAMON, John H. , LIGHT, David , ROTHBERG, Jonathan M.
CPC classification number: C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6853 , C12Q2535/122 , C12Q2563/155 , C12Q2563/159 , C12Q2565/607 , C12Q2565/537 , C12Q2565/629
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8.发明授权
公开(公告)号：EP2092322B1
公开(公告)日：2016-02-17
申请号：EP07867780.4
申请日：2007-12-14
Applicant: Life Technologies Corporation
Inventor： ROTHBERG, Jonathan M. , HINZ, Wolfgang , JOHNSON, Kim L. , BUSTILLO, James
IPC: G01N27/414 , G01N33/543
CPC classification number: G01N27/4148 , C12Q1/6818 , C12Q1/6874 , G01N27/4145 , G01N33/54373 , G01N33/5438 , H01L27/088 , H01L29/42324 , H01L29/78 , H01L2924/01006 , H01L2924/01013 , H01L2924/01015 , H01L2924/01073 , H01L2924/14 , H01L2924/1433 , Y10T436/22 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
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