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    Specific Binding Assay Method With a Prosthetic Group as a Label Component
    23.
    发明专利
    Specific Binding Assay Method With a Prosthetic Group as a Label Component 未知

    公开(公告)号：GB2023607A

    公开(公告)日：1980-01-03

    申请号：GB7921693

    申请日：1979-06-21

    Applicant: MILES LAB

    Inventor： HORNBY WILLIAM E ,  MORRIS DAVID L

    IPC: G01N33/532 ,  C12Q1/00 ,  C12Q1/26 ,  G01N33/536 ,  G01N33/542 ,  C07H19/20 ,  G01N31/14

    Abstract: A specific binding assay for determining a ligand in a liquid medium employing an organic prosthetic group residue, such as a residue of flavin adenine dinucleotide, flavin mononucleotide, or heme, as a label component in the labeled conjugate, Preferably, the label component is the prosthetic group residue alone or is a holoenzyme residue comprising such prosthetic group residue combined with an apoenzyme in the form of a holoenzyme complex. In the former case, the label component preferably is monitored in the assay by adding an apoenzyme after the binding reaction has been initiated and measuring the resultant holoenzyme activity. In the latter case, the label component is monitored simply by measuring holoenzyme activity. The assay method may follow conventional homogeneous and heterogeneous schemes. Preferred apoenzymes for use in the assay are apoglucose oxidase and apoperoxidase. The assay offers the advantages of colorimetric read-out and of being readily adaptable to automated techniques. Also disclosed are prosthetic group- labeled and enzyme-labeled conjugates for use in the assay, as well as a method for preparing the latter conjugates.

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