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    IODOTHYRONINE IMMUNOASSAYS EMPLOYING HMS AS TBP BLOCKING AGENT
    5.
    发明专利
    IODOTHYRONINE IMMUNOASSAYS EMPLOYING HMS AS TBP BLOCKING AGENT 未知

    公开(公告)号：CA1225024A

    公开(公告)日：1987-08-04

    申请号：CA451292

    申请日：1984-04-04

    Applicant: MILES LAB

    Inventor： ELLIS PAUL B ,  MORRIS DAVID L

    IPC: G01N33/537 ,  G01N33/53 ,  G01N33/535 ,  G01N33/542 ,  G01N33/78 ,  G01N33/566

    Abstract: An improved immunoassay method, reagent means, test kit, and test device for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or plasma, wherein 2-hydroxy-4-methoxy-benzophenone-5-sulfonic acid (HMS), or a salt thereof, is employed as a blocking agent for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays employing labels which are participants in enzyme-catalyzed reactions. Such labels include enzyme substrates, coenzymes, enzyme inhibitors, enzyme prosthetic groups, and enzymes.

    ACTIVATED APOGLUCOSE OXIDASE AND ITS USE IN SPECIFIC BINDING ASSAYS
    8.
    发明专利
    ACTIVATED APOGLUCOSE OXIDASE AND ITS USE IN SPECIFIC BINDING ASSAYS 未知

    公开(公告)号：CA1172960A

    公开(公告)日：1984-08-21

    申请号：CA396257

    申请日：1982-02-15

    Applicant: MILES LAB

    Inventor： MORRIS DAVID L

    IPC: C12Q1/26 ,  G01N33/54

    Abstract: A method for increasing the ability of apoglucose oxidase to combine with flavin adenine dinucleotide (FAD) and derivatives thereof to form active glucose oxidase by interacting apoglucose oxidase with an immunologically derived binding substance, e.g., an antibody or a fragment thereof, having a specific binding affinity for glucose oxidase. The apoglucose oxidase/anti-glucose oxidase immune complex is characterized by enhanced ability to combine with FAD and FAD-derivatives to yield glucose oxidase activity. The activation is particularly significant at temperatures elevated from room temperature, e.g., between 30.degree.-45.degree.C. An improved homogeneous specific binding assay method is provided for determining ligands wherein an FAD label is used and is monitored by its ability to combine with apoglucose oxidase to form active glucose oxidase by including anti-glucose oxidase in the reaction mixture.

    10.
    发明专利
    未知

    公开(公告)号：NO823658L

    公开(公告)日：1983-05-05

    申请号：NO823658

    申请日：1982-11-03

    Applicant: MILES LAB

    Inventor： ATKINSON DAVID C ,  CARRICO ROBERT JOSEPF ,  MORRIS DAVID L

    IPC: G01N33/78 ,  G01N

    Abstract: An improved immunoassay method, reagent means, and test kit for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or piasma, wherein fenclofenac and related phenylacetic acids, or salts thereof, are employed as novel blocking agents for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays wherein a spectrophotometric response is generated in the assay reaction mixture at a wavelength greater than about 300 nm, the blocking agents of the present invention having been found to have no substantial absorbance at wavelengths above 300 nm. Such homogeneous immunoassays include those which employ labels such as fluorescers, enzyme substrates, enzyme prosthetic groups, enzymes, and enzyme inhibitors.

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