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    SPECIFIC BINDING ASSAY WITH A PROSTHETIC GROUP AS A LABEL COMPONENT
    24.
    发明专利
    SPECIFIC BINDING ASSAY WITH A PROSTHETIC GROUP AS A LABEL COMPONENT 未知

    公开(公告)号：ZA7903068B

    公开(公告)日：1980-08-27

    申请号：ZA7903068

    申请日：1979-06-20

    Applicant: MILES LAB

    Inventor： MORRIS DAVID L ,  HORNBY WILLIAM E

    IPC: G01N33/532 ,  C12Q1/00 ,  C12Q1/26 ,  G01N33/536 ,  G01N33/542 ,  C07H ,  G01N

    CPC classification number: G01N33/542

    Abstract: A specific binding assay for determining a ligand in a liquid medium employing an organic prosthetic group residue, such as a residue of flavin adenine dinucleotide, flavin mononucleotide, or heme, as a label component in the labeled conjugate, Preferably, the label component is the prosthetic group residue alone or is a holoenzyme residue comprising such prosthetic group residue combined with an apoenzyme in the form of a holoenzyme complex. In the former case, the label component preferably is monitored in the assay by adding an apoenzyme after the binding reaction has been initiated and measuring the resultant holoenzyme activity. In the latter case, the label component is monitored simply by measuring holoenzyme activity. The assay method may follow conventional homogeneous and heterogeneous schemes. Preferred apoenzymes for use in the assay are apoglucose oxidase and apoperoxidase. The assay offers the advantages of colorimetric read-out and of being readily adaptable to automated techniques. Also disclosed are prosthetic group- labeled and enzyme-labeled conjugates for use in the assay, as well as a method for preparing the latter conjugates.

    UN METODO DE ENSAYO DE FIJACION ESPECIFICO PARA DETERMINAR UN LIGANDO EN UN MEDIO LIQUIDO.
    25.
    发明专利
    UN METODO DE ENSAYO DE FIJACION ESPECIFICO PARA DETERMINAR UN LIGANDO EN UN MEDIO LIQUIDO. 未知

    公开(公告)号：ES481782A1

    公开(公告)日：1980-07-01

    申请号：ES481782

    申请日：1979-06-21

    Applicant: MILES LAB

    Inventor： HORNBY WILLIAM E ,  MORRIS DAVID L

    IPC: G01N33/532 ,  C12Q1/00 ,  C12Q1/26 ,  G01N33/536 ,  G01N33/542 ,  G01N31/14

    Abstract: Ensayo de fijación específico mejorado por el empleo de un resto de grupo prostético orgánico como componente de marcado en el conjugado de marcado. Los grupos prostéticos son una clase de cofactores de enzimas reconocida en la técnica. Un cofactor de enzima es una sustancia no proteínica cuya presencia se requiere para que una enzima exhiba su actividad catalítica y que sufre un cambio químico durante el ciclo catalítico de la enzima de que se trate (a la que se hace referencia como la enzima madre). Una coenzima es un tipo de cofactor de enzima que se modifica químicamente en el curso de la reacción catalizada por la enzima madre. La regeneración de la forma original del cofactor requiere su participación en una reacción separada que es catalizada por una enzima diferente en la enzima madre.

    28.
    发明专利
    未知

    公开(公告)号：NO792084A

    公开(公告)日：1979-12-28

    申请号：NO792084

    申请日：1979-06-21

    Applicant: MILES LAB

    Inventor： HORNBY WILLIAM E ,  MORRIS DAVID L

    IPC: G01N33/532 ,  C12Q1/00 ,  C12Q1/26 ,  G01N33/536 ,  G01N33/542 ,  G01N

    CPC classification number: G01N33/542

    Abstract: A specific binding assay for determining a ligand in a liquid medium employing an organic prosthetic group residue, such as a residue of flavin adenine dinucleotide, flavin mononucleotide, or heme, as a label component in the labeled conjugate, Preferably, the label component is the prosthetic group residue alone or is a holoenzyme residue comprising such prosthetic group residue combined with an apoenzyme in the form of a holoenzyme complex. In the former case, the label component preferably is monitored in the assay by adding an apoenzyme after the binding reaction has been initiated and measuring the resultant holoenzyme activity. In the latter case, the label component is monitored simply by measuring holoenzyme activity. The assay method may follow conventional homogeneous and heterogeneous schemes. Preferred apoenzymes for use in the assay are apoglucose oxidase and apoperoxidase. The assay offers the advantages of colorimetric read-out and of being readily adaptable to automated techniques. Also disclosed are prosthetic group- labeled and enzyme-labeled conjugates for use in the assay, as well as a method for preparing the latter conjugates.

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