公开(公告)号：JP2008043244A

公开(公告)日：2008-02-28

申请号：JP2006221109

申请日：2006-08-14

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  MATSUO YUICHIRO ,  SHIMADA YOSHIHIRO

IPC: C12M1/34 ,  C12Q1/02 ,  G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6458 ,  G01N2021/6482

Abstract: PROBLEM TO BE SOLVED: To acquire the clear image of small-diameter projection part and to improve an analysis accuracy without using a complicated dyeing method.
SOLUTION: The apparatus 1 for automatic cell analysis is equipped with an imaging device 4 for imaging fluorescence emitted from cells S and acquiring cell images, an exposure change means 5 for changing exposure conditions in the acquisition of cell images by the imaging device 4 and a treatment means 5 for analyzing the cells S on the basis of each of a plurality of acquired cell images under the changed exposure conditions.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：为了获得小直径投影部分的清晰图像，并且在不使用复杂的染色方法的情况下提高分析精度。 解决方案：用于自动细胞分析的装置1配备有用于成像装置4，用于对从小区S发射的荧光进行成像并获取细胞图像;曝光改变装置5，用于改变成像装置获取细胞图像中的曝光条件 以及处理装置5，用于基于改变的曝光条件下的多个获取的细胞图像中的每一个来分析细胞S. 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2019090892A

公开(公告)日：2019-06-13

申请号：JP2017218710

申请日：2017-11-14

Applicant: OLYMPUS CORP

Inventor： SHIMADA YOSHIHIRO ,  GODA KAZUHITO

IPC: G02B21/00 ,  G02B21/34

Abstract: 【課題】顕微鏡が本来有する光学性能を最大限発揮でき、顕微鏡による細胞凝集塊の高精細な観察および撮像が可能であるとともに、観察の自動化を容易にする。【解決手段】少なくとも1つの細胞凝集塊Sを内包したゲル状の透明なハンギングドロップDとウエル3の透明な底部3aとを接触させ、その接触領域が顕微鏡の対物レンズ9の有効光束が通過する大きさになるまで、ハンギングドロップDと底部3aとの間に相対的に近接する方向に押圧力を作用させた状態で、細胞凝集塊Sからの光をウエル3を介して対物レンズ9により集光して細胞凝集塊Sを観察する試料観察方法を提供する。【選択図】図2

公开(公告)号：JP2010252648A

公开(公告)日：2010-11-11

申请号：JP2009104026

申请日：2009-04-22

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： YAMAGUCHI MITSUSHIRO ,  SHIMADA YOSHIHIRO

IPC: C12Q1/02 ,  G01N21/64 ,  G01N21/78 ,  G01N33/48 ,  G01N33/483

Abstract: PROBLEM TO BE SOLVED: To provide a method for analyzing cells by which the throughput of the analysis is improved by shortening an imaging time, and a management cost of data can be reduced by reducing the amount of the data.
SOLUTION: The method for analyzing the cells by taking the image of a cell group, recognizing each of the cells by recognizing a first component in each cell by using the taken image, and evaluating characteristic amounts of components except the first component by the cell includes the step of labeling the first component with a first dye, the step of labeling the components except the first component with a second dye, the step of simultaneously taking the image of the first component and the components except the first component by changing the brightness level of the light from a first dye from the brightness level of the light from the second dye in a degree of enabling a threshold value to be set, the step of recognizing the first component and the components except the first component from the obtained image via the brightness threshold value, and the evaluation step of evaluating by the cell the characteristic amounts of the components except the first component by evaluating the characteristics of the light from the second dye in the region of the components except the first component in the obtained image.
COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 要解决的问题：提供一种分析小区的方法，通过缩短成像时间来提高分析的吞吐量，并且可以通过减少数据量来减少数据的管理成本。 解决方案：通过拍摄单元组的图像来分析单元的方法，通过使用拍摄图像识别每个单元中的第一分量来识别每个单元，并且通过以下方式评估除第一分量之外的分量的特征量： 单元包括用第一染料标记第一组分的步骤，用第二染料标记除了第一组分之外的组分的步骤，通过改变第一组分和除第一组分之外的组分同时获取图像的步骤 来自第一染料的光的亮度水平以能够设定阈值的程度来自第二染料的光的亮度水平，从所获得的步骤识别第一成分和除第一成分以外的成分 通过亮度阈值进行图像的评价，以及评价步骤，通过评价来评价除了第一成分以外的成分的特征量 g所得图像中除了第一成分以外的成分区域中的第二染料的光的特性。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2010216919A

公开(公告)日：2010-09-30

申请号：JP2009062607

申请日：2009-03-16

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TSUTSUI AKIRA ,  MATSUO YUICHIRO ,  SHIMADA YOSHIHIRO

IPC: G01N21/17 ,  C12M1/34 ,  G01N21/64

Abstract: PROBLEM TO BE SOLVED: To provide an image pickup device for certainly extracting a target signal from all of imaging target regions at a high speed to perform detailed analysis, not becoming large-scale and for being reduced in cost, and a cell image analyzing system equipped with the same. SOLUTION: The electron multiplying gain of a cooling CCD camera 4 is made high, the resolving power of the cooling CCD camera is made low and the exposure time of a light source 3 is made short with respect to all of the imaging target regions in a container 1 to perform imaging in a high throughput mode and the target signal is extracted from an image group picked up in the high throughput mode on the basis of a predetermined extraction condition. Subsequently, the electron multiplying gain of the cooling CCD camera is made low, the resolving power of the cooling CCD camera is made high and the exposure time of the light source is made long only with respect to the imaging target regions corresponding to the region where the target signal in the image, wherein the target signal is extracted in the container, to perform imaging in a highly detailed mode. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供一种用于从所有成像目标区域高速提取目标信号以进行详细分析，不会变得大规模并且降低成本的图像拾取装置和单元 图像分析系统配备相同。 解决方案：使冷却CCD摄像机4的电子倍增增益变高，冷却CCD摄像机的分辨率降低，光源3的曝光时间相对于所有成像目标变短 容器1中的区域，以高通量模式进行成像，并且基于预定的提取条件从在高通量模式中拾取的图像组中提取目标信号。 随后，冷却CCD摄像机的电子倍增增益变低，使得冷却CCD摄像机的分辨率高，并且光源的曝光时间只对相对于与 图像中的目标信号，其中在容器中提取目标信号，以高度详细的模式进行成像。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2009003094A

公开(公告)日：2009-01-08

申请号：JP2007162591

申请日：2007-06-20

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： HARADA MITSUO ,  SHIMADA YOSHIHIRO

IPC: G02B21/24 ,  G01N21/64

CPC classification number: G01N21/6458 ,  G02B21/0088 ,  G02B21/24 ,  G02B21/362

Abstract: PROBLEM TO BE SOLVED: To provide a box type microscopic apparatus capable of easily placing a sample container at an optimum position without adjusting the position of the sample container on a stage when changing the sample container with inexpensive and simple constitution, and easily and rapidly changing the sample container.
SOLUTION: The box type microscopic apparatus which includes the stage 12, a microscope and a housing 20, and where the housing 20 is equipped with a fixed housing 21 and a movable housing 22 capable of moving to be openable/closable relative to the fixed housing 21 includes: a sample container positioning means 50 fixing the sample container 40 placed on the stage 12 at the fixed position on the stage 12; and a positioning release means 60 for actuating the sample container positioning means 50 when the movable housing 22 moves in a closing direction, and releases the positioning of the sample container 40 relative to the stage 12 by the sample container positioning means 50 when the movable housing 22 is opened.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供一种盒式微型设备，其能够以便宜且简单的结构更换样品容器时，容易地将样品容器放置在最佳位置，而不需要在样品容器的台架上调整位置，并且容易 并快速更换样品容器。 解决方案：包括平台12，显微镜和外壳20的盒型显微镜装置，并且其中壳体20装备有固定壳体21和能够相对于相对于壳体20可打开/关闭的可移动壳体22移动 固定壳体21包括：样品容器定位装置50，其将放置在载物台12上的样品容器40固定在载物台12上的固定位置; 以及定位释放装置60，用于当可移动壳体22沿关闭方向移动时致动样本容器定位装置50，并且当可移动外壳（10）移动时，通过样本容器定位装置50释放样本容器40相对于平台12的定位 22打开。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2008046305A

公开(公告)日：2008-02-28

申请号：JP2006221108

申请日：2006-08-14

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  MATSUO YUICHIRO ,  SHIMADA YOSHIHIRO

IPC: G02B7/28 ,  G02B7/36 ,  G02B21/00 ,  H04N5/232

CPC classification number: G02B21/244 ,  G01N21/6458 ,  H04N5/23212

Abstract: PROBLEM TO BE SOLVED: To provide an automatic focusing device where, in the acquisition of the image of a cell, a focal position is correctly set to a cell having high activity without adjusting the focus on a dead cell.
SOLUTION: The automatic focusing device 8 used for a microscope 1 where fluorescence emitted from a cell is imaged to acquire a cell image, is provided with: a setting means 5 for setting a luminance range showing the presence region of a living cell on the basis of the luminance distribution of the acquired cell image; and a focusing detection means 5 for detecting the focal position on the basis of luminance of the image of the nuclei of the cell in the luminance range set by the setting means 5.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供一种自动对焦装置，其中，在获取单元的图像时，将焦点位置正确地设置为具有高活动性的单元，而不调整对死细胞的聚焦。 解决方案：用于显微镜1的自动聚焦装置8，其中从单元发射的荧光成像以获取细胞图像，设置有：设定装置5，用于设定表示活细胞存在区域的亮度范围 基于所获取的小区图像的亮度分布; 以及聚焦检测装置5，用于基于由设置装置5设置的亮度范围中的单元的核的图像的亮度来检测焦点位置。（C）2008年，JPO和INPIT

公开(公告)号：JP2007020449A

公开(公告)日：2007-02-01

申请号：JP2005205574

申请日：2005-07-14

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  SHIMADA YOSHIHIRO ,  MATSUO YUICHIRO ,  KAWAI TAKESHI

IPC: C12Q1/02 ,  C12M1/34 ,  G01N33/15 ,  G01N33/50 ,  G06T1/00

Abstract: PROBLEM TO BE SOLVED: To carry out the screening of medicines in high accuracy by using a proper morphological character.
SOLUTION: The screening method comprises the analysis of cell images photographed by a microscope, the evaluation of the parameterized value of the deviation of the contour of the cell from circles C
I and C
O as a morphological character representing the morphological character of each cell in the cell images and the screening of medicines based on the evaluation.
COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 要解决的问题：通过适当的形态特征，高精度地进行药物筛选。 解决方案：筛选方法包括通过显微镜拍摄的细胞图像的分析，细胞轮廓偏离参数值的评估圆圈C I 和C O 作为代表细胞图像中的每个细胞的形态特征和基于评估的药物筛选的形态特征。 版权所有（C）2007，JPO＆INPIT

公开(公告)号：JP2005227097A

公开(公告)日：2005-08-25

申请号：JP2004035341

申请日：2004-02-12

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  SHIMADA YOSHIHIRO

IPC: G01N21/64 ,  C12M1/00 ,  G01N33/48 ,  G01N33/483

Abstract: PROBLEM TO BE SOLVED: To provide a cell image analyzer capable of precisely extracting the total number of cells or the characteristic quantity of an individual cell. SOLUTION: The cell image analyzer is equipped with a cell image acquiring means 55 for imaging a plurality of cells developed in color by different dyes under different conditions to acquire a plurality of images and an image analyzing means 80 for extracting predetermined element contained in the respective cells in a plurality of images. COPYRIGHT: (C)2005,JPO&NCIPI

Abstract translation: 要解决的问题：提供一种能够精确提取单元的总数或特征量的单元图像分析装置。 解决方案：细胞图像分析仪装备有细胞图像获取装置55，用于在不同条件下对通过不同染料显色的多个细胞进行成像以获取多个图像;以及图像分析装置80，用于提取含有 在多个图像中的各个单元中。 版权所有（C）2005，JPO＆NCIPI

公开(公告)号：JP2011118435A

公开(公告)日：2011-06-16

申请号：JP2011055823

申请日：2011-03-14

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： SHIMADA YOSHIHIRO

IPC: G02B21/24

CPC classification number: G02B21/088 ,  G02B21/16

Abstract: PROBLEM TO BE SOLVED: To reduce the size and production costs of an optical microscope blocking light or maintaining the specimen environment. SOLUTION: The provided optical microscope apparatus 1 includes: a microscope 2 that has a stage 4 for mounting a specimen A, a transmission-illumination optical system 6, and an image-forming optical system 7; and a housing 3 that surrounds the microscope 2, wherein the housing 3 includes a fixed housing 24, and a movable housing 25. Among optical parts constituting the transmission-illumination optical system 6 and the image-forming optical system 7, at least some optical parts disposed above the stage 4 are movable, and a switching mechanism is provided which is configured to retract the optical parts away from above the stage 4 when the movable housing 25 is disposed in an open position relative to the fixed housing 24, and to substantially align the optical axes of both optical systems when the movable housing 25 is disposed in a closed position relative to the fixed housing 24. COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 要解决的问题：减少光学显微镜遮挡光线或维持样品环境的尺寸和生产成本。 解决方案：提供的光学显微镜装置1包括：具有用于安装试样A的载物台4，透射照明光学系统6和成像光学系统7的显微镜2; 以及围绕显微镜2的壳体3，其中壳体3包括固定壳体24和可移动壳体25.在构成透射照明光学系统6和图像形成光学系统7的光学部件中，至少一些光学 设置在平台4上方的部件是可移动的，并且设置有切换机构，该切换机构构造成当可移动壳体25相对于固定壳体24设置在打开位置时将光学部件从工作台4上方缩回， 当可动壳体25相对于固定壳体24设置在关闭位置时，对准两个光学系统的光轴。版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2010060790A

公开(公告)日：2010-03-18

申请号：JP2008225727

申请日：2008-09-03

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： SHIMADA YOSHIHIRO

IPC: G02B21/24 ,  G01N21/64 ,  G01N21/78

Abstract: PROBLEM TO BE SOLVED: To provide an optical microscope that prevents entry of excitation light, harmful to the eyes of an operator, by an optical attenuation member, that allows the operator to confirm the position of a dispensing burette and to perform safe manual dispensing, and allows the operator, regardless of whether he or she is right- or left-handed, to carry out manual dispensing by area provided on the side of the optical attenuation member, thereby facilitating dispensing.
SOLUTION: The optical microscope includes: a housing for shielding light or maintaining environment for a specimen; and a door 4 that is provided in the housing 1 and can be opened and closed for allowing the operator to perform manual dispensing on a specimen container enclosing a living-organism sample, which is an observation subject. In the optical microscope, a part of an aperture 2 defined when the door is opened is covered by the optical attenuation member 4 that shields light of a specific wavelength.
COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供一种光学显微镜，其防止通过光学衰减构件进入对操作者的眼睛有害的激发光，从而允许操作者确认分配滴定管的位置并执行安全 手动分配，并且允许操作者，无论他或她是右还是左手，通过设置在光衰减构件一侧的区域进行手动分配，从而便于分配。 解决方案：光学显微镜包括：用于屏蔽光线或维持样品环境的外壳; 以及设置在壳体1中并能够打开和关闭以允许操作者在包围作为观察对象的生物体样本的标本容器上进行手动分配的门4。 在光学显微镜中，当门打开时限定的孔2的一部分被屏蔽特定波长的光的光学衰减构件4覆盖。 版权所有（C）2010，JPO＆INPIT