公开(公告)号：US09103718B2

公开(公告)日：2015-08-11

申请号：US13888868

申请日：2013-05-07

Applicant: OLYMPUS CORPORATION

Inventor： Takuya Hanashi ,  Tetsuya Tanabe ,  Mitsushiro Yamaguchi

IPC: G01N21/00 ,  G01N21/17 ,  G01J1/16 ,  G01N21/64 ,  G01N15/14 ,  G02B21/00 ,  G01J1/58 ,  G01C21/02 ,  G02B21/26

CPC classification number: G01J1/16 ,  G01C21/02 ,  G01J1/58 ,  G01N15/1463 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6421 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/26

Abstract: The inventive technique of detecting and analyzing light from a light-emitting particle in accordance with the scanning molecule counting method using an optical measurement with a confocal microscope or a multiphoton microscope is characterized by detecting intensities of components of two or more wavelength bands of light from a light detection region of an optical system with moving the position of the light detection region in a sample solution by changing the optical path of the optical system of the microscope; detecting individually signals of the light from each light-emitting particle in the intensities of the components of the two or more wavelength bands of the detected light; and identifying a kind of light-emitting particle based on the intensities of the components of the two or more wavelength bands of the signals of the light of the detected light-emitting particle.

Abstract translation: 根据使用共聚焦显微镜或多光子显微镜的光学测量的扫描分子计数法检测和分析来自发光粒子的光的本发明的技术的特征在于检测两个或更多个波长带的光的分量的强度， 光学系统的光检测区域，通过改变显微镜的光学系统的光路来移动样品溶液中的光检测区域的位置; 在检测到的光的两个或更多个波长带的分量的强度中单独地检测来自每个发光粒子的光的信号; 并且基于检测到的发光粒子的光的信号的两个或更多波长带的分量的强度来识别发光粒子的种类。

公开(公告)号：US20150108369A1

公开(公告)日：2015-04-23

申请号：US14496177

申请日：2014-09-25

Applicant: OLYMPUS CORPORATION

Inventor： Takuya Hanashi

IPC: G01N21/64 ,  G01N15/14

CPC classification number: G01N21/64 ,  G01N15/1429 ,  G01N15/1434 ,  G01N21/6458 ,  G01N2015/1486 ,  G01N2201/06113 ,  G02B21/0064 ,  G02B21/0076 ,  G02B21/0084

Abstract: There is provided a single particle detection technique based on the scanning molecule counting method which individually detects single particles using light measurement with a confocal or multiphoton microscope, where the existences of a non-light-emitting particle and a light-emitting particle can be detected while being discriminated from one another in a sample solution. The inventive technique of detecting a single particle detects light from a light detection region during moving the position of the light detection region of the microscope in a sample solution containing a non-light-emitting particle and a light-emitting particle to generate time series light intensity data; and detects in the time series light intensity data a light intensity increase relative to background light intensity as a signal indicating the existence of the light-emitting particle and a light intensity reduction relative to the background light intensity as a signal indicating the existence of the non-light-emitting particle.

Abstract translation: 提供了基于扫描分子计数方法的单粒子检测技术，其使用共聚焦或多光子显微镜单独检测使用光测量的单个颗粒，其中可以检测到非发光颗粒和发光颗粒的存在 同时在样品溶液中彼此区分开来。 本发明的检测单个颗粒的技术在将显微镜的光检测区域的位置移动到含有非发光颗粒和发光颗粒的样品溶液中的同时，检测来自光检测区域的光，以产生时间序列光 强度数据; 并且以时间序列光强度数据检测作为指示发光粒子存在的信号的相对于背景光强度的光强度增加，并且相对于背景光强度的光强度降低为指示存在非光照强度的信号 发光粒子。

公开(公告)号：US20130122488A1

公开(公告)日：2013-05-16

申请号：US13746968

申请日：2013-01-22

Applicant: OLYMPUS CORPORATION

Inventor： Tetsuya Tanabe ,  Hidetaka Nakata ,  Takuya Hanashi ,  Kunio Hori ,  Kazutaka Nishikawa

IPC: G01N33/53 ,  C12Q1/68

CPC classification number: G01N33/5308 ,  C12Q1/6818 ,  G01N5/045 ,  G01N15/1456 ,  G01N21/6458 ,  G01N2015/1486 ,  G02B21/0048 ,  G02B21/0076

Abstract: There is provided an optical analysis technique enabling the detection of the condition or characteristic of a particle to be observed contained at a low concentration or number density in a sample solution using a light-emitting probe. The inventive optical analysis technique uses an optical system capable of detecting light from a micro region in a solution, such as an optical system of a confocal microscope or a multiphoton microscope, to detect the light from the light-emitting probe having bound to a particle to be observed while moving the position of the micro region in the sample solution (while scanning the inside of the sample solution with the micro region), thereby detecting individually the particle crossing the inside of the micro region to enable the counting of the particle(s) or the acquisition of the information on the concentration or number density of the particle.

Abstract translation: 提供了一种光学分析技术，其能够使用发光探针检测在样品溶液中以低浓度或数量密度包含的待观察颗粒的状态或特性。 本发明的光学分析技术使用能够检测来自诸如共焦显微镜或多光子显微镜的光学系统的解决方案中的微区域的光的光学系统，以检测来自发光探针的光，其结合到颗粒 在移动样品溶液中的微区域的位置（同时用微区域扫描样品溶液的内部）的同时观察，从而单独检测与微区域内部交叉的粒子，以使得能够计数颗粒（ s）或获取关于颗粒的浓度或数量密度的信息。