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公开(公告)号：JPS5480494A
公开(公告)日：1979-06-27
申请号：JP5436978
申请日：1978-05-10
Applicant: ORIENTAL YEAST CO LTD
Inventor： WATANABE TOSHIO , OKA OSAMU , HODA YASUKO , SAEKI TAKAMICHI
Abstract: PURPOSE:To prepare NAD from a precursor comprising quinolinic acid, economically and efficiently, by repeatedly using NAD-producing cells of yeast treated with a cationic surface active agent, as a bio-catalyst. CONSTITUTION:Cells of yeast obtained by culturing yeast belonging to Saccharomyces, Schizosaccharomyces, Zygosaccharomyces, or Candida genus, and capable of producing nicotinamide adenine dinucleotide, e.g. Saccharomyces cerevisiae which is readily available Baker's yeast, are treated with a cationic surface active agent such as cetyltrimethylammonium bromide (CTAB). NAD is prepared by reacting (A) quinolinic acid, (B) ribose-5-phosphoric acid and (C) adenosine-5-phosphoric acid, in the presence of the above treated cells in a tris-hydrochloric acid buffer solution containing Mg salt, pref. at pH of 2.5-7.5 and 25-40 deg.C for 0.5-6 hours.
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公开(公告)号：JP2001299361A
公开(公告)日：2001-10-30
申请号：JP2000130176
申请日：2000-04-28
Applicant: ORIENTAL YEAST CO LTD
Inventor： UCHIDA KOJI , MATSUKAWA HIROKAZU , KODA SEIICHI , OKA OSAMU , MATSUO TAKESHI
IPC: C12N15/09 , A61K38/00 , A61P9/00 , A61P9/06 , A61P9/10 , A61P35/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/12
Abstract: PROBLEM TO BE SOLVED: To obtain the subject heterodimer is obtained using a vector containing a nucleic acid encoding the M-type subunit of creatine kinase and a 2nd nucleic acid encoding the B-type subunit of the creatine kinase. SOLUTION: This creatine kinase heterodimer is characterized by being obtained using the vector.
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公开(公告)号：JPS5754850A
公开(公告)日：1982-04-01
申请号：JP12917980
申请日：1980-09-19
Applicant: Oriental Yeast Co Ltd
Inventor： UTSUKI YOSHIO , OOHASHI MINORU , OKA OSAMU
IPC: G01N27/416 , G01N31/00
CPC classification number: G01N31/00
Abstract: PURPOSE:To measure a small amount of H2O2 in the ppb order for a short time by adding a H2O2 decomposition agent solution thereto after dissolved oxygen in a test liquid is removed. CONSTITUTION:A polarograph electrode 1 is mounted on the side wall of a reaction vessel 3. 1ml of a test liquid is injected into the reactive vessel 3 and closed with a ceptum 4. Then, a nitrogen gas is supplied to the vessel 3 at a rate of 30ml/min from a supply tube 10. Dissolved oxygen in the test liquid is removed for 4-5min. Confirming the removal, the supply of the nitrogen gas is halted. The reaction vessel 3 is left closed, where the test liquid contains no dissolved oxygen. Then, the nitrogen gas is fed into an enzyme catalase solution as an H2O2 decomposition agent to remove oxygen and is sampled up to 2.0mu with a syrinze preventing redissolution of oxygen. It is injected into the reaction vessel 3 quickly through the captum 4. Perhydride in the test liquid is decomposed with the catalase and the increment of oxygen generated is detected with an oxygen electrode 1 for 1-2min.
Abstract translation: 目的：通过在溶液中除去溶解氧后，通过加入H2O2分解剂溶液，在短时间内以ppb顺序测量少量的H 2 O 2。 构成：将极谱法电极1安装在反应容器3的侧壁上。将1ml试验液注入反应容器3中，用塞子4封闭。然后，将氮气以 从供应管10的速度为30ml / min。将测试液中的溶解氧除去4-5分钟。 确认清除，停止供应氮气。 将反应容器3保持关闭，其中测试液体不含溶解氧。 然后，将氮气作为H 2 O 2分解剂加入酶过氧化氢酶溶液中以除去氧气，并用syrinze取样至2.0mu，防止氧气再溶解。 将其通过捕集剂4快速注入反应容器3中。测试液中的过氧化物用过氧化氢酶分解，用氧电极1检测产生的氧气增加1-2分钟。
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公开(公告)号：JPS54101491A
公开(公告)日：1979-08-10
申请号：JP617078
申请日：1978-01-25
Applicant: ORIENTAL YEAST CO LTD
Inventor： FUJITA TAKESHI , OKA OSAMU , KOUKAWARA ISAMU , HORIO BUICHI
Abstract: PURPOSE:To prepare a reduced NAD (nicotinamide adenine dinucleotide) using only a small amount of enzyme, in high yield, by reacting NAD with formic acid in the presence of formate-dehydrogenase while evacuating and removing the produced CO2 gas from the reaction system. CONSTITUTION:NAD is reacted with formic acid in a buffer solution having a pH of about 7.5, in the presence of formate-dehydrogenase at 20-50 deg.C for 1-2 hours under a reduced pressure, while removing the produced CO2 gas from the system. After the completion of the reaction, the enzyme is precipitated by adding an alcohol (vol. ratio of the reaction mixture to the alcohol is 1 to 2), and the filtrate is added with an alcohol until alcohol concentration reaches 90-93%. The objective reduced NAD can be obtained as precipitate in an yield of as high as 95%.
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公开(公告)号：JPS52110887A
公开(公告)日：1977-09-17
申请号：JP2604176
申请日：1976-03-12
Applicant: ORIENTAL YEAST CO LTD
Inventor： WATANABE TOSHIO , OKA OSAMU , HODA YASUKO , SAEKI KEIDOU
IPC: C12P19/32 , C12N1/16 , C12N11/16 , C12P1/00 , C12P19/36 , C12P21/02 , C12R1/645 , C12R1/72 , C12R1/865
Abstract: PURPOSE:In the biozynthesis of physiologically active substances by the use of dry yeast, the reaction rate and product yield can be enhanced by using yeast culture previously moistened under specific conditions.
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Patent Agency Ranking
公开(公告)号：JPS51141899A
公开(公告)日：1976-12-07
申请号：JP6600075
申请日：1975-06-03
Applicant: ORIENTAL YEAST CO LTD
Inventor： HODA YASUKO , WATANABE TOSHIO , OKA OSAMU , SAEKI KEIDOU
IPC: C07K5/037 , C07C231/00 , C07K14/415
Abstract: PURPOSE:To prepare a glutathione-containing solution at low cost with the inhibition of its decomposition by the water extraction from embryos as the staring raw materials with adjusting its pH condition.
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公开(公告)号：JP2014200183A
公开(公告)日：2014-10-27
申请号：JP2013077649
申请日：2013-04-03
Applicant: オリエンタル酵母工業株式会社 , Oriental Yeast Co Ltd
Inventor： URASAKI HIROMI , KODOI RIE , OKA OSAMU
Abstract: 【課題】赤飯として求められる色調を有し、風味に優れ、かつ、金属味及び収斂味が抑制された赤飯の素及びその製造方法、前記赤飯の素を使用した赤飯及びその製造方法、並びに、前記赤飯の素の製造に用いる赤飯の素製造用キットの提供。【解決手段】アズキ(Vignaangularis)及びササゲ(Vignaunguiculata)の少なくともいずれかを含有する豆含有組成物と、炭酸カリウム含有組成物と、鉄含有酵母抽出物含有組成物とを含有する混合液を加熱する工程と、前記混合液のpHを7〜9に調整する工程と、前記混合液を酸化する工程とを含む赤飯の素の製造方法、赤飯の素、赤飯及びその製造方法、並びに赤飯の素製造用キットである。【選択図】なし
Abstract translation: 要解决的问题：提供一种具有红米所需颜色，优良风味，抑制金属味和抑制涩味的红米添加剂及其制备方法; 使用红米添加剂的红米及其制备方法; 以及用于生产红米添加剂的红米添加剂的生产试剂盒。解决方案：红米添加剂的制备方法包括以下步骤：加热包含含豆组合物的混合液，其中含有至少任一种 小豆（Vigna angularis）和黑眼豆（（Vigna unguiculata），含碳酸钾的组合物和含铁酵母提取物的组合物; 将混合液调节至pH7至9; 并对混合液进行氧化。 还提供了红米添加剂，红米及其制备方法，以及用于生产红米添加剂的试剂盒。
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公开(公告)号：JP2000102392A
公开(公告)日：2000-04-11
申请号：JP14517399
申请日：1999-05-25
Applicant: ORIENTAL YEAST CO LTD
Inventor： MATSUKAWA HIROKAZU , OKA OSAMU , FUJITA TAKESHI , SAKAKIBARA HITOSHI , SUGIYAMA TATSUO
Abstract: PROBLEM TO BE SOLVED: To obtain the subject liquid reagent having excellent storage stability in a solution state, capable of simply and accurately measuring calcium in a biological sample and other samples by making the reagent include a calcium- dependent glutaminate dehydrogenase. SOLUTION: A corn-derived calcium-dependent glutaminate dehydrogenase(GLDH) gene fragment is inserted into an expression vector to construct a plasmid to express GLDH. The plasmid is transferred to Escherichia coli to give a transformant. The transformant is cultured to produce a calcium- dependent GLDH. The product is extracted and purified to give a corn-derived recombinant GLDH. Then the corn-derived calcium-dependent recombinant GLDH is mixed with a chelating agent as a controlling element to obtain concentration-dependent activation response to serum calcium concentration range to prepare an aqueous solution. Consequently the objective liquid reagent for measuring calcium, having excellent storage stability in a solution state is obtained.
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公开(公告)号：JP2000041680A
公开(公告)日：2000-02-15
申请号：JP21756998
申请日：1998-07-31
Applicant: ORIENTAL YEAST CO LTD , IMANAKA TADAYUKI
Inventor： TERAJIMA CHIKAKO , UCHIDA KOJI , MATSUKAWA HIROKAZU , OKA OSAMU , FUJITA TAKESHI , IMANAKA TADAYUKI
Abstract: PROBLEM TO BE SOLVED: To obtain, in a large amount, heat-resistant glutamate dehydrogenase(GLDH), which is useful, for example, as an reagent for determining ammonia, stable even at an alkaline solution by culturing a transformant containing recombinant glutamate dehydrogenase gene with feeding a substrate and so on at a controlled concentration of dissolved oxygen. SOLUTION: This enzyme is obtained by (1) extracting genomic DNA from Pyrococcus sp. strain KOD1 (FERM P-15070) by the conventional method, (2) obtaining GLDH gene by carrying out PCR using primers having sequences of parts having high homology to the amino acid sequence of GLDH derived from other archaea, followed by (3) culturing a transformant, which was transformed with a recombinant GLDH gene prepared by incorporating the gene into an expression vector, with continuously feeding a substrate, vitamins, and nitrogen sources such as amino acids and, if necessary, at a continuously controlled concentration of dissolved oxygen.
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公开(公告)号：JPH04234900A
公开(公告)日：1992-08-24
申请号：JP13325991
申请日：1991-05-10
Applicant: NISSHIN FLOUR MILLING CO , ORIENTAL YEAST CO LTD
Inventor： MAEDA KOJI , OKA OSAMU
Abstract: PURPOSE:To provide the subject new substance consisting of a protein extracted from wheat seeds, etc., and having respectively specified ultraviolet absorption value, molecular weight, electrophoretic mobility and amino acid composition, showing a weak pancreatic amylase inhibition and capable of inhibiting salivary gland alpha-amylase. CONSTITUTION:Water is added to wheat flour and stirred for one hour. The resultant mixture is centrifuged and the supernatant is collected. The collected supernatant is concentrated and heat treated at 70 deg.C for 30min and ethanol is then added thereto till 60vol.% concentration. The generated precipitate is removed and ethanol is added thereto till 90vol.% concentration. The resultant mixture is allowed to stand overnight to form a precipitate, thus obtaining the objective alpha-amylase inhibitor consisting of a protein, soluble in water and a dilute salt solution, insoluble in methanol, ethanol, acetone, chloroform and hexane, having ultraviolet E (1%)=12.8(1cm in aqueous solution) and 23000-23800 molecular weight (ultracentrifugation) and showing a saturation curve between human salivary gland alpha-amylase, pancreatic amylase and this inhibitor where the ratio of the amount of this inhibitor required for 50% inhibition of the former amylase to that of the later amylase is 1:2500.
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