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    METHODS FOR DIGITAL PCR
    21.
    发明公开
    METHODS FOR DIGITAL PCR 有权
    VERFAHRENFÜRDIE DIGITALE PCR

    公开(公告)号：EP2321427A2

    公开(公告)日：2011-05-18

    申请号：EP09804312.8

    申请日：2009-08-11

    Applicant: Stokes Bio Limited

    Inventor： DAVIES, Mark ,  DALTON, Tara

    IPC: C12Q1/68 ,  G01N35/08 ,  B01L3/00

    CPC classification number: C12Q1/6851 ,  B01L3/502784 ,  B01L7/525 ,  B01L2300/0838 ,  B01L2300/0867 ,  B01L2400/0487 ,  C12Q2561/101 ,  C12Q2563/159 ,  C12Q2565/629

    Abstract: The invention provides methods of conducting a nucleic acid reaction, including methods for performing digital PCR using a “droplet-in-oil” technology. In the methods, the starting sampled is segmented at least partially into a set of sample droplets each containing on average about one or fewer copies of a target nucleic acid. The droplets are passed in a continuous flow of immiscible carrier fluid through a channel that passes through a thermal cycler, whereby the target is amplified. In one implementation, the droplets are about 350 nl each and the number of positively amplified droplets is counted at the near-saturation point.

    Abstract translation: 本发明提供进行核酸反应的方法，包括使用“油滴技术”进行数字PCR的方法。 在所述方法中，起始采样至少部分地被分段成一组样品液滴，每个样品液滴平均含有约一个或更少个靶核酸拷贝。 液滴通过穿过热循环仪的通道在不混溶的载体流体的连续流中通过，从而靶扩增。 在一个实施方式中，液滴各自为约350nl，并且在近饱和点处计数积极扩增的液滴的数量。

    A MICROFLUIDIC ANALYSIS SYSTEM
    22.
    发明公开
    A MICROFLUIDIC ANALYSIS SYSTEM 有权
    MIKROFLUIDANALYSESYSTEM

    公开(公告)号：EP1663497A1

    公开(公告)日：2006-06-07

    申请号：EP04770390.5

    申请日：2004-09-06

    Applicant: Stokes Bio Limited

    Inventor： DAVIES, Mark ,  DALTON, Tara

    IPC: B01L7/00 ,  B01L3/00 ,  C12Q1/68

    CPC classification number: C12Q1/686 ,  B01F5/0057 ,  B01F13/0059 ,  B01L3/50273 ,  B01L3/502776 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/0636 ,  B01L2200/0673 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0487 ,  G01N35/08 ,  G01N35/1095 ,  G01N2035/00514

    Abstract: A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.

    Abstract translation: 微流体分析系统（1）对生物样品进行聚合酶链反应（PCR）分析。 在离心机（6）中，将样品分离成DNA和RNA成分。 通过相对流动的硅油初级载体流体，通过粘性阻力实现循环，产生涡流。 生物样品离开包封在主载体流体中的离心机。 这通过流量控制器（7）泵送到热级（9）。 热级（9）具有多个微流体装置（70），每个微流体装置（70）各自具有热区（71,72,73），其中生物样品通过向热载体流体和主载体流体的热传导而被加热或冷却 。 因此，载体流体包围样品，控制其流速，并控制其温度，而不需要微量级的移动部件。

    DEVICES, SYSTEMS, AND METHODS FOR AMPLIFYING NUCLEIC ACIDS
    26.
    发明公开
    DEVICES, SYSTEMS, AND METHODS FOR AMPLIFYING NUCLEIC ACIDS 审中-公开
    用于放大核酸的装置，系统和方法

    公开(公告)号：EP2553085A2

    公开(公告)日：2013-02-06

    申请号：EP11760338.1

    申请日：2011-03-25

    Applicant: Stokes Bio Limited

    Inventor： DAVIES, Mark ,  DALTON, Tara

    IPC: C12M1/38 ,  C12Q1/68

    CPC classification number: B01L7/525 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2400/0457 ,  B01L2400/0487 ,  C12Q1/6851 ,  C12Q2565/629 ,  C12Q2561/113

    Abstract: The present invention generally relates to a devices, systems, and methods for amplifying nucleic acids in flowing droplets. In certain embodiments, the invention provides a device for amplifying nucleic acids including at least one channel through which sample droplets including nucleic acids flow, in which the nucleic acids in the droplets are optically detectable while the droplets are flowing through the channel, and a plurality of temperature zones in thermal contact with the channel, in which the zones are located at different locations along the channel and the zones are separated from each other.

    Abstract translation: 本发明总体上涉及用于扩增流动液滴中的核酸的装置，系统和方法。 在某些实施方案中，本发明提供了用于扩增核酸的装置，所述装置包括至少一个通道，包含核酸的样本液滴通过所述通道流动，其中当液滴流经通道时，液滴中的核酸可光学检测， 与所述通道热接触的温度区域，其中所述区域位于沿着所述通道的不同位置处，并且所述区域彼此分离。

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