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    Methods and apparatus for detecting bacterial growth by spectrophotometric sampling of a fiber-optic array
    25.
    发明授权
    Methods and apparatus for detecting bacterial growth by spectrophotometric sampling of a fiber-optic array 失效
    通过光纤阵列的分光光度取样检测细菌生长的方法和装置

    公开(公告)号：US5473437A

    公开(公告)日：1995-12-05

    申请号：US420885

    申请日：1995-04-11

    Applicant: Walter Blumenfeld ,  Klaus W. Berndt

    Inventor： Walter Blumenfeld ,  Klaus W. Berndt

    IPC: C12M1/34 ,  C12Q1/06 ,  G01N21/25 ,  G01N21/90 ,  G01N23/00 ,  G01N21/64 ,  G01N35/02

    CPC classification number: G01N21/253 ,  C12M41/36 ,  C12M41/46 ,  G01N2021/174 ,  G01N2201/0407 ,  G01N2201/08 ,  Y10S435/808 ,  Y10T436/113332

    Abstract: The detection of bacterial growth or the performance of other interrogative processes in multiple sample vials is accomplished using a moving rack that selectively couples optical locations to an optical excitation and detection system. The apparatus includes a drive mechanism combining agitation of the culture vials with a sequential scanning of an array of optical fibers, preferably by a spectrophotometric excitation and detection system. Selection of each culture vial provides, for example, optical detection of bacterial growth by fluorescence or other spectrophotometric measurements. A rack for holding vials is preferably the only moving assembly, and requires no mechanical or electrical interconnection with the excitation and detection system for its operation. In general, the present invention relates to the optically indexed presentation of the optical guides, to many remote sample sites in the form of a geometric array at the interface between the moving culture vial rack assembly and the spectrophotometric excitation and detection system fixed to the body of the instrument. Methods of transmitting and receiving electromagnetic radiation to and from sample vials held in a movable rack include the steps of coupling the sample vials to an optical fiber and terminating the optical fiber at an optical coupling location. The rack is then moved to align at least one of the coupling locations with an excitation and detection system.

    Abstract translation: 使用选择性地将光学位置耦合到光学激发和检测系统的移动架来实现多个样品瓶中细菌生长或其他询问过程的性能的检测。 该装置包括驱动机构，其结合培养小瓶的搅动和光纤阵列的顺序扫描，优选通过分光光度激发和检测系统。 每个培养瓶的选择提供例如通过荧光或其他分光光度测量法光学检测细菌生长。 用于保持小瓶的支架优选地是唯一的移动组件，并且不需要与用于其操作的激励和检测系统的机械或电气互连。 一般来说，本发明涉及光学引导件的光学索引呈现，在移动的培养瓶架组件和固定在身体上的分光光度激发和检测系统之间的界面处的几何阵列形式的许多远程采样部位 的仪器。 向携带在可移动机架中的样品瓶传送和接收电磁辐射的方法包括将样品瓶连接到光纤并在光耦合位置端接光纤的步骤。 然后移动机架以使至少一个耦合位置与激励和检测系统对准。

    Method and instrument for measuring endotoxin
    29.
    发明专利
    Method and instrument for measuring endotoxin 失效
    测量内毒素的方法和仪器

    公开(公告)号：JPS6111641A

    公开(公告)日：1986-01-20

    申请号：JP13244584

    申请日：1984-06-27

    Applicant: Wako Pure Chem Ind Ltd

    Inventor： SAKATA YOSHITSUGU ,  OOISHI HARUKI ,  HATAYAMA TAIDOU ,  SHIRAISHI HIROKI ,  YANAGISAWA KAZUYA

    IPC: G01N21/75 ,  G01N21/82 ,  G01N33/579

    CPC classification number: G01N21/82 ,  G01N2201/0407

    Abstract: PURPOSE:To determine quantitatively and exactly endotoxin in a short period from a preliminarily obtd. calibration curve by measuring the time until the ratio between the max. quantity of the transmitted light after mixing of plural samples each mixed with an endotoxin gelation reagent and the quantity of the transmitted light decreasing with time attains a specified value. CONSTITUTION:The samples prepd. by adding a limulus amebocyte lysate (abbreviated as LAL) as the endotoxin gelation reagent to >=2 liquids to be tested are put respectively into cuvettes 1 to measure the endotoxin in raw material water for injections, etc. and the washing water, etc. during the process of production. Light is irradiated from a light source 5 to the respective cuvettes 1 by using a device which has many curvette holders 7 and can hold the cuvettes at a constant temp. The time until the ratio between the max. quantity of the transmitted light after mixing and the quantity of the light decreasing with time attains the specified value is measured by a detector 6 such as a photoelectric cell. A switch 8 for instructing the start of the measurement, an LED 9 for displaying the condition, an LED 10 for discriminating and displaying the gelation, an LED 11 for displaying the gelation time, etc. are provided to the device and the result of the measurement is displayed or recorded by a printer. The exact and simultaneous measurement of a trace amt. of the endotoxin in the many samples in a short period is thus made possible.

    Abstract translation: 目的：从初步确定的短期内确定定量和准确的内毒素。 校准曲线通过测量时间直到最大值之间的比例。 与内毒素凝胶试剂混合的多个样品混合后的透射光量和随时间减少的透射光量达到规定值。 规定：样品制备 分别加入鲎血细胞溶解物（缩写为LAL）作为内毒素凝胶化试剂至> = 2待测液体，分别置入比色皿1中，测定注射用原水等洗涤水等的内毒素。 在生产过程中。 通过使用具有许多弯曲保持器7的装置将光从光源5照射到各个比色皿1，并且可以将比色皿保持在恒定温度。 时间直到最大值之间的比例 通过诸如光电池的检测器6测量混合后的透射光量和随时间的减少的光量达到规定值。 用于指示开始测量的开关8，用于显示条件的LED 9，用于鉴别和显示凝胶化的LED 10，用于显示凝胶化时间的LED 11等等，并且其结果 测量由打印机显示或记录。 准确和同时测量痕迹。 的许多样品中的内毒素在短时间内成为可能。

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