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    STRAIGHTFLOW SYSTEM
    31.
    发明申请
    STRAIGHTFLOW SYSTEM 审中-公开
    直流系统

    公开(公告)号：WO2003013703A1

    公开(公告)日：2003-02-20

    申请号：PCT/US2002/024663

    申请日：2002-08-02

    Applicant: ACLARA BIOSCIENCES, INC. ,  KAO, Hung, Pin ,  MAHER, Kevin

    Inventor： KAO, Hung, Pin ,  MAHER, Kevin

    IPC: B01D57/02

    CPC classification number: G01N27/44791 ,  B01D57/02 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/563 ,  B01L2200/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2400/0415 ,  B01L2400/084 ,  G01N27/44721 ,  G01N27/44782

    Abstract: A microfabricated device, manifold system and method for electrokinetically moving biological and chemical samples including a multiplexed detection chamber for receiving a plurality of adjacent sample streams to be detected. The device includes a plurality of adjacent input channels connected to the detection chamber via an enlarged section having an expanding taper at its widest end or other shapes so that adjacent sample streams enter the detection chamber and remain discrete for a threshold distance to the detection chamber to minimize lateral diffusion.

    Abstract translation: 用于电动移动生物和化学样品的微加装置，歧管系统和方法，包括用于接收待检测的多个相邻样品流的多路复用检测室。 该装置包括多个相邻的输入通道，该多个相邻的输入通道经由扩大部分连接到检测室，该扩大部分在其最宽端或其他形状具有扩张锥形，使得相邻的样品流进入检测室并保持离散以到达检测室的阈值距离 最小化横向扩散。

    METHODS AND COMPOSITIONS FOR ANALYZING PROTEINS
    32.
    发明申请
    METHODS AND COMPOSITIONS FOR ANALYZING PROTEINS 审中-公开
    分析蛋白质的方法和组合物

    公开(公告)号：WO2002095356A2

    公开(公告)日：2002-11-28

    申请号：PCT/US2002/016098

    申请日：2002-05-21

    Applicant: ACLARA BIOSCIENCES, INC. ,  SINGH, Sharat ,  SALIMI-MOOSAVI, Hossein ,  TAHIR, Syed, Hasan ,  WALLWEBER, Gerald, J. ,  KIRAKOSSIAN, Hrair ,  MATRAY, Tracy, J. ,  HERNANDEZ, Vincent, S.

    Inventor： SINGH, Sharat ,  SALIMI-MOOSAVI, Hossein ,  TAHIR, Syed, Hasan ,  WALLWEBER, Gerald, J. ,  KIRAKOSSIAN, Hrair ,  MATRAY, Tracy, J. ,  HERNANDEZ, Vincent, S.

    IPC: G01N

    CPC classification number: G01N33/6842 ,  C07K16/00 ,  C07K2317/40 ,  G01N33/582 ,  G01N33/6803

    Abstract: Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification. A mixture comprising the sample and a first reagent comprising a cleavage-inducing moiety and a first binding agent for a binding site on a target polypeptide is subjected to conditions under which binding of respective binding moieties occurs. The binding site is the result of post-translational modification activity involving the target polypeptide. The method may be employed to determine the target polypeptide itself. In another embodiment the presence and/or amount of the target polypeptide is related to the presence and/or amount and/or activity of an agent such as an enzyme involved in the post-translational modification of the target polypeptide. The interaction between the first binding agent and the binding site brings the cleavage-inducing moiety into close proximity to a cleavable moiety, which is associated with the polypeptide and is susceptible to cleavage only when in proximity to the cleavage-inducing moiety. In this way, an electrophoretic tag for each of the polypeptides may be released. Released electrophoretic tags are separated and the presence and/or amount of the target polypeptides are determined based on the corresponding electrophoretic tags.

    Abstract translation: 公开了用于确定样品中一种或多种靶多肽已经经历了翻译后修饰的方法，组合物和试剂盒。 包含样品和包含切割诱导部分的第一试剂和用于靶多肽上的结合位点的第一结合剂的混合物经受各自结合部分的结合发生的条件。 结合位点是涉及靶多肽的翻译后修饰活性的结果。 该方法可用于确定靶多肽本身。 在另一个实施方案中，靶多肽的存在和/或量与试剂例如涉及靶多肽的翻译后修饰的酶的存在和/或量和/或活性有关。 第一结合剂和结合位点之间的相互作用导致切割诱导部分紧密接近可切割部分，其可与多肽相关，并且仅在接近切割诱导部分时易于切割。 以这种方式，可以释放每个多肽的电泳标签。 分离出释放的电泳标签，并且基于相应的电泳标签确定靶多肽的存在和/或量。

    ANALYZING PHOSPHORYLATED PROTEINS
    33.
    发明申请
    ANALYZING PHOSPHORYLATED PROTEINS 审中-公开

    公开(公告)号：WO2002094998A3

    公开(公告)日：2002-11-28

    申请号：PCT/US2002/016100

    申请日：2002-05-21

    Applicant: ACLARA BIOSCIENCES, INC. ,  JOHNSON AND JOHNSON PHARMACEUTICAL RESEARCH AND DEVELOPMENT, LLC ,  SINGH, Sharat ,  ZIVIN, Robert, A.

    Inventor： SINGH, Sharat ,  ZIVIN, Robert, A.

    IPC: G01N33/543

    Abstract: Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone phosphorylation. A mixture comprising the sample and a first reagent comprising a cleavage-inducing moiety and an IMAC resin for a binding site on a target polypeptide is subjected to conditions under which binding of respective binding moieties occurs. The binding site is the result of phosphorylation activity involving the target polypeptide. The method may be employed to determine the target polypeptide itself. In another embodiment the presence and/or amount of the target polypeptide is related to the presence and/or amount and/or activity of an agent such as an enzyme involved in phosphorylation of the target polypeptide. The interaction between the IMAC resin and the binding site brings the cleavage-inducing moiety into close proximity to a cleavable moiety, which is associated with the polypeptide and is susceptible to cleavage only when in proximity to the cleavage-inducing moiety. In this way, an electrophoretic tag for each of the polypeptides may be released. Released electrophoretic tags are separated and the presence and/or amount of the target polypeptides are determined based on the corresponding electrophoretic tags.

    MONOOXYGENASE ASSAYS
    34.
    发明申请
    MONOOXYGENASE ASSAYS 审中-公开
    MONOOXYGENASE测试

    公开(公告)号：WO0173113A2

    公开(公告)日：2001-10-04

    申请号：PCT/US0110155

    申请日：2001-03-29

    Applicant: ACLARA BIOSCIENCES INC

    Inventor： SINGH SHARAT ,  CHENNA AHMED ,  SALIMI-MOOSARI HOSSEIN ,  GIBBONS IAN

    IPC: C12Q1/26

    CPC classification number: C12Q1/26 ,  G01N2333/795 ,  G01N2333/80 ,  G01N2333/90245 ,  Y10S435/968

    Abstract: Cytochrome P-450 assay methods and kits for the methods are provided employing a cytochrome P-450 enzyme, substrates characterized by having an oxidizable methylene group oxidized to an aldehyde and a fluorescent hydrazine. A fluorescent hydrazine is added to the reaction mixture and the resulting hydrazone analyzed by capillary electrophoresis. The method finds use in evaluating compounds for enzyme modulating activity.

    Abstract translation: 使用细胞色素P-450酶提供细胞色素P-450测定方法和用于该方法的试剂盒，其特征在于具有氧化成醛的可氧化亚甲基基团和荧光肼。 向反应混合物中加入荧光肼，通过毛细管电泳分析得到的腙。 该方法用于评价化合物的酶调节活性。

    MULTIPLEXED STRAND DISPLACEMENT FOR NUCLEIC ACID DETERMINATIONS
    36.
    发明申请
    MULTIPLEXED STRAND DISPLACEMENT FOR NUCLEIC ACID DETERMINATIONS 审中-公开
    用于核酸测定的多重条带位移

    公开(公告)号：WO0106008A2

    公开(公告)日：2001-01-25

    申请号：PCT/US0018301

    申请日：2000-06-30

    Applicant: ACLARA BIOSCIENCES INC

    Inventor： SINGH SHARAT ,  INAMDAR ANITA ,  ULLMAN EDWIN F ,  CAO LICHING ,  ALBAGLI DAVID

    IPC: C12N15/09 ,  C12Q1/68

    CPC classification number: C12Q1/6844

    Abstract: Multiplexed determinations of large numbers of events are achieved in an accurate and simple manner by using a combination of different oligonucleotides, which serve for both capture and release from a support and strand displacement oligonucleotides for sequential release of groups of oligonucleotides. Also included, as part of the oligonucleotide reagents may be identifiers, which serve to identify a particular characteristic. The method is illustrated using primers for initiation of chain extension joined to or serving as the capture/release sequence, where the extended primer has an identifier. After extending the primer, the extended primers are captured and independently released and the released extended primers assayed. The subject method finds application for nucleic acid sequence determinations, single nucleotide polymorphism determinations, identification of nucleic acid fragments, and the like.

    Abstract translation: 通过使用不同寡核苷酸的组合，以准确和简单的方式实现大量事件的多重测定，其用于捕获和释放支持物和链置换寡核苷酸以顺序释放寡核苷酸组。 还包括，作为寡核苷酸试剂的一部分，可以是用于鉴定特定特征的标识符。 使用引物连接或用作捕获/释放序列的引物引物的方法，其中扩增引物具有标识符。 扩增引物后，扩增引物被捕获并独立释放，并测定释放的扩增引物。 本发明方法用于核酸序列测定，单核苷酸多态性测定，核酸片段鉴定等。

    37.
    发明专利
    未知

    公开(公告)号：AT347692T

    公开(公告)日：2006-12-15

    申请号：AT97937078

    申请日：1997-07-30

    Applicant: ACLARA BIOSCIENCES INC

    Inventor： NELSON ROBERT J ,  HOOPER HERBERT H ,  HAUSER ALAN K ,  SASSI ALEXANDER P

    IPC: G01N27/447

    Abstract: Integrated electrophoretic microdevices comprising at least an enrichment channel and a main electrophoretic flowpath are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said main electrophoretic flowpath through a discharge outlet. The subject devices find use in a variety of electrophoretic applications, including clinical assays.

    PROCEDIMIENTO PARA LA UNION LIBRE DE SUSTANCIAS AJENAS DE DOS PIEZAS DE MATERIAL PLASTICO.
    38.
    发明专利
    PROCEDIMIENTO PARA LA UNION LIBRE DE SUSTANCIAS AJENAS DE DOS PIEZAS DE MATERIAL PLASTICO. 未知

    公开(公告)号：ES2257325T3

    公开(公告)日：2006-08-01

    申请号：ES00967644

    申请日：2000-09-02

    Applicant: ACLARA BIOSCIENCES INC

    Inventor： NEYER ANDREAS ,  JOHNCK MATTHIAS

    IPC: B29C65/00 ,  B29C65/02 ,  B29C71/04 ,  C08J5/12

    Abstract: Procedimiento para la unión libre de sustancias ajenas de dos piezas (2, 3; 5, 6) mutuamente adyacentes de material plástico, al mismo tiempo, que cada pieza posee una superficie de contacto prevista a través de la que se unen las piezas y que al menos una pieza posee en la superficie de contacto cavidades con microestructura y/o nanoestructura, caracterizado porque - la superficie (K) de contacto prevista de al menos una de las dos piezas (2, 3, respectivamente 5, 6), con la que esta limita con la otra pieza (3, 6, respectivamente 2, 5) en la unión se expone, al menos por zonas, a una radiación rica en energía, de manera, que se reduzca la temperatura de transición vítrea en una capa (R, R'') del borde, - las dos piezas (2, 3; 5, 6)son llevadas a su posición funcional mutua y - a continuación se calienta, para el establecimiento de la unión de las piezas (2, 3; 5, 6) al menos la capa (R, R'') del borde modificada con el proceso de irradiación al menos en la zona de su superficie hasta una temperatura, que se halle por encima de la temperatura de transición vítrea de la capa (R, R'') del borde modificada con la irradiación, pero por debajo de la temperatura de transición vítrea de las zonas no modificadas de la pieza (2, 3; 5, 6) correspondiente, de manera, que las cavidades con microestructuras y/o nanoestructuras conserven su estabilidad de formas.

    40.
    发明专利
    未知

    公开(公告)号：DE60106053T2

    公开(公告)日：2005-11-17

    申请号：DE60106053

    申请日：2001-07-20

    Applicant: ACLARA BIOSCIENCES INC

    Inventor： LACKRITZ S ,  CRUZADO INGRID ,  TAN R

    IPC: G01N33/50 ,  B81B1/00 ,  G01N27/447 ,  G01N37/00

    Abstract: Capillary electrophoresis is performed under conventional conditions in microchannels having a norbornene based polymer surface. The norbornene based polymers can be used as a solid substrate for forming the necessary features for a microfluidic device, where the entire device may be made of norbornene based polymer. Conveniently, a norbornene based polymer layer having a lower glass transition temperature may be used to adhere a cover or enclosing layer to the substrate to enclose the microchannels and provide a bottom for the reservoirs.

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