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    PROTEIN SEPARATIONS USING AN ACRYLAMIDE CONTAINING FILTER
    31.
    发明申请
    PROTEIN SEPARATIONS USING AN ACRYLAMIDE CONTAINING FILTER 审中-公开
    蛋白质分离使用含有丙烯酰胺的过滤器

    公开(公告)号：WO2015088677A9

    公开(公告)日：2016-04-28

    申请号：PCT/US2014064230

    申请日：2014-11-06

    Applicant: EMD MILLIPORE CORP

    Inventor： KOZLOV MIKHAIL ,  CATALDO WILLIAM ,  CARON JEFFREY

    IPC: B01J20/285 ,  C07K1/14 ,  C08F20/56

    CPC classification number: C07K1/34 ,  B01D15/10 ,  B01D37/00 ,  B01J20/267 ,  B01J20/288 ,  B01J20/289 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/327 ,  B01J20/3276 ,  B01J20/3291 ,  B01J39/26 ,  B01J2220/80 ,  C07K1/18 ,  C07K16/00 ,  C08F220/18 ,  C08F220/54 ,  C08F220/56 ,  C08F220/58 ,  C08F259/08 ,  C08F2220/1825 ,  C08F2220/1858 ,  C08J5/2243 ,  C08J2351/00

    Abstract: Novel compositions for removing impurities such as, protein aggregates, from a sample containing a protein of interest, e.g., an antibody. Such compositions can be used prior to the virus filtration step during protein purification, to remove aggregates and protect the virus filter from fouling, therefore improving virus filter capacity. A porous solid support including a co-polymer having at least two monomers, wherein at least one of the monomers comprises acrylamide and at least a second monomer comprises a hydrophobic binding group, where the solid support selectively binds protein aggregates, thereby to separate the monomeric protein of interest from the protein aggregates. The method can be performed under neutral to high pH and high conductivity conditions.

    Abstract translation: 用于从含有目的蛋白质的样品例如抗体中除去杂质如蛋白质聚集体的新型组合物。 这样的组合物可以在蛋白质纯化期间的病毒过滤步骤之前使用，以除去聚集体并保护病毒过滤器免于结垢，从而提高病毒过滤能力。 一种多孔固体支持物，包括具有至少两种单体的共聚物，其中所述单体中的至少一种包含丙烯酰胺，并且至少第二单体包含疏水性结合基团，其中所述固体支持物选择性地结合蛋白质聚集体，由此分离单体 来自蛋白质聚集体的目标蛋白质。 该方法可在中性至高pH和高电导条件下进行。

    2D LOW LEVEL MIXING BAG FOR STORAGE AND SHIPPING
    33.
    发明申请
    2D LOW LEVEL MIXING BAG FOR STORAGE AND SHIPPING 审中-公开
    2D低水平混合袋用于存储和运输

    公开(公告)号：WO2014085035A3

    公开(公告)日：2015-07-16

    申请号：PCT/US2013068375

    申请日：2013-11-05

    Applicant: EMD MILLIPORE CORP

    Inventor： MORRISSEY MARTIN ,  PEREIRA BRIAN

    IPC: B01F13/08

    CPC classification number: B01F15/0085 ,  B01F7/00241 ,  B01F13/0827 ,  B01F15/00487 ,  B01F2215/0034 ,  B01F2215/0073 ,  C12M23/14 ,  C12M27/02

    Abstract: A disposable container, such as a deformable bag, for a fluid, having one or more inlets and one or more outlets and an impeller assembly within the container to cause mixing, dispersing, homogenizing and/or circulation of one or more ingredients contained or added to the container. The region within the container that can contain liquid is funnel shaped, which allows very low fluid level mixing, dispensing while mixing, and a reduction or elimination of vortex formation.

    Abstract translation: 用于流体的一次性容器，例如可变形袋，具有一个或多个入口和一个或多个出口以及容器内的叶轮组件，用于引起包含或添加的一种或多种成分的混合，分散，均化和/或循环 到容器 容器内可容纳液体的区域是漏斗形的，其允许非常低的液面混合，分配同时混合，以及减少或消除涡流形成。

    DISPOSABLE DIRECT CAPTURE DEVICE
    34.
    发明申请
    DISPOSABLE DIRECT CAPTURE DEVICE 审中-公开
    可替代的直接捕获设备

    公开(公告)号：WO2014120344A3

    公开(公告)日：2015-01-22

    申请号：PCT/US2013074857

    申请日：2013-12-13

    Applicant: EMD MILLIPORE CORP

    Inventor： CACACE BENJAMIN

    IPC: G01N1/10 ,  C12N15/10 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/68

    CPC classification number: G01N1/34 ,  B01J39/26 ,  B01J41/20 ,  B01J47/014 ,  B01J47/022 ,  B01J47/127 ,  B01L3/505 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/123 ,  C07K1/14 ,  C07K1/36 ,  G01N1/405

    Abstract: The invention relates to a liquid sample preparation device such as a disposable, collapsible, flexible polymeric bag containing an adsorptive curtain of a functionalized shaped polymeric fiber bed for the direct capture of biomolecules from liquid samples. The functionalized shaped polymeric fiber bed includes fibrillated, ridged or winged-shaped fiber structures that significantly increases the surface area of the fiber resulting in enhanced separation, retention and/or purification of liquid samples containing biomolecules of interest as the liquid samples contact the adsorptive curtain of functionalized shaped fibers. Liquid samples include unclarified liquid feeds or other liquids containing one or more biomolecules of interest, including, but not limited to vaccines, recombinant proteins, cells, stem cells, monoclonal antibodies (mAbs), proteins, antibody, peptides, oligopeptides, nucleic acids, oligonucleotides, RNA, DNA, oligosaccharides and polysaccharides.

    Abstract translation: 本发明涉及一种液体样品制备装置，例如一次性可折叠的柔性聚合物袋，其包含用于从液体样品直接捕获生物分子的官能化成形聚合物纤维床的吸附帘。 功能化的聚合物纤维床包括原纤化的，脊状的或翼状的纤维结构，其显着增加纤维的表面积，导致当液体样品接触吸附窗帘时，含有生物分子的液体样品的增强的分离，保留和/或纯化 的功能化成形纤维。 液体样品包括未澄清的液体进料或含有一种或多种感兴趣的生物分子的其它液体，包括但不限于疫苗，重组蛋白，细胞，干细胞，单克隆抗体（mAb），蛋白质，抗体，肽，寡肽，核酸， 寡核苷酸，RNA，DNA，寡糖和多糖。

    Method for purifying biomolecule from mixture containing impurity
    35.
    发明专利
    Method for purifying biomolecule from mixture containing impurity 有权
    用于从含有混合物的混合物中净化生物分子的方法

    公开(公告)号：JP2013155187A

    公开(公告)日：2013-08-15

    申请号：JP2013083213

    申请日：2013-04-11

    Applicant: Emd Millipore Corp ,  イー・エム・デイー・ミリポア・コーポレイシヨン

    Inventor： WILSON MOYA ,  DUPONT ALISON

    IPC: C07K1/14 ,  C07K16/00

    CPC classification number: C12M27/00 ,  C12M23/20 ,  C12M23/28 ,  C12M47/12

    Abstract: PROBLEM TO BE SOLVED: To provide a method for the efficient purification of samples, particularly those containing biomolecules, wherein one or more process steps that can result in contamination or material loss is reduced or eliminated.SOLUTION: A method for purifying a biomolecule from a mixture containing impurities includes: providing the mixture at a set of conditions; adding one or more polymers, soluble in the mixture under the set of conditions and capable of reversibly and selectively binding to the biomolecule; mixing the one or more solubilized polymers throughout the mixture; precipitating the one or more polymers and bound biomolecule out of solution as precipitates by changing the set of conditions in the mixture; washing the precipitate by contacting the precipitate with a wash solution and filtering the supernatant through a first membrane; and recovering the bound biomolecule from the polymer and filtering the biomolecule through a second membrane.

    Abstract translation: 要解决的问题：提供用于有效净化样品的方法，特别是含有生物分子的方法，其中可以减少或消除可能导致污染或材料损失的一个或多个工艺步骤。解决方案：一种用于从生物分子中纯化生物分子的方法 含有杂质的混合物包括：在一组条件下提供混合物; 在一组条件下加入可溶于混合物并能够可逆地和选择性地结合生物分子的一种或多种聚合物; 将一种或多种溶解的聚合物混合在整个混合物中; 通过改变混合物中的条件来沉淀一种或多种聚合物并将生物分子从溶液中结合为沉淀物; 通过将沉淀物与洗涤溶液接触并通过第一膜过滤上清液来洗涤沉淀物; 并从聚合物中回收结合的生物分子，并通过第二个膜过滤生物分子。

    Centrifugal filter
    36.
    发明专利
    Centrifugal filter 有权
    离心过滤器

    公开(公告)号：JP2013128924A

    公开(公告)日：2013-07-04

    申请号：JP2013019834

    申请日：2013-02-04

    Applicant: Emd Millipore Corp ,  イー・エム・デイー・ミリポア・コーポレイシヨン

    Inventor： BONHOMME LOUIS ,  DOYLE JOHN ,  GREENIZEN KURT ,  GAGNE GEORGE ,  DEVIN SEAN ,  LOMBARD GERARD ,  SMITH VINCENT

    IPC: B04B5/02 ,  B01D29/01 ,  B01D61/18 ,  B04B11/00

    CPC classification number: B01D63/16 ,  B01D61/145 ,  B01D61/18 ,  B01D63/08 ,  B01D2313/08 ,  B01L3/502 ,  B01L3/5021 ,  B01L3/5635 ,  B01L2300/0681

    Abstract: PROBLEM TO BE SOLVED: To provide a filtration device suited for concentration of liquid samples, particularly biomolecules, and a method of concentrating, desalting, purifying and/or fractionating liquid samples.SOLUTION: The device includes a housing having a sample reservoir, and two substantially vertically oriented and spaced apart membranes disposed in the housing. An underdrain is associated with each membrane such that fluid passing through each membrane flows through a respective underdrain into a filtrate collection chamber. The fluid that does not pass through the membrane is collected in the retentate collection chamber, and can be recovered such as by a reverse spinning step, achieving recoveries greater than about 90%. The substantially vertical orientation of the membranes increases the available membrane area by at least 2.7 times the area available in a conventional filter device. The two-panel configuration also maintains more available membrane area in use during the last stages of filtration than a one-panel configuration.

    Abstract translation: 要解决的问题：提供适合于浓缩液体样品，特别是生物分子的过滤装置，以及浓缩，脱盐，净化和/或分馏液体样品的方法。解决方案：该装置包括具有样品储存器的壳体和两个 设置在壳体中的基本垂直取向和间隔开的膜。 与每个膜相关联的下划线，使得通过每个膜的流体通过相应的下划线流入滤液收集室。 不通过膜的流体被收集在滞留物收集室中，并且可以通过反转纺步骤回收，实现大于约90％的回收率。 膜的基本垂直取向将可用的膜面积增加了传统过滤装置中可用面积的至少2.7倍。 两面板配置在过滤的最后阶段比在单面板配置中还维持使用更多的可用膜面积。

    Electrodeionization method with hydrodynamic flow splitting and device therefor
    40.
    发明专利
    Electrodeionization method with hydrodynamic flow splitting and device therefor 有权
    具有水动力分流的电极化方法及其装置

    公开(公告)号：JP2012179605A

    公开(公告)日：2012-09-20

    申请号：JP2012134014

    申请日：2012-06-13

    Applicant: Emd Millipore Corp ,  イー・エム・デイー・ミリポア・コーポレイシヨン

    Inventor： GRABOWSKI ANDREJ

    IPC: C02F1/469 ,  B01D61/48

    CPC classification number: B01D57/02 ,  B01D61/48 ,  C02F1/44 ,  C02F1/4695 ,  C02F2001/46119 ,  C02F2201/4611 ,  C02F2201/46115 ,  C02F2301/043

    Abstract: PROBLEM TO BE SOLVED: To provide a method of removing ions and ionizable substances from a polar liquid (10) including at least one process, and to provide a device therefor.SOLUTION: In the method, the polar liquid (10) is split into a first stream (F1) and a second stream (F2), the first stream (F1) passes through an ion exchange material (2) positioned between two electrodes (4, 5) to which an electric field is applied that can be electrochemically regenerated, and the first stream (F1) flows from one electrode (4) to the other electrode (5) to move ions that are removed in a reverse direction to the first stream penetrating the ion exchange material (2), and the second stream (F2) washes the one electrode (4), and the material is regenerated with the ions that are formed on the other electrode (5). There is also provided the device for executing the method.

    Abstract translation: 要解决的问题：提供从包括至少一种方法的极性液体（10）中除去离子和可离子化物质的方法，并提供其装置。 解决方案：在该方法中，极性液体（10）被分成第一流（F1）和第二流（F2），第一流（F1）通过位于两个之间的离子交换材料（2） 施加电场的电极（4,5）可以进行电化学再生，第一流（F1）从一个电极（4）流到另一个电极（5），以移动沿相反方向去除的离子 到第一流穿透离子交换材料（2），第二流（F2）洗涤一个电极（4），并且用形成在另一个电极（5）上的离子再生材料。 还提供了用于执行该方法的装置。 版权所有（C）2012，JPO＆INPIT

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