公开(公告)号：WO1997012895A1

公开(公告)日：1997-04-10

申请号：PCT/JP1996002789

申请日：1996-09-26

Applicant: JAPAN SCIENCE AND TECHNOLOGY CORPORATION ,  NIPPON KAYAKU KABUSHIKI KAISYA ,  SAKURAI, Yasuhisa ,  YOKOYAMA, Masayuki ,  KATAOKA, Kazunori ,  OKANO, Teruo ,  FUKUSHIMA, Shigeto ,  UEHARA, Ryuji ,  AKUTSU, Tomoko ,  OKAMOTO, Kazuya ,  MASHIBA, Hiroko ,  MACHIDA, Megumi ,  SHIMIZU, Kazuhisa

Inventor： JAPAN SCIENCE AND TECHNOLOGY CORPORATION ,  NIPPON KAYAKU KABUSHIKI KAISYA

IPC: C07H15/252

CPC classification number: A61K9/1075

Abstract: Di-, tri- or tetramers of anthracycline compounds which can be obtained by treating anthracycline compounds having an anticancer activity with an alkali and thus chemically binding these compounds directly to each other. Medicinal preparations comprising a high-molecular block copolymer/drug complex wherein the high-molecular block copolymer composed of a structural part of a hydrophilic polymer and another structural part of a hydrophobic polymer forms a micelle having the shell made of the hydrophilic part and a di-, tri- or tetramer of an anthracycline compound is contained optionally together with other drugs in the hydrophobic core. Medicinal preparations comprising a high-molecular block copolymer/drug complex wherein a high-molecular block copolymer composed of a structural part of a hydrophilic polymer and another structural part of a hydrophobic polymer forms a micelle having the shell made of the hydrophilic part and an anthracycline anticancer agent is contained in the hydrophobic core and capable of giving one hour after the intravenous administration to CDF1 mice of plasma anthracycline anticancer agent level (% of dose/ml of mouse plasma) of at least 10, when the anthracycline anticancer agent level in the preparation administered is taken as 100. These medicinal preparations have a potent drug effect but a low toxicity.

Abstract translation: 蒽环类化合物的二，三或四聚体可以通过用碱处理具有抗癌活性的蒽环类化合物，从而将这些化合物彼此直接化学结合而获得。 包含高分子嵌段共聚物/药物复合物的药物制剂，其中由亲水性聚合物的结构部分和疏水性聚合物的另一结构部分构成的高分子嵌段共聚物形成具有由亲水部分制成的壳的胶束和二 - 蒽环类化合物的三或四聚物任选与其它药物一起含在疏水核心中。 包含高分子嵌段共聚物/药物复合物的药物制剂，其中由亲水性聚合物的结构部分和疏水性聚合物的另一结构部分构成的高分子嵌段共聚物形成具有由亲水部分制成的壳的胶束和蒽环类 抗癌剂包含在疏水性核心中，并且能够在静脉内给药1小时后给予血浆蒽环类抗癌药水平（小鼠血浆的剂量/ ml）至少10的CDF1小鼠，当蒽环霉素抗癌剂水平在 给药的制剂为100.这些药物制剂具有有效的药物作用，但毒性低。

公开(公告)号：WO2001066556A1

公开(公告)日：2001-09-13

申请号：PCT/JP2001001670

申请日：2001-03-05

Applicant: JAPAN SCIENCE AND TECHNOLOGY CORPORATION ,  SAITO, Isao ,  FUJIMOTO, Kenzo ,  MATSUDA, Shigeo ,  YOSHINO, Hideaki

Inventor： JAPAN SCIENCE AND TECHNOLOGY CORPORATION

IPC: C07H21/02

CPC classification number: C07H21/00 ,  C07H19/10 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q2525/117 ,  C12Q2523/313

Abstract: Nucleic acids having a novel base whereby nucleic acids can be reversibly ligated to each other by light-irradiation; nucleic acids wherein these nucleic acids have been immobilized; a method of conveniently producing branched nucleic acids and capped nucleic acids by using the above nucleic acids; a method of immobilizing nucleic acids on a solid support by using light without resort to any enzymes or chemical reagents; and a method of purifying and recovering nucleic acids and a method of identifying, detecting and quantifying nucleic acids with the use of the above method. Namely, a method of reversibly ligating nucleic acids conveniently and efficiently by using the light-reactivity of a pyrimidine base having a substituted vinyl group at the 5-position of pyrimidine; nucleic acids therefor; a method of inactivating DNA by using the same; and the like. Nucleic acids having a pyrimidine base having a substituted vinyl group at the 5-position of pyrimidine immobilized on a solid support; methods of immobilizing, purifying, recovering, identifying, detecting or quantifying nucleic acids having specific base sequences by using the above nucleic acids.

Abstract translation: 核酸具有新的碱基，其中核酸可以通过光照射彼此可逆地连接; 其中这些核酸已被固定的核酸; 通过使用上述核酸方便地产生支链核酸和封端核酸的方法; 通过使用光而不使用任何酶或化学试剂将核酸固定在固体支持物上的方法; 和核酸的纯化和回收方法以及使用上述方法鉴定，检测和定量核酸的方法。 即，通过使用嘧啶5-位上具有取代乙烯基的嘧啶碱的光反应性，方便有效地可逆地连接核酸的方法; 其核酸; 通过使用其灭活DNA的方法; 等等。 具有在固定支持物上固定有嘧啶5-位的嘧啶碱基具有取代的乙烯基的核酸; 通过使用上述核酸固定，纯化，回收，鉴定，检测或定量具有特定碱基序列的核酸的方法。

公开(公告)号：WO2001057853A1

公开(公告)日：2001-08-09

申请号：PCT/JP2001000648

申请日：2001-01-31

Applicant: JAPAN SCIENCE AND TECHNOLOGY CORPORATION ,  NAKADAI, Kazuhiro ,  OKUNO, Hiroshi ,  KITANO, Hiroaki ,  MATSUI, Tatsuya

Inventor： JAPAN SCIENCE AND TECHNOLOGY CORPORATION

IPC: G10L21/02

CPC classification number: G10L21/0208 ,  B25J13/003 ,  G10L2021/02163 ,  G10L2021/02165

Abstract: The invention provides an auditory device (10) of a robot, particularly of a human-shaped or animal-shaped robot capable actively of collecting sounds from an external target without being influenced by the internal noises, for example, generated by a drive mechanism. The device comprises a soundproof case (14) covering at least the robot head (13), a pair of external microphones (16) provided in the positions of ears outside the case to mainly collect external sounds, a pair of internal microphones (17) provided inside the case to mainly collect noises from internal noise sources, and a processing circuit (18, 19) that cancels the noises from the internal noise sources in the sound signals from the external microphones based on the signals from the external and internal microphones. The processing circuit cancels the noises in the sound signals from the external microphones by operating upon the sound signals from the external microphones and the sound signals from the internal microphones.

Abstract translation: 本发明提供了一种机器人的听觉装置（10），特别是人体形状或动物形机器人，其能够主动地收集来自外部目标的声音，而不受内部噪声的影响，例如由驱动机构产生的。 所述装置包括至少覆盖所述机器人头部的隔音箱（14），设置在所述壳体外部的耳朵位置以主要收集外部声音的一对外部麦克风（16），一对内部麦克风（17） 设置在壳体内部以主要从内部噪声源收集噪声;以及处理电路（18,19），其基于来自外部和内部麦克风的信号，从外部麦克风的声音信号中消除来自内部噪声源的噪声。 处理电路通过操作来自外部麦克风的声音信号和来自内部麦克风的声音信号来消除来自外部麦克风的声音信号中的噪声。

公开(公告)号：WO2000023586A1

公开(公告)日：2000-04-27

申请号：PCT/JP1999005757

申请日：1999-10-19

Applicant: JAPAN SCIENCE AND TECHNOLOGY CORPORATION ,  SUGAMURA, Kazuo ,  ASADA, Hiroshi

Inventor： JAPAN SCIENCE AND TECHNOLOGY CORPORATION

IPC: C12N15/12

CPC classification number: C07K14/4702

Abstract: A human protein Gfr40 containing the amino acid sequence represented by SEQ ID NO:1 which is a novel signal transfer molecule interacting with a cytokine-type signal transfer molecule AMSH; a human gene Grf40 cDNA encoding this protein which contains the base sequence represented by SEQ ID NO:2; and an antibody against the protein Grf40.

Abstract translation: 含有由SEQ ID NO：1表示的氨基酸序列的人类蛋白Gfr40，其是与细胞因子型信号转移分子AMSH相互作用的新型信号转导分子; 编码该蛋白质的人基因Grf40 cDNA，其含有SEQ ID NO：2所示的碱基序列; 和针对蛋白质Grf40的抗体。

公开(公告)号：WO1998025308A1

公开(公告)日：1998-06-11

申请号：PCT/JP1997004417

申请日：1997-12-03

Applicant: JAPAN SCIENCE AND TECHNOLOGY CORPORATION ,  MIURA, Tadao ,  TANAKA, Shun-ichiro ,  SUMIYA, Touru

Inventor： JAPAN SCIENCE AND TECHNOLOGY CORPORATION

IPC: H01L29/06

CPC classification number: H01L29/872 ,  H01L29/45 ,  H01L29/452 ,  H01L29/456 ,  H01L29/47 ,  H01L29/475

Abstract: A metal layer (2) of a thickness of 20nm or below is formed on a semiconductor layer (1) which is constituted of a semiconductor single crystal substrate, etc. The metal layer (2) is constituted of first regions (A) wherein the metal layer (2) is brought into direct contact with the semiconductor layer (1), and second regions (B) wherein intermediate layers (3) which are made of insulators, other metal than the metal layer (2), or other semiconductor than the semiconductor layer (1) and which is 10nm or below in thickness exist between the semiconductor layer (1) and the metal layer (2). The first regions (A) and the second regions (B) are different is Schottky current and the height of a Schottky barrier. Each of the regions (A, B) has a size of nano level and each interfaces in each of the regions (A, B) has a substantially uniform potential barrier. A film-like composite structure of this structure contributes much to the reduction in size of a semiconductor device to a nano level and the realization of a new functional device.

Abstract translation: 在由半导体单晶衬底等构成的半导体层（1）上形成厚度为20nm以下的金属层（2）。金属层（2）由第一区域（A） 金属层（2）与半导体层（1）直接接触，而第二区域（B），其中由绝缘体制成的中间层（3），比金属层（2）的金属或其他半导体比 在半导体层（1）和金属层（2）之间存在厚度为10nm以下的半导体层（1）。 第一区域（A）和第二区域（B）不同是肖特基电流和肖特基势垒的高度。 每个区域（A，B）具有纳米级的尺寸，并且每个区域（A，B）中的每个界面具有基本均匀的势垒。 这种结构的膜状复合结构有助于将半导体器件的尺寸减小到纳米级并实现新的功能器件。

公开(公告)号：US20140147831A1

公开(公告)日：2014-05-29

申请号：US14058885

申请日：2013-10-21

Applicant: JAPAN SCIENCE AND TECHNOLOGY CORPORATION

Inventor： NAKATSUGAWA Shigekazu

IPC: C12N5/071

CPC classification number: C12N5/0625 ,  A61K35/12 ,  A61L27/3604 ,  A61L27/3813 ,  C12N5/0697 ,  C12N2502/088 ,  C12N2502/095 ,  C12N2502/246 ,  C12N2502/256 ,  C12N2502/28 ,  C12N2502/30 ,  C12N2503/00 ,  C12N2503/04

Abstract: A normal regenerated tissue is formed by exposing to radiation a connective tissue or a supporting tissue originating in an organ to thereby form a feeder layer and then transplanting epithelial cells thereon to form a stratified structure. By conveniently and surely providing regenerated tissue by the 3-dimensional culture with the use of a human-origin normal tissue as a base, it is possible to construct systems for assessing effects and side effects of chemicals such as drugs or assessing sensitivities thereof with the use of regenerated tissues as models of corresponding tissues respectively.

Abstract translation: 通过暴露于来自器官的结缔组织或支持组织从而形成饲养层，然后在其上移植上皮细胞以形成分层结构，形成正常的再生组织。 通过使用人源性正常组织作为基础，通过三维培养方便且可靠地提供再生组织，可以构建用于评估诸如药物的化学物质的效果和副作用的系统，或者评估其敏感性 分别使用再生组织作为相应组织的模型。

公开(公告)号：US20030059419A1

公开(公告)日：2003-03-27

申请号：US10298402

申请日：2002-11-18

Applicant: Japan Science and Technology Corporation

Inventor： Shizuo Akira ,  Takahiro Shimada

IPC: A61K038/45 ,  C07H021/04 ,  C12N009/12 ,  C12P021/02 ,  C12N005/06

CPC classification number: C12N9/1205 ,  A61K38/00

Abstract: Novel IkB kinase IKK-i which is a novel serine/threonine kinase capable of activating a transcription factor NF-kB which inhibits the expression of various genes relating to immune response; a gene encoding the same; and medicinal compositions containg the same.

Abstract translation: 新型IkB激酶IKK-i，其是能够激活转录因子NF-kB的新型丝氨酸/苏氨酸激酶，其抑制与免疫应答相关的各种基因的表达; 编码该基因的基因; 和含有其的药物组合物。

公开(公告)号：US20030200567A1

公开(公告)日：2003-10-23

申请号：US10304454

申请日：2002-11-25

Applicant: National Institute of Agrobiological Sciences ,  Japan Science and Technology Corporation

Inventor： Setsuko Komatsu ,  Arun Sharma ,  Junji Hashimoto ,  Kengo Sakaguchi

IPC: A01H001/00 ,  C07H021/04 ,  C12N015/82 ,  C12N005/04

CPC classification number: C12N15/8273 ,  C07K14/415

Abstract: An objective of the present invention is to isolate and identify a gene encoding the polypeptide that interacts with calreticulin (CRT), and to provide the gene and the use thereof. Specifically, an objective of the present invention is to provide the CRTintP gene, the transformed plants comprising the CRTintP gene, and a method of producing these transformed plants. The present inventors successfully detected a group of CRT-interacting genes using the yeast two-hybrid method. As a result, the present inventors detected the polynucleotide encoding the CRT-interacting polypeptide (named as CRTintP). Furthermore, the present inventors isolated the full-length cDNA encoding the CRTintP and revealed that the gene encoding the cDNA was novel. It was found that the expression of the CRT and CRTintP genes was significantly increased following application of cold stress in rice leaves.

Abstract translation: 本发明的目的是分离和鉴定编码与钙网蛋白（CRT）相互作用的多肽的基因，并提供该基因及其用途。 具体地，本发明的目的是提供CRTintP基因，包含CRTintP基因的转化植物以及这些转化植物的生产方法。 本发明人使用酵母双杂交方法成功地检测了一组CRT相互作用的基因。 结果，本发明人检测到编码CRT相互作用多肽（命名为CRTintP）的多核苷酸。 此外，本发明人分离了编码CRTintP的全长cDNA，并显示编码cDNA的基因是新的。 发现应用冷胁迫后，CRT和CRTintP基因的表达显着增加。

公开(公告)号：US20030099998A1

公开(公告)日：2003-05-29

申请号：US10285030

申请日：2002-11-01

Applicant: Japan Science and Technology Corporation

Inventor： Hiroshi Sugiyama ,  Isao Saito ,  Hirokazu Iida

IPC: C12Q001/68 ,  C07H021/04

CPC classification number: G01N33/5011

Abstract: A method for screening the effect of a segment A (chemical species A) on a substance (for example, a cell) containing DNA or RNA by using artificial chemical species. Namely, a method of detecting or identifying the function of a chemical species A on a substance containing DNA or RNA by using one or more chemical species represented by the following general formula (I) which are capable of recognizing a DNA base sequence; a kit therefor; and a plate to be used therein: B-L-A (I) wherein B represents a chemical structure containing an non-natural base capable of recognizing a DNA base sequence; A represents a chemical structure having an interaction with DNA; and L represents a linker whereby the chemical structures A and B can be linked together.

Abstract translation: 通过使用人造化学物质筛选片段A（化学物质A）对含有DNA或RNA的物质（例如细胞）的影响的方法。 即，通过使用能够识别DNA碱基序列的由以下通式（I）表示的一种或多种化学物质来检测或鉴定含有DNA或RNA的物质上的化学物质A的功能的方法; 一套工具; 和其中使用的板：B-L-A（I）其中B表示含有能够识别DNA碱基序列的非天然碱基的化学结构; A表示与DNA相互作用的化学结构; L表示连接物，化学结构A和B可以连接在一起。

公开(公告)号：US20030096947A1

公开(公告)日：2003-05-22

申请号：US10339513

申请日：2003-01-09

Applicant: Japan Science and Technology Corporation, Japan

Inventor： Hitoshi Endou ,  Takashi Sekine ,  Seok Ho Cha

IPC: C07K014/47 ,  C12Q001/68 ,  C07H021/04 ,  C12P021/02 ,  C12N005/06

CPC classification number: C07K14/47 ,  C07K14/705 ,  C12Q1/6876 ,  C12Q2600/158

Abstract: A novel organic anion transporter gene participating in organic anion transport in the placenta; and an organic anion transporter which is a polypeptide encoded by the gene. A placental organic anion transporter OAT4, more particularly, a placental organic anion transporter OAT4 having the amino acid sequence represented by SEQ ID NO:2 or an amino acid sequence derived therefrom by deletion, substitution or addition of a part of the amino acids thereof. A nucleic acid (preferably DNA) having a base sequence encoding the placental organic anion transporter OAT4 or a base sequence hybridizable therewith under stringent conditions.

Abstract translation: 参与胎盘中有机阴离子转运的新型有机阴离子转运蛋白基因; 和由该基因编码的多肽的有机阴离子转运蛋白。 胎盘有机阴离子转运蛋白OAT4，更特别是具有由SEQ ID NO：2表示的氨基酸序列的胎盘有机阴离子转运蛋白OAT4或其部分氨基酸的缺失，取代或添加导致的氨基酸序列。 具有编码胎盘有机阴离子转运蛋白OAT4的碱基序列或在严格条件下可与其杂交的碱基序列的核酸（优选DNA）。