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    MICROFLUIDIC DETECTOR
    31.
    发明申请
    MICROFLUIDIC DETECTOR 审中-公开
    微流体检测器

    公开(公告)号：WO2008060449A2

    公开(公告)日：2008-05-22

    申请号：PCT/US2007023595

    申请日：2007-11-09

    Applicant: HARVARD COLLEGE ,  BUTTE MANISH J ,  WONG AMY P ,  SHARPE ARLENE H ,  WHITESIDES GEORGE M

    Inventor： BUTTE MANISH J ,  WONG AMY P ,  SHARPE ARLENE H ,  WHITESIDES GEORGE M

    IPC: G01N33/543 ,  G01N33/569

    CPC classification number: G01N33/56972 ,  G01N33/54373 ,  G01N2333/16 ,  G01N2333/70503 ,  G01N2800/24

    Abstract: Articles and methods for determining an analyte indicative of a disease condition are provided. In some embodiments, articles and methods described herein can be used for determining a presence, qualitatively or quantitatively, of a component, such as a particular type of cell, in a fluid sample. In one particular embodiment, a low-cost microfluidic system for rapid detection of T cells is provided. The microfluidic system may use immobilized antibodies and adhesion molecules in a channel to capture T cells from a fluid sample such as a small volume of blood. The captured T cells may be labeled with a metal colloid (e.g., gold nanoparticles) using an antibody specific for the T Cell Receptor (TCR), and metallic silver can be catalytically precipitated onto the cells. The number of T cells captured can be counted and may indicate a disease condition of a patient such as severe combined immune deficiency or human immunodeficiency virus.

    Abstract translation: 提供了用于确定指示疾病状况的分析物的文章和方法。 在一些实施方案中，本文描述的制品和方法可以用于确定流体样品中组分（例如特定类型的细胞）的定性或定量存在。 在一个特定实施例中，提供了用于快速检测T细胞的低成本微流体系统。 微流体系统可以在通道中使用固定的抗体和粘附分子以从流体样本（例如小体积血液）捕获T细胞。 捕获的T细胞可以使用对T细胞受体（TCR）特异性的抗体用金属胶体（例如金纳米颗粒）标记，并且可以将金属银催化沉淀到细胞上。 捕获的T细胞的数量可以被计数，并且可以指示患者的疾病状况，例如严重的联合免疫缺陷或人类免疫缺陷病毒。

    MICROSYSTEM SPECTROSCOPY
    32.
    发明申请
    MICROSYSTEM SPECTROSCOPY 审中-公开
    微结构光谱

    公开(公告)号：WO2006125176A1

    公开(公告)日：2006-11-23

    申请号：PCT/US2006/019533

    申请日：2006-05-19

    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  DAMEAN, Nicolae ,  SIA, Samuel, K. ,  LINDER, Vincent ,  NAROVLYANSKY, Max ,  WHITESIDES, George, M.

    Inventor： DAMEAN, Nicolae ,  SIA, Samuel, K. ,  LINDER, Vincent ,  NAROVLYANSKY, Max ,  WHITESIDES, George, M.

    IPC: G01N33/543 ,  B01L3/00

    CPC classification number: G01N21/255 ,  B01L3/5027 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0256 ,  G01N21/05 ,  G01N2021/0346 ,  G01N2201/0638 ,  G02B21/0032

    Abstract: The present invention generally relates to systems and methods for acquiring multiple spectra from a sample, for example, a microfluidic sample. In some aspects of the invention, spectra from a series of locations within a sample are recorded and analyzed, and optionally correlated with an image of the sample. The spectra can be obtained by passing a plurality of light beams through a sample, e.g., using an array of microlenses, diffracting the light beams passing through the sample with a transmission grating, before or after passing the light through the sample, and detecting the resulting light, e.g., as an image. The light beams may pass through the sample before or after passing through the transmission grating. The image can appear as an array of discrete spectra, where each spectrum is associated with a region of the sample where a light beam passed through the sample. The spectra can be analyzed to determine information about the region where the light beam passed through the sample. In some cases, the array of microlenses may be movable and/or replaceable with a second array of microlenses. Also, in some instances, the transmission grating may be movable and/or replaceable with a second transmission grating.

    Abstract translation: 本发明一般涉及用于从样品例如微流体样品获取多个光谱的系统和方法。 在本发明的一些方面，记录和分析来自样品中的一系列位置的光谱，并且可选地与样品的图像相关。 可以通过使多个光束通过样品获得光谱，例如使用微透镜阵列，在通过样品之前或之后通过透射光栅衍射穿过样品的光束，并且检测 产生光，例如，作为图像。 光束可以在通过透射光栅之前或之后通过样品。 该图像可以显示为离散光谱阵列，其中每个光谱与光束通过样品的样品区域相关联。 可以分析光谱以确定关于光束通过样品的区域的信息。 在一些情况下，微透镜阵列可以是可移动的和/或可替代的第二阵列的微透镜。 此外，在一些情况下，透射光栅可以是可移动的和/或可以用第二透射光栅代替。

    SELECTIVE DEPOSITION OF MATERIALS ON CONTOURED SURFACES
    36.
    发明申请
    SELECTIVE DEPOSITION OF MATERIALS ON CONTOURED SURFACES 审中-公开
    选择性沉积在表面上的材料

    公开(公告)号：WO2002086452A2

    公开(公告)日：2002-10-31

    申请号：PCT/US2002/009149

    申请日：2002-03-25

    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  CHILDREN'S MEDICAL CENTER CORPORATION ,  OSTUNI, Emanuele ,  CHEN, Christopher, S. ,  INGBER, Donald, E. ,  WHITESIDES, George, M.

    Inventor： OSTUNI, Emanuele ,  CHEN, Christopher, S. ,  INGBER, Donald, E. ,  WHITESIDES, George, M.

    IPC: G01N

    CPC classification number: B01L3/5085 ,  B01J2219/00317 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00743 ,  B01L3/5088 ,  C12N11/08 ,  Y10S530/812 ,  Y10S530/815

    Abstract: The present invention is directed to a method of patterning materials, such as proteins, on a contoured surface by depositing them onto protrusions on the surface and to a cell containment device that may be constructed by this method. In one embodiment, the present invention is directed to a method of selectively depositing a material on a substrate including a contoured surface including a protrusion and a recess. The method includes applying a first fluid to the contoured surface of the substrate and allowing the first fluid to distribute across a portion of the contoured surface such that the first fluid contacts the protrusion and not the recess. The method also includes allowing a first material to deposit on the substrate where the substrate is in contact with the first fluid. Optionally, this method may further include applying a second fluid to the contoured surface of the substrate, allowing the second fluid to distribute across a portion of the contoured surface such that the second fluid contacts the recess, and allowing a second material to deposit on the substrate where the substrate is in contact with the second fluid. Optionally, the method may still further include applying a third fluid to the contoured surface of the substrate, allowing the third fluid to distribute across a portion of the contoured surface, and allowing a third material with an affinity for one of the first material and the second material to deposit on the substrate only where the one of the first material and the second material is deposited. In one embodiment, of this method the first material is a cytophobic material, the second material is a cytophilic material and the third material is a cell. In another embodiment, the present invention is directed to a cell containment device, including a substrate having a contoured surface, a recess in the surface of the substrate, cytophobic material connected to the surface of the substrate outside the recess and a cytophilic material connected to the recess.

    Abstract translation: 本发明涉及通过将材料沉积在表面上的突起上以及可以通过该方法构建的细胞容纳装置，在轮廓表面上图案化材料（例如蛋白质）的方法。 在一个实施例中，本发明涉及一种选择性地将材料沉积在包括突起和凹陷的轮廓表面的基底上的方法。 该方法包括将第一流体施加到基底的轮廓表面，并允许第一流体分布在轮廓表面的一部分上，使得第一流体接触突出体而不是凹部。 该方法还包括允许第一材料沉积在衬底上，其中衬底与第一流体接触。 可选地，该方法还可以包括将第二流体施加到基底的轮廓表面，允许第二流体跨过轮廓表面的一部分分布，使得第二流体接触凹部，并允许第二材料沉积在 衬底，其中衬底与第二流体接触。 可选地，该方法还可以进一步包括将第三流体施加到基底的轮廓表面，允许第三流体跨越轮廓表面的一部分分布，并允许具有与第一材料和 第二材料仅沉积在第一材料和第二材料之一上沉积在基底上。 在一个实施方案中，该方法的第一种材料是细胞免疫材料，第二种材料是嗜细胞性材料，第三种材料是细胞。 在另一个实施方案中，本发明涉及一种细胞容纳装置，包括具有轮廓表面的基底，基底表面中的凹槽，连接到凹部外部的基底表面的细胞免疫材料和连接到 凹槽

    ELECTRIC MICROCONTACT PRINTING METHOD AND APPARATUS
    37.
    发明申请
    ELECTRIC MICROCONTACT PRINTING METHOD AND APPARATUS 审中-公开
    电动微型打印方法和装置

    公开(公告)号：WO0203142A2

    公开(公告)日：2002-01-10

    申请号：PCT/US0121151

    申请日：2001-07-02

    Applicant: HARVARD COLLEGE ,  JACOBS HEIKO O ,  WHITESIDES GEORGE M

    Inventor： JACOBS HEIKO O ,  WHITESIDES GEORGE M

    IPC: G03F7/00 ,  G03G5/02

    CPC classification number: G03G5/02 ,  B82Y10/00 ,  B82Y30/00 ,  B82Y40/00 ,  G03F7/0002

    Abstract: A method and apparatus for providing electric microcontact printing is provided. A stamp is brought into contact with the surface of a substrate to provide high resolution features. Aspects of the invention may be used for data storage, microcontact printing, and for other applications requiring high resolution pattern transfer.

    Abstract translation: 提供了一种用于提供电微接触印刷的方法和装置。 使印模与基板的表面接触以提供高分辨率特征。 本发明的各方面可用于数据存储，微接触印刷以及需要高分辨率图案转印的其它应用。

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