公开(公告)号：KR1020100060161A

公开(公告)日：2010-06-07

申请号：KR1020080118638

申请日：2008-11-27

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 강태선 ,  우선욱 ,  박현주 ,  이윤숙 ,  민홍기

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q2600/156 ,  C12Q2600/172

Abstract: PURPOSE: A method for analyzing the haplotype of CYP2C9 is provided to confirm SNP(single nucleotide polymorphism) and to lower the risk of failures. CONSTITUTION: A method for analyzing CYP2C9 haplotype comprises: a step of obtaining a promoter and exon 7 encoding CYP2C9 from a genome DNA and determining a nucleotide sequence; a step of detecting the presence of SNP in each sequence; and a step of identifying the haplotype of the detected SNP.

Abstract translation: 目的：提供CYP2C9单体型分析方法，以确认SNP（单核苷酸多态性）并降低失败风险。 构成：分析CYP2C9单体型的方法包括：从基因组DNA获得编码CYP2C9的启动子和外显子7的步骤，并测定核苷酸序列; 检测每个序列中SNP的存在的步骤; 以及鉴定检测到的SNP的单体型的步骤。

公开(公告)号：KR1020080105586A

公开(公告)日：2008-12-04

申请号：KR1020070053354

申请日：2007-05-31

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 진선경 ,  강태선 ,  노재숙 ,  이윤숙 ,  박현주 ,  이종은 ,  김주일 ,  최수영

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/172

Abstract: A haplotype analysis kit for the gene coding CYP2C19, and a method for analyzing the haplotype of the gene coding CYP2C19 by using the kit are provided to analyze the genetic polymorphism of CYP2C19 more easily. A haplotype analysis kit for the gene coding CYP2C19 comprises a primer for PCR amplification having a base sequence selected from the group consisting of the sequence numbers 1 to 44, and a primer for base sequence determination having a base sequence selected from the group consisting of the sequence numbers 45-88; can analyze SNP (single nucleotide polymorphism) having the genetic variation selected from -888T>G, -97T>C, P33P, IVS1-47G>A, IVS2+147A>G, W212X, P227P, IVS5-51C>G, V330V, IVS7-106T>C and G417G, in the CYP2C19 of a cytochrome P450-based enzyme or the gene which codes the CYP2C19 and has a base sequence represented by the sequence number 89; and can analyze the haplotype group selected from H1 to H14 specified in the table of the gene coding CYP2C19 having a base sequence represented by the sequence number 89.

Abstract translation: 提供了一种编码CYP2C19基因的单体型分析试剂盒，通过使用该试剂盒分析编码CYP2C19基因的单倍型的方法，更容易分析CYP2C19的遗传多态性。 用于编码CYP2C19的基因的单元型分析试剂盒包括用于PCR扩增的引物，其具有选自序列号1至44的碱基序列，和用于碱基序列测定的引物，其具有选自以下的碱基序列： 序列号45-88; 可以分析具有遗传变异选自-888T> G，-97T> C，P33P，IVS1-47G> A，IVS2 + 147A> G，W212X，P227P，IVS5-51C> G，V330V的SNP（单核苷酸多态性） IVS7-106T> C和G417G，在基于细胞色素P450的酶的CYP2C19中或编码CYP2C19并具有由序列号89表示的碱基序列的基因; 并且可以分析选自具有由序列号89表示的碱基序列的编码CYP2C19的基因的表中指定的H1至H14的单倍型组。