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公开(公告)号:KR1020080103255A
公开(公告)日:2008-11-27
申请号:KR1020070050318
申请日:2007-05-23
Applicant: 김철욱 , 경남과학기술대학교 산학협력단
IPC: C12Q1/68
CPC classification number: C12Q1/683 , C12Q2565/125
Abstract: A method for determining economic traits of pig is provided to determine the economic traits quickly, simply and exactly using an RFLP and to contribute scientific farming to hog raising farms. A method for determining economic traits of pig comprises steps of: amplifying Growth Hormone Releasing Hormone gene fragment of the pig using a primer for PCR of one set consisting of a sequence number 1 and a sequence number 2; and confirming an AA type from AluI Restriction Fragment Length Polymorphism of a pig GHRH gene after being processed by a limitation enzyme AluI and electrophoresed.
Abstract translation: 提供了一种确定猪经济性状的方法,通过RFLP快速,简单,准确地确定经济性状,为养殖农场提供科学养殖。 用于确定猪经济性状的方法包括以下步骤:使用由序列号1和序列号2组成的一组的PCR引物扩增猪的生长激素释放激素基因片段; 并通过限制酶AluI处理后的猪GHRH基因的AluI限制性片段长度多态性确认AA型并进行电泳。