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    돼지의 성장, 육질 및 번식 형질과 관련된 유전자를 조기에진단할 수 있는 ｃＤＮＡ ｃｈｉｐ 과 그 개발방법 및상기 칩을 이용한 성장, 육질 및 번식 형질이 우수한 종돈선별방법
    1.
    发明公开
    돼지의 성장, 육질 및 번식 형질과 관련된 유전자를 조기에진단할 수 있는 ｃＤＮＡ ｃｈｉｐ 과 그 개발방법 및상기 칩을 이용한 성장, 육질 및 번식 형질이 우수한 종돈선별방법 无效
    诊断皮下生长发育的CDNA芯片，血清，出生数目相关基因及其发展方法，诊断方法

    公开(公告)号：KR1020090065412A

    公开(公告)日：2009-06-22

    申请号：KR1020080046982

    申请日：2008-05-21

    Applicant: 김철욱 ,  경남과학기술대학교 산학협력단

    Inventor： 김철욱 ,  조은석 ,  권은정 ,  정원용 ,  박다혜

    IPC: C12Q1/68

    CPC classification number: C12Q1/6888 ,  C12Q2600/124

    Abstract: A cDNA chip which early diagnoses a gene related to the growth, meat quality and breeding character of pig is provided to analyze the massive gene source of pig at once. A method for producing a cDNA chip which diagnoses a gene related to the growth, meat quality and breeding character of pig comprises: a step of preparing tissue sample from a tissue and blood of pig; a step of isolating RNA from the tissue sample; a step of producing cDNA library with 4,172 ESTs cloned from RNA; a step of producing a 4,200 cDNA chips containing 28 gene of beta-actin(Acc. No. DQ845171) and 18S(Acc. No. AF102857); and a step of mixing 254 gene having the significance in growth, meat quality and breeding character and 10 positive control genes to produce 264 cDNA chips.

    Abstract translation: 提供早期诊断与猪的生长，肉质和繁殖特性相关的基因的cDNA芯片，用于一次性分析猪的大量基因来源。 一种用于诊断与猪的生长，肉质和育种特性相关的基因的cDNA芯片的方法，包括：从猪的组织和血液制备组织样品的步骤; 从组织样品中分离RNA的步骤; 从RNA中克隆出4,172个EST的cDNA文库生成步骤; 生成含有β-肌动蛋白28基因（Acc。No.DD845171）和18S（Acc.No.AF102857）的4,200个cDNA芯片的步骤; 以及混合具有生长，肉质和育种特征的10种阳性对照基因的254基因的混合物的步骤，生成264个cDNA片段。

    산청흑돼지 유래의 콜라겐 타입 Ｉ α­１ 유전자의발현량을 정량적으로 측정하여 돼지의 육질을 평가하는방법
    2.
    发明公开
    산청흑돼지 유래의 콜라겐 타입 Ｉ α­１ 유전자의발현량을 정량적으로 측정하여 돼지의 육질을 평가하는방법 无效
    通过测定胶原I型ALPHA的表达水平测定PIG的评价方法;从SANCHEONG BERKSHIRE衍生的-1基因

    公开(公告)号：KR1020080074298A

    公开(公告)日：2008-08-13

    申请号：KR1020070013128

    申请日：2007-02-08

    Applicant: 김철욱 ,  경남과학기술대학교 산학협력단

    Inventor： 김철욱 ,  서상식 ,  조은석 ,  정원용 ,  권은정 ,  박다혜 ,  조광근 ,  정기화

    IPC: C12Q1/68

    CPC classification number: C12Q1/6851 ,  C12Q2600/124

    Abstract: A method for evaluating the quality of meat of a pig is provided to evaluate the pig meat quality conveniently and accurately by measuring an expression level of a novel collagen type I alpha-1 gene derived from Sancheong berkshire. To evaluate the quality of meat of a pig, an expression level of a collagen type I alpha-1 gene derived from Sancheong berkshire and consisting of SEQ ID : NO. 1 is measured quantitatively through RT-PCR using an oligonucleotide consisting of SEQ ID : NOs. 3 and 4 as a primer or through northern blotting using a probe consisting of SEQ ID : NO. 1.

    Abstract translation: 提供了一种评价猪肉质量的方法，通过测量源自Sancheong berkshire的新型胶原I型α-1基因的表达水平，方便，准确地评价猪肉质量。 为了评价猪的质量，来自Sancheong berkshire的I型胶原蛋白的表达水平由SEQ ID NO： 1通过使用由SEQ ID NO：构成的寡核苷酸的RT-PCR进行定量测定。 3和4作为引物或通过Northern印迹，使用由SEQ ID NO： 1。

    ＲＦＬＰ를 이용한 돼지의 경제형질 판단 방법
    3.
    发明公开
    ＲＦＬＰ를 이용한 돼지의 경제형질 판단 방법 无效
    用于确定使用RFLP的PIG的经济旅行的方法

    公开(公告)号：KR1020080103255A

    公开(公告)日：2008-11-27

    申请号：KR1020070050318

    申请日：2007-05-23

    Applicant: 김철욱 ,  경남과학기술대학교 산학협력단

    Inventor： 김철욱 ,  권은정 ,  정원용 ,  박다혜 ,  조은석 ,  조광근 ,  정기화 ,  장규태 ,  홍성광

    IPC: C12Q1/68

    CPC classification number: C12Q1/683 ,  C12Q2565/125

    Abstract: A method for determining economic traits of pig is provided to determine the economic traits quickly, simply and exactly using an RFLP and to contribute scientific farming to hog raising farms. A method for determining economic traits of pig comprises steps of: amplifying Growth Hormone Releasing Hormone gene fragment of the pig using a primer for PCR of one set consisting of a sequence number 1 and a sequence number 2; and confirming an AA type from AluI Restriction Fragment Length Polymorphism of a pig GHRH gene after being processed by a limitation enzyme AluI and electrophoresed.

    Abstract translation: 提供了一种确定猪经济性状的方法，通过RFLP快速，简单，准确地确定经济性状，为养殖农场提供科学养殖。 用于确定猪经济性状的方法包括以下步骤：使用由序列号1和序列号2组成的一组的PCR引物扩增猪的生长激素释放激素基因片段; 并通过限制酶AluI处理后的猪GHRH基因的AluI限制性片段长度多态性确认AA型并进行电泳。

    조직별, 품종별, 성장단계별 유전자 발현양상 분석을 통하여 확보한 유전자를 이용하여 성장 또는 육질이 우수한 종돈을 조기에 선발하는 방법
    5.
    发明公开
    조직별, 품종별, 성장단계별 유전자 발현양상 분석을 통하여 확보한 유전자를 이용하여 성장 또는 육질이 우수한 종돈을 조기에 선발하는 방법 失效
    通过分析部分，育成和生长阶段诊断生长和花期的特殊基因

    公开(公告)号：KR1020090065831A

    公开(公告)日：2009-06-23

    申请号：KR1020070133339

    申请日：2007-12-18

    Applicant: 김철욱 ,  경남과학기술대학교 산학협력단

    Inventor： 김철욱 ,  조은석 ,  권은정 ,  정원용 ,  박다혜 ,  서상식 ,  전양제 ,  이용화 ,  강진훈

    IPC: C12N15/12 ,  C12Q1/68

    CPC classification number: C12Q1/6827 ,  C12Q1/6888 ,  C12Q2600/124

    Abstract: A specific gene for diagnosing pig growth and the diagnosis of meat quality is provided to early select excellent pig species by obtaining the specific gene related to growth and meat quality. A method for obtaining a specific gene for diagnosing growth and meat quality of pig comprises: a step of building a cDNA library using muscle and fat tissue of Sus scrofa, designing and analyzing cDNA microarray; a step of collecting muscle and fat from Sus scrofa and Yorkshire and making tissue sample; a step of cloning a gene from the tissue sample; a step of analyzing full-sequence; a step of isolating total RNA; a step of synthesizing first strand cDNA using superscript II reverse transcriptase; and a step of performing statistical analysis.

    Abstract translation: 通过获得与生长和肉质相关的具体基因，提供了诊断猪生长和肉质诊断的具体基因，以早期选择优良的猪种。 用于获得用于诊断猪的生长和肉质的特定基因的方法包括：使用Sus scrofa的肌肉和脂肪组织构建cDNA文库的步骤，设计和分析cDNA微阵列; 从Sus scrofa和Yorkshire收集肌肉和脂肪并制作组织样本的步骤; 从组织样品中克隆基因的步骤; 分析全序列的步骤; 分离总RNA的步骤; 使用上标II逆转录酶合成第一链cDNA的步骤; 以及执行统计分析的步骤。

    돼지의 육질 검사용 프라이머 및 이를 이용한 돼지의 육질판단 방법
    6.
    发明公开
    돼지의 육질 검사용 프라이머 및 이를 이용한 돼지의 육질판단 방법 无效
    用于测试皮肤感光度的原始样本和使用标准染色剂确定皮肤感光度的方法

    公开(公告)号：KR1020080103267A

    公开(公告)日：2008-11-27

    申请号：KR1020070050352

    申请日：2007-05-23

    Applicant: 김철욱 ,  경남과학기술대학교 산학협력단

    Inventor： 김철욱 ,  권은정 ,  정원용 ,  박다혜 ,  조은석 ,  조광근 ,  정기화 ,  장규태 ,  서상식

    IPC: C12Q1/68

    CPC classification number: C12Q1/683 ,  C12Q1/6844

    Abstract: A primer for testing flesh of pig and a method of testing the flesh of the pig using the same are provided to examin conveniently and exactly the flesh of the pig. A primer for testing flesh of pig comprises a sequence number 1 and a sequence number 2. A method of testing the flesh of the pig comprises measuring an expression amount of the gallectin-1 gene of the pig. An RT-PCR is used by a measurement of the expression amount. The primer consisting of the sequence number 1 and the sequence number 2 is used by the measurement of the expression amount.

    Abstract translation: 提供了用于测试猪肉的底漆和使用其的猪的肉的测试方法，以方便和准确地检查猪的肉。 用于测试猪的肉的引物包含序列号1和序列号2.测试猪的肉的方法包括测量猪的gallectin-1基因的表达量。 通过测量表达量使用RT-PCR。 由序列号1和序列号2构成的引物通过表达量的测定来使用。

    돼지의 조기선발을 위한 신규한 마커와 이를 이용한 평가방법
    7.
    发明公开
    돼지의 조기선발을 위한 신규한 마커와 이를 이용한 평가방법 有权
    SNP标记和评估猪肉的方法

    公开(公告)号：KR1020120127178A

    公开(公告)日：2012-11-21

    申请号：KR1020110141381

    申请日：2011-12-23

    Applicant: 경남과학기술대학교 산학협력단

    Inventor： 김철욱 ,  방우영 ,  조은석 ,  권은정 ,  박다혜 ,  이용화 ,  권슬기

    IPC: C12Q1/68 ,  C12N15/11

    CPC classification number: C12Q1/6827 ,  C12Q1/6888 ,  C12Q2600/124 ,  C12Q2600/156

    Abstract: PURPOSE: A novel SNP marker for early selecting pigs with preferable parts of high content and a method using the same are provided to early select breeding pigs. CONSTITUTION: A method for evaluating economic characters of pigs comprises a step of selecting a pig that SNP locus of serial number 3 is CT, SNP locus of serial number 13 is CT, SNP locus of serial number 15 is AA or GG, SNP locus of serial number 16 is GG, or SNP locus of serial number 17 is GG; and a step of determining the pig which has high quality as one which corresponds to many loci.

    Abstract translation: 目的：提供早期选择高含量部分猪的新型SNP标记物及其使用方法，以提早选择育种猪。 构成：评价猪经济特性的方法包括选择序列号3为SNP SNP为CT的猪，序列号13为SNP的SNP为CT，序列号15的SNP位点为AA或GG，SNP位点为 序列号16是GG，序列号17的SNP位点是GG; 并且将具有高质量的猪确定为对应于许多基因座的步骤。

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