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公开(公告)号：KR101634272B1
公开(公告)日：2016-06-30
申请号：KR1020140077284
申请日：2014-06-24
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85 , A01K67/033 , D01C3/00
Abstract: 본발명은청색형광실크를생산하는형질전환누에에관한것이다. 본발명의청색형광실크를생산하는형질전환누에는, 표지유전자조절프로모터, 표지유전자, 누에유래의피브로인(Fibroin) 프로모터및 해파리(Aequorea macrodactyla) 유래청색형광유전자인 EBFPco가작동가능하게연결된유전자컨스트럭트를포함하는재조합발현벡터를제작하여상기재조합발현벡터로누에를형질전환시킴으로써청색형광실크를생산하는것이특징이다. 본발명에의해, 자연광에서도진한천연의청색을나타내어별도의염색이필요없어친환경적이고경제적이며특정파장의빛을쏘이면어둠속에서영롱한청색을나타내어일반실크에비해훨씬고부가가치의고급패션의류나벽지등의소재로유용하게사용될수 있는청색형광실크가제공된다.
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公开(公告)号：KR101533602B1
公开(公告)日：2015-07-06
申请号：KR1020130072203
申请日：2013-06-24
Applicant: 대한민국(농촌진흥청장)
IPC: A61K35/744 , A61K35/747 , A61K31/739 , A61K31/70 , A61K35/64 , A61K39/39
Abstract: 본발명은누에면역반응유도용조성물및 이에의해면역이유도되어항균활성이부가된누에에관한것이다. 본발명의누에면역반응유도용조성물은단백질이제거된유산균파쇄액추출물을유효성분을포함하는것을특징으로그 제조방법은유산균을배양하는제1단계; 상기배양된유산균을원심분리한후 상등액을제거하여균체를획득한다음, 버퍼를넣어유산균현탁액을제조하는제2단계; 상기유산균현탁액을초음파분해로인해유산균의세포를파쇄하여유산균세포파쇄물을제조하는제3단계; 상기유산균세포파쇄물에부탄올을첨가하여추출한다음원심분리한후, 상등액을제거하여단백질이제거된하등액을얻는제4단계; 상기하등액은 1차동결건조하여동결건조물을제조하는제5단계; 상기동결건조물을물로녹인후, 이를투석한다음 2차동결건조하여단백질이제거된유산균파쇄액추출물을제조하는제 6단계및, 상기단백질이제거된유산균파쇄액추출물을포함하는누에면역반응유도용조성물을제조하는제7단계;를포함하는것이특징이다. 본발명에의해, 누에의체액성면역반응으로생성되는항균성단백질발현을유도하는누에면역반응유도용조성물및 이에의해면역이유도되어항균활성이부가된누에가제공된다.
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公开(公告)号：KR101424079B1
公开(公告)日：2014-08-04
申请号：KR1020100105590
申请日：2010-10-27
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 재조합 대장균을 이용한 파필리오신의 대량 생산방법에 관한 것이다.
본 발명의 재조합 대장균을 이용한 파필리오신의 대량 생산방법은 호랑나비(
Papilio
xuthus ) 유충 유래의 파필리오신 유전자를 갖는 형질전환된 재조합 대장균을 제조하는 단계와, 상기의 형질전환체인 재조합 대장균을 배양한 후, 이를 불용성 재조합 융합단백질로 발현한 후 분리 및 정제하여 파필리오신을 생산하는 단계를 포함하여 구성된다.
본 발명에 의해, 재조합된 파필리오신은 그람음성 세균 및 그람양성 세균 등의 각종 박테리아에 대하여 높은 항균활성을 나타내므로 천연 항생제로 유용하게 이용될 수 있다.-
公开(公告)号：KR1020140057786A
公开(公告)日：2014-05-14
申请号：KR1020120123971
申请日：2012-11-05
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85 , C12N15/12 , A01K67/027
Abstract: The present invention relates to a transgenic silkworm that produces red fluorescent silk. More specifically, a recombinant expression vector which includes a gene construct in which a marker gene regulatory promoter, a marker gene, a silkworm-derived fibroin promoter, and an anemone-derived red fluorescent gene are operatively linked and a silkworm is transformed by the recombinant expression vector so that a transgenic silkworm that produces red fluorescent silk can be produced. The red fluorescent silk that the transgenic silkworm produces represents thin natural red color even in natural light so that a separated coloring process is not needed and the silk is environment-friendly and economical. When a light beam of a particular wavelength is irradiated, the silk represents bright red fluorescent color in the darkness so as to be usefully used as material for high value clothes and fashion or wallpaper compared with general silk.
Abstract translation: 本发明涉及产生红色荧光丝的转基因蚕。 更具体而言，包含基因构建体的重组表达载体，其中标记基因调节启动子，标记基因，蚕衍生的丝心蛋白启动子和来自海葵的红色荧光基因可操作地连接，并且通过重组体转化蚕 表达载体，从而可以产生产生红色荧光丝的转基因蚕。 转基因蚕产生的红色荧光丝即使在自然光下也能表现出稀薄的天然红色，因此不需要分离的着色过程，丝绸环保，经济。 当照射特定波长的光束时，丝绸在黑暗中代表明亮的红色荧光颜色，以便与普通丝绸相比有用地用作高价值衣服和时尚或壁纸的材料。
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公开(公告)号：KR1020130141958A
公开(公告)日：2013-12-27
申请号：KR1020120065118
申请日：2012-06-18
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a transgenic silkworm having hSCF recombinant protein. A method for preparing the transgenic silkworm having hSCF recombinant protein of the present invention comprises the steps of: introducing a dHsp70 promoter and a 3xP3 promoter to a transfer vector in order to prepare an expression vector for silkworm transformation; and micro-injecting the expression vector for the silkworm transformation into a silkworm egg and preparing a transduced transgenic silkworm. By the present invention, the transgenic silkworm, which is capable of mass producing stem cell factors (hSCF) which are useful to the human body at low costs, is provided. [Reference numerals] (1) Not transformed silkworm larva after 5 days;(2) Transformed silkworm larva after 5 days
Abstract translation: 本发明涉及具有hSCF重组蛋白的转基因蚕。 制备本发明具有hSCF重组蛋白的转基因蚕的方法包括以下步骤：将dHsp70启动子和3xP3启动子引入转移载体，以制备用于蚕转化的表达载体; 并将蚕转化的表达载体微注射到蚕卵中并制备转导的转基因蚕。 通过本发明，提供了能够以低成本大量生产对人体有用的干细胞因子（hSCF）的转基因蚕。 [参考数字]（1）5天后未转化的蚕幼虫;（2）5天后转化的蚕幼虫
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Patent Agency Ranking36.发明公开호랑나비 유충에서 분리한 항균펩타이드 파필리오신2의 유전자, 이를 이용한 재조합 항균펩타이드 파필리오신2 및 이의 대량생산방법 无效抗生素PEPTIDE PAPILIOCIN2的基因分离自黑曲霉BARTERFLY的LARVAE，使用其的重组抗生素肽PAPILIOCIN2及其生产的大量方法
公开(公告)号：KR1020130007783A
公开(公告)日：2013-01-21
申请号：KR1020110068317
申请日：2011-07-11
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A papiliocin 2 gene isolated from a Papilio xuthus larva, an antipeptide using the same, and a method for producing a large amount of recombinant papiliocin 2 are provided to ensure antibacterial activity against gram-negative bacteria, Candida albicans, and resistant bacteria(MEREC). CONSTITUTION: A gene of antibacterial peptide papiliocin 2 is denoted by sequence number 7 and is isolated from Papilio xuthus larva. A recombinant antibacterial peptide papiliocin 2 is denoted by sequence number 9. A recombinant expression vector, pET32-Papiliocin2 contains the recombinant antibacterial peptide papiliocin 2. A recombinant E.col BL21(DE3)-(pET32-Papiliocin2) is formed by transforming with the recombinant expression vector pET32-Papiliocin2. A method for producing a large amount of the recombinant antibacterial peptide pailiocin 2 comprises: a step of preparing the recombinant antibacterial peptide papiliocin 2(sequence number 9); a step of preparing the recombinant E.coli by the recombinant expression vector containing the recombinant antibacterial peptide papiliocin 2; and a step of culturing the recombinant E.coli and isolating and purifying a water soluble recombinant fusion protein.
Abstract translation: 目的：提供从Papilio xuthus幼虫分离的乳糜蛋白酶2基因，使用其的抗肽，以及用于产生大量重组体生物素酶2的方法，以确保对革兰氏阴性细菌，白色念珠菌和抗性细菌的抗菌活性（ MEREC）。 构成：抗菌肽乳木球蛋白2的基因由序列号7表示，并从Papilio xuthus幼虫中分离。 重组抗菌肽乳木球蛋白2以序列号9表示。重组表达载体pET32-青霉素2含有重组抗菌肽乳糖酶2.重组大肠杆菌BL21（DE3） - （pET32-青霉素2）通过用 重组表达载体pET32-Papiliocin2。 一种生产大量重组抗菌肽潘立替康2的方法，包括：制备重组抗菌肽乳链球蛋白2（序列号9）的步骤; 通过重组表达载体制备重组大肠杆菌的步骤，该重组表达载体含有重组抗菌肽乳木球蛋白2; 以及培养重组大肠杆菌并分离和纯化水溶性重组融合蛋白的步骤。
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37.发明公开누에 형질전환용 열충격단백질 70 프로모터, 이의 증폭용 프라이머, 이 프로모터를 포함하는 발현벡터, 이 발현벡터로 형질전환된 누에 형질전환체 및 그 제조방법 有权热冲击蛋白70促进剂用于丝光纤变换，用于放大的引导器，包含促销器的表达向量，用表达式向量变换的丝绸变换器及其生产方法
公开(公告)号：KR1020130006151A
公开(公告)日：2013-01-16
申请号：KR1020110068021
申请日：2011-07-08
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A HSP70 promoter for transforming silkworm, a primer for amplifying the same, and an expression vector containing the promoter are provided to express green fluorescent genes in silkworm eggs and larvae, and to early determine transformation. CONSTITUTION: A HSP70 promoter for transforming silkworm is denoted by sequence number 1, and is isolated in a silkworm. A primer for amplifying the promoter has sequences of sequence numbers 2 and 3. An expression vector for transforming silkworm contains the HSP70 promoter. A method for preparing a silkworm transformant comprises: a step of treating a dormant silkworm egg with hydrochloric acid at 25 deg. C for 1 hour; a step of transducing green fluorescent gene at downstream of the HSP70 promoter(sequence number 1) and cloning fused vector to prepare the expression vector; and a step of microinjecting the expression vector to the silkworm egg.
Abstract translation: 目的：提供用于转化蚕的HSP70启动子，其扩增引物和含有启动子的表达载体，以在蚕卵和幼虫中表达绿色荧光基因，并早期确定转化。 构成：用于转化蚕的HSP70启动子由序列号1表示，并在蚕中分离。 用于扩增启动子的引物具有序列号2和3的序列。转化蚕的表达载体含有HSP70启动子。 制备蚕转化体的方法包括：用25℃的盐酸处理休眠蚕卵的步骤。 C 1小时; 在HSP70启动子（序列号1）的下游转染绿色荧光基因并克隆融合载体以制备表达载体的步骤; 以及向蚕卵显微注射表达载体的步骤。
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38.发明公开녹색 형광실크를 생산하기 위한 형질전환용 누에알, 그 누에알로부터 제조된 누에 형질전환체 및 이를 통해 생산된 형질전환 누에 有权用于生产绿色荧光丝的转基丝丝蛋白，使用其制造的丝素变压器和使用其生产的转基因丝
公开(公告)号：KR1020120124644A
公开(公告)日：2012-11-14
申请号：KR1020110042423
申请日：2011-05-04
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A silkworm egg for transformation for producing green fluorescence silk and a transformant from the same are provided to stably and cheaply produce useful materials. CONSTITUTION: A silkworm egg for transformation is prepared by acid treatment of an egg of dormant state at 25 Deg. C. for one hour. A method for preparing a silkworm transformant comprises: a step of preparing the silkworm egg; a step of cloning a cassette vector to a basic vector in which a red fluorescent gene is introduced at 3XP promoter downstream and preparing a delivery system for silkworm transformation; and a step of microinjection of the delivery system to the silkworm egg. [Reference numerals] (AA) Hatching rate(%); (BB, DD) Untreated; (CC, EE) Micro-injection
Abstract translation: 目的：提供用于生产绿色荧光丝的转化的蚕卵及其转化体，以稳定且廉价地生产有用的材料。 构成：通过酸处理休眠状态的鸡蛋25度来制备用于转化的蚕卵。 C.一个小时。 一种制备蚕转化体的方法，包括：制备蚕卵的步骤; 将盒载体克隆到其中在下游3XP启动子处引入红色荧光基因并制备用于蚕转化的递送系统的碱性载体的步骤; 以及向蚕卵显微注射输送系统的步骤。 （标号）（AA）孵化率（％） （BB，DD）未处理; （CC，EE）微量注射
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公开(公告)号：KR101634275B1
公开(公告)日：2016-06-29
申请号：KR1020130121054
申请日：2013-10-11
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85 , C12N15/12 , C12N5/10 , A01K67/033
Abstract: 본발명은청색형광실크를생산하는형질전환누에에관한것으로, 보다상세하게는표지유전자조절프로모터, 표지유전자, 누에유래의피브로인(Fibroin) 프로모터및 해파리(Aequorea macrodactyla) 유래청색형광유전자가작동가능하게연결된유전자컨스트럭트를포함하는재조합발현벡터를제작하여상기재조합발현벡터로누에를형질전환시킴으로써청색형광실크를생산하는형질전환누에를제조할수 있으며, 상기형질전환누에가생산하는청색형광실크는자연광에서도엷은천연의청색을나타내어별도의염색이필요없어친환경적이고경제적이며특정파장의빛을쏘이면어둠속에서영롱한청색을나타내어일반실크에비해훨씬고부가가치의고급패션의류나벽지등의소재로유용하게사용될수 있다.
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公开(公告)号：KR1020160000523A
公开(公告)日：2016-01-05
申请号：KR1020140077265
申请日：2014-06-24
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/10 , C12N15/86 , A01K67/033
Abstract: 본발명은누에후부실샘특이적으로발현하는유전자(ACP-16), 서열번호 3의핵산서열을가지는프로모터, 및이의용도에관한것이다. 본발명에따른신규유전자(ACP-16)의프로모터및 이를포함하는벡터는누에후부실샘특이적유전자발현유도능을가지기때문에, 이를사용하여형질전환된누에는외래단백질이포함된견사견또는견사를생산할수 있어, 외래단백질정제과정의편리성및 색소유전자발현에의한견사개량이가능하므로, 산업적으로매우유용하다.
Abstract translation: 本发明涉及在后丝腺中特异性表达的基因（ACP-16），涉及具有SEQ ID NO：3的核酸序列的启动子及其用途。 根据本发明，新基因（ACP-16）的启动子及其含有的载体具有诱导后丝腺特异性基因表达的能力。 因此，转化后的家蚕在工业上可以使用它们，能够生产含有外来蛋白质的丝线或丝腺，并通过色素基因表达改善丝线，并且方便了外来蛋白质的精制。
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