公开(公告)号：KR1020090108236A

公开(公告)日：2009-10-15

申请号：KR1020080033551

申请日：2008-04-11

Applicant: 한국표준과학연구원

Inventor： 김숙경

IPC: C12N15/52 ,  C12N15/09 ,  C12N15/64

Abstract: PURPOSE: A method for producing proteins using a signal sequence of chorismate mutase gene from yersinia pestis, and a recombinant expression vector therefor are provided to efficiently transport YPCM to a gap between cells. CONSTITUTION: A method for producing proteins using a signal sequence of chorismate mutase gene from yersinia pestis comprises the steps of: expressing a recombinant expression vector and transporting the recombinant expression vector to a gap inside cells; and separating the transported protein from the gap inside cells. The recombinant expression vector includes a signal sequence from a gene. The gene is the chorismate mutase gene from yersinia pestis gene which connected gene coding a protein.

Abstract translation: 目的：使用来自鼠疫耶尔森氏菌的chorismate mutase基因的信号序列产生蛋白质的方法及其重组表达载体用于有效地将YPCM转运至细胞间隙。 构成：使用鼠疫耶尔森氏菌的chorismate mutase基因的信号序列生产蛋白质的方法包括以下步骤：表达重组表达载体并将重组表达载体转运至细胞内的间隙; 并将转运的蛋白质与细胞内的间隙分离。 重组表达载体包含来自基因的信号序列。 该基因是连接基因编码蛋白质的鼠疫耶尔森氏菌基因的chorismate mutase基因。