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公开(公告)号:KR1020110013726A
公开(公告)日:2011-02-10
申请号:KR1020090071330
申请日:2009-08-03
Applicant: 한국화학연구원
IPC: C07K14/00
Abstract: PURPOSE: A thermally stable CiP(Coprinus cinereus peroxidase) mutant protein and a DNA encoding the same are provided to ensure high thermal stability and to be used in various industries. CONSTITUTION: A thermally stable CiP(Coprinus cinereus peroxidase) mutant protein has 323th amino acid of sequence number 1, in which serine is substituted with tyrosine; 328th amino acid in which glutamic acid is substituted with aspartic acid; or both thereof. A DNA encoding CiP mutant protein has 967-969th bases of sequence number 5, which are substituted with a codon encoding tyrosine and has a base sequence of sequence number 6.
Abstract translation: 目的:提供热稳定的CiP(鬼马菌过氧化物酶)突变蛋白和编码它的DNA,以确保高热稳定性并用于各种行业。 构成:热稳定的CiP(鬼马菌过氧化物酶)突变蛋白具有序列号1的323位氨基酸,其中丝氨酸被酪氨酸取代; 谷氨酸被天冬氨酸取代的第328位氨基酸; 或其两者。 编码CiP突变蛋白的DNA具有序列号5的967-969位碱基,其被编码酪氨酸的密码子取代并具有序列号6的碱基序列。