公开(公告)号：EP0227795A1

公开(公告)日：1987-07-08

申请号：EP86904513.0

申请日：1986-06-13

Applicant: Amgen ,  ABBOTT LABORATORIES

Inventor： SNITMAN, David, L. ,  STROUPE, Stephen, D.

IPC: G01N33 ,  C07H21 ,  C12N15 ,  C12Q1

CPC classification number: G01N33/54353 ,  C12Q1/6804 ,  C12Q1/6813 ,  C12Q1/705 ,  G01N33/532

Abstract: Un procédé et un kit d'isolement et de détection quantitative dans une solution d'une séquence sélectionnée d'acide nucléique cible comprennent deux agents d'investigation. Un premier agent d'investigation est complémentaire d'une partie de la cible et est attaché de manière covalente à un premier complexant (un antigène ou un anticorps, par exemple). Le deuxième agent d'investigation est complémentaire d'une partie différente de la cible et est associé à un groupe rapporteur. Apès l'hybridation de la cible et des deux agents d'investigation dans la solution, on utilise un support solide enduit d'un deuxième complexant (un anticorps ou un antigène correspondants, par exemple) capable de se lier au premier complexant sur le premier agent d'investigation afin d'immobiliser le composé hybride de cible et d'agent d'investigation. Le premier agent d'investigation utilisé peut appartenir à plusieurs types différents. Chaque type s'attache au même genre de complexant mais comprend une séquence d'acides nucléiques complémentaire d'une partie différente de la cible.

公开(公告)号：EP1144449B1

公开(公告)日：2010-04-07

申请号：EP00903373.9

申请日：2000-01-21

Applicant: ABBOTT LABORATORIES

Inventor： BILLING-MEDEL, Patricia, A. ,  COHEN, Maurice ,  COLPITTS, Tracey, L. ,  FRIEDMAN, Paula, N. ,  GORDON, Julian ,  GRANADOS, Edward, N. ,  HODGES, Steven, C. ,  KLASS, Michael, R. ,  KRATOCHVIL, Jon, D. ,  RUSSELL, John, C. ,  STROUPE, Stephen, D.

IPC: C07K14/47 ,  C07K16/30 ,  C12N15/63 ,  C12N5/10 ,  G01N33/53 ,  G01N33/574 ,  G01N33/577

CPC classification number: C07K14/47 ,  C07K16/3015 ,  C07K2317/34 ,  C12Q1/6886 ,  C12Q2600/136

公开(公告)号：EP0749583B1

公开(公告)日：2002-08-28

申请号：EP95912800.0

申请日：1995-03-06

Applicant: Abbott Laboratories

Inventor： KIM, Young, Ran ,  STROUPE, Stephen, D.

IPC: G01N33/72

CPC classification number: G01N33/5094 ,  G01N33/721 ,  Y10T436/10 ,  Y10T436/107497 ,  Y10T436/25375

Abstract: A cyanide-free method and reagent for determining the concentration of total hemoglobin in a whole blood sample accurately in less than 10 seconds comprising a ligand selected from the group consisting of imidazole, imidazole derivatives, N-hydroxyacetamide, N-hydroxyl amine, pyridine, oxazole, thiazole, pyrazole, pyrimidine, purine, quinoline, and isoquinoline, and a surfactant with strong erythrolytic capability selected from the group consisting of lauryl dimethylamine oxide and octylphenoxy polyethoxyethanol. The reagent pH is adjusted to about 11 to about 14. Rapid mixing of the reagent with a blood sample leads to the formation of a stable chromogen whose absorbance can be measured between 540 and 550 nm. The cyanide-free reagent is ideal for use on an automated high through-put clinical hematology analyzer.

公开(公告)号：EP1144449A1

公开(公告)日：2001-10-17

申请号：EP00903373.9

申请日：2000-01-21

Applicant: ABBOTT LABORATORIES

Inventor： BILLING-MEDEL, Patricia, A. ,  COHEN, Maurice ,  COLPITTS, Tracey, L. ,  FRIEDMAN, Paula, N. ,  GORDON, Julian ,  GRANADOS, Edward, N. ,  HODGES, Steven, C. ,  KLASS, Michael, R. ,  KRATOCHVIL, Jon, D. ,  RUSSELL, John, C. ,  STROUPE, Stephen, D.

IPC: C07K14/47 ,  C07K16/30 ,  C12N15/63 ,  C12N5/10 ,  G01N33/53 ,  G01N33/574 ,  G01N33/577

CPC classification number: C07K14/47 ,  C07K16/3015 ,  C07K2317/34 ,  C12Q1/6886 ,  C12Q2600/136

Abstract: A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as BS322 and transcribed from breast tissue, is described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, in vivo imaging, preventing or treating, or determining the predisposition of an individual to diseases and conditions of the breast, such as breast cancer. Also provided are antibodies which specifically bind to BS322-encoded polypeptide or protein, and agonists or inhibitors which prevent action of the tissue-specific BS322 polypeptide, which molecules are useful for the therapeutic treatment of breast diseases, tumors or metastases.

公开(公告)号：EP1032689A1

公开(公告)日：2000-09-06

申请号：EP98958623.5

申请日：1998-11-17

Applicant: ABBOTT LABORATORIES

Inventor： BILLING-MEDEL, Patricia, A. ,  COHEN, Maurice ,  COLPITTS, Tracey, L. ,  FRIEDMAN, Paula, N. ,  GORDON, Julian ,  GRANADOS, Edward, N. ,  HODGES, Steven, C. ,  KLASS, Michael, R. ,  KRATOCHVIL, Jon, D. ,  ROBERTS-RAPP, Lisa ,  RUSSELL, John, C. ,  STROUPE, Stephen, D.

IPC: C12N15/63 ,  C12N5/10 ,  C07K14/47 ,  C07K16/30 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/574 ,  G01N33/577 ,  A61K38/17

CPC classification number: C07K14/47 ,  A61K38/00 ,  C12Q1/6886 ,  C12Q2600/136

Abstract: A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as BS274 and transcribed from breast tissue, is described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, in vivo imaging, preventing or treating, or determining the predisposition of an individual to diseases and conditions of the breast, such as breast cancer. Also provided are antibodies which specifically bind to BS274-encoded polypeptide or protein, and agonists or inhibitors which prevent action of the tissue-specific BS274 polypeptide, which molecules are useful for the therapeutic treatment of breast diseases, tumors or metastases.

公开(公告)号：EP1015625A2

公开(公告)日：2000-07-05

申请号：EP98948329.2

申请日：1998-09-18

Applicant: ABBOTT LABORATORIES

Inventor： BILLING-MEDEL, Patricia, A. ,  COHEN, Maurice ,  COLPITTS, Tracey, L. ,  FRIEDMAN, Paula, N. ,  GORDON, Julian ,  GRANADOS, Edward, N. ,  HODGES, Steven, C. ,  KLASS, Michael, R. ,  KRATOCHVIL, Jon, D. ,  MERCHANT, Barbara, T. ,  ROBERTS-RAPP, Lisa ,  RUSSELL, John, C. ,  STROUPE, Stephen, D.

IPC: C12Q1/00

CPC classification number: C07K16/3015 ,  A61K38/00 ,  A61K2039/51 ,  C07K14/705 ,  C07K16/3069 ,  C12Q1/6886 ,  C12Q2600/136

Abstract: A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as PS214 and transcribed from prostate, breast, or ovary tissue, is described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, in vivo imaging, preventing or treating, or determining the predisposition of an individual to diseases and conditions of the prostate, breast, or ovary, such as prostate, breast, or ovarian cancer. Also provided are antibodies which specifically bind to PS214-encoded polypeptide or protein, and agonists or inhibitors which prevent action of the tissue-specific PS214 polypeptide, which molecules are useful for the therapeutic treatment of prostate, breast, or ovary diseases, tumors or metastases.

公开(公告)号：EP1012343A1

公开(公告)日：2000-06-28

申请号：EP98948162.7

申请日：1998-09-11

Applicant: Abbott Laboratories

Inventor： BILLING-MEDEL, Patricia, A. ,  COHEN, Maurice ,  COLPITTS, Tracey, L. ,  FRIEDMAN, Paula, N. ,  GORDON, Julian ,  GRANADOS, Edward, N. ,  HODGES, Steven, C. ,  KLASS, Michael, R. ,  KRATOCHVIL, Jon, D. ,  RUSSELL, John, C. ,  STROUPE, Stephen, D.

IPC: C12Q1/68 ,  C12N15/12 ,  C12N5/10 ,  C12N9/02 ,  C07K16/40 ,  G01N33/574

CPC classification number: C07K16/18 ,  C07K14/47 ,  C12Q1/6886 ,  C12Q2600/136 ,  G01N33/57434

Abstract: Sequences useful for the detecting, diagnosing, staging, monitoring, prognosticating, in vivo imaging, preventing or treating, or determining the predisposition of an individual to diseases and conditions of the prostate, such as prostate cancer, are described. These sequences are designated as PS213 sequences herein. Also provided are antibodies which specifically bind to PS213-encoded polypeptide or protein, and agonists or inhibitors which prevent action of the tissue-specific PS213 polypeptide, which molecules are useful for the therapeutic treatment of prostate diseases, tumors or metastases.

公开(公告)号：EP0586590B1

公开(公告)日：1999-07-07

申请号：EP92913618.2

申请日：1992-04-10

Applicant: ABBOTT LABORATORIES

Inventor： ADAMCZYK, Janina ,  BERRY, Daniel, S. ,  FICO, Rosario ,  JOU, Yi-Her ,  STROUPE, Stephen, D.

IPC: C12Q1/00 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/536 ,  G01N33/537 ,  G01N33/538 ,  G01N33/541 ,  G01N33/543 ,  G01N33/544 ,  G01N33/546 ,  G01N33/532 ,  G01N33/545

CPC classification number: G01N33/54393 ,  G01N33/538 ,  G01N33/54353 ,  G01N33/544

Abstract: The present invention includes novel assays and reagents using a capture reagent, involving a specific binding member attached to a polymeric anionic substance, and a solid phase material containing a capture or reaction zone including a polymeric cationic substance. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. With an appropriate indicator reagent, sandwich, competitive and indirect assays can be performed. Preferably, the indicator reagent includes a nonspecific binding blocker to reduce the nonspecific binding between the indicator reagent and the solid phase, without interfering with the reaction between the capture reagent and the solid phase. Suitable nonspecific binding blockers include unbound or free polyanionic materials such as dextran sulfate, heparin, carboxymethyl dextran, carboxymethyl cellulose, pentosan polysulfate, inositol hexasulfate or beta -cyclodextrin sulfate.

公开(公告)号：EP0586574B1

公开(公告)日：1997-12-10

申请号：EP92913231.4

申请日：1992-04-10

Applicant: ABBOTT LABORATORIES

Inventor： KLINE, Steven ,  JOU, Yi-Her ,  STROUPE, Stephen, D.

IPC: G01N33/536 ,  G01N33/537 ,  G01N33/538 ,  G01N33/541 ,  G01N33/543 ,  G01N33/544 ,  G01N33/546 ,  G01N33/551 ,  G01N33/553 ,  G01N33/94

CPC classification number: G01N33/543 ,  G01N33/532 ,  G01N33/545 ,  G01N33/56988 ,  G01N33/57473 ,  G01N33/58 ,  G01N33/743 ,  G01N33/76 ,  G01N33/9453

Abstract: The present invention includes novel digoxin assays employing a capture reagent, involving a first binding member conjugated to a polymeric anion substance, and a solid phase material containing a reaction site comprising a polymeric cation substance having a nitrogen content of at least about two percent. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex.

公开(公告)号：EP0227795B2

公开(公告)日：2002-07-24

申请号：EP86904513.8

申请日：1986-06-13

Applicant: Amgen Inc., ,  ABBOTT LABORATORIES

Inventor： SNITMAN, David, L. ,  STROUPE, Stephen, D.

IPC: C12Q1/68 ,  G01N33/53 ,  C12N15/00

CPC classification number: G01N33/54353 ,  C12Q1/6804 ,  C12Q1/6813 ,  C12Q1/705 ,  G01N33/532

Abstract: A method and a kit for the isolation and quantitative detection of a selected target nucleic acid sequence from solution employing two probes. A first probe is complementary to one portion of the target and is covalently attached to a first complexing agent (e.g., either an antigen or an antibody). The second probe is complementary to a different portion of the target and is associated with a reporter group. Following hybridization of the target and two probes in solution, a solid support coated with a second complexing agent (i.e., a corresponding antibody or antigen) capable of binding to the first complexing agent on the first probe is employed to immobilize the target-probe hybrid complex. A plurality of types of first probes may be used. Each type is attached to the same sort of complexing agent but each includes a nucleic acid sequence which is complementary to a different portion of the target.