Abstract:
A bubble flushing aspirating and dispensing syringe having precision and volumetric accuracy, particularly for use in an automated, continuous and random access analytical system, is disclosed. The syringe comprises a piston (124) within a bore (128), the bore having seal means at a first end and a closed end (130) at a second end, wherein the piston forms an annulus (138) with the bore wall and seal means, and is capable of reciprocating therein. Fluid inlet (134) and fluid outlet (136) means are in communication with the annulus (138) and bore, the fluid inlet and fluid outlet means being located between the seal means and the bore closed end. A fluid source is in communication with the inlet means and a fluid conduit is in communication with the outlet means and an open-ended release tip. A drive means is connected to the piston for reciprocating the piston within the bore. As a result, fluid from the inlet, when connected to a fluid supply, flows through the annulus (138) around the sides of the piston and out through the fluid outlet to the open-ended tip, thereby creating a cross-flow pattern in the annulus (138) around the piston as it reciprocates in the bore to flush bubbles through the outlet.
Abstract:
A bubble flushing aspirating and dispensing syringe having precision and volumetric accuracy, particularly for use in an automated, continuous and random access analytical system, is disclosed. The syringe comprises a piston (124) within a bore (128), the bore having seal means at a first end and a closed end (130) at a second end, wherein the piston forms an annulus (138) with the bore wall and seal means, and is capable of reciprocating therein. Fluid inlet (134) and fluid outlet (136) means are in communication with the annulus (138) and bore, the fluid inlet and fluid outlet means being located between the seal means and the bore closed end. A fluid source is in communication with the inlet means and a fluid conduit is in communication with the outlet means and an open-ended release tip. A drive means is connected to the piston for reciprocating the piston within the bore. As a result, fluid from the inlet, when connected to a fluid supply, flows through the annulus (138) around the sides of the piston and out through the fluid outlet to the open-ended tip, thereby creating a cross-flow pattern in the annulus (138) around the piston as it reciprocates in the bore to flush bubbles through the outlet.
Abstract:
The present invention relates to a method for simultaneously performing at least two assays, said assays comprising a plurality of activities, for a plurality of liquid samples on a continuous analytical system, said method comprising the steps of: combining an aliquot of a first liquid sample with at least one reagent in a first reaction container to form a first assay reaction mixture for said first liquid sample; combining an aliquot of a second liquid sample with at least one reagent in a second reaction container to form a second assay reaction mixture for said second liquid sample; incubating said first and said second assay reaction mixtures at least one time; performing activities associated with each assay other than said combining and said incubating on the first and second assay reaction mixtures to complete each assay, said other activities including analyzing the incubated assay reaction mixtures; and scheduling the steps of said combining, said incubating, and said performing activities other than said combining and said incubating associated with each of the assays according to a predetermined protocol, said protocol specifying: (a) what activities are to be performed for a given assay; (b) an order in which said activities of (a) are to be performed; (c) at least one incubation period between said activities of (a), said at least one incubation period comprising a nominal period of time for the performance of an incubating step between activities of (a) and a specified window of time for varying the duration of the nominal period between activities of (a) to optimize performance; (d) how said activities of (a) are to be performed; and (e) duration of said activities of (a).
Abstract:
A liquid level sensing device for sensing the level of a liquid in a container and an automated, continuous and random access analytical system containing such liquid level sensing device and capable of simultaneously effecting multiple assays of a plurality of liquid samples are disclosed. The liquid level sensing device comprises: (a) means for producing an electrical sinusoidal signal; (b) means for evaluating the amount of signal as it propagates from a probe to a sense antenna; (c) circuit means for creating an electrical signal wherein said electrical signal changes when a probe contacts liquid; (d) signal processing means for enhancing the electrical signal and for degrading and suppressing signals not associated with the probe contacting said liquid; (e) decision circuit means for causing a basic digital output indicating either the presence or absence of liquid; and (f) means for utilizing the digital signal for controlling motion, and wherein said sensing device detects signal change and rate of signal change.
Abstract:
The invention relates to an automated liquid level sensing system for detecting the presence of liquid in a container, said liquid level sensing system comprising: a vertically oriented, electrically conductive probe positioned above said container; means for vertically moving said probe into and out of said container; a signal source electrically connected to said probe, said signal source energizing said probe with an electrical signal and causing said probe to transmit said electrical signal; a receiving antenna positioned below said container for receiving said transmitted electrical signal; means for analyzing said received electrical signal for indications that said probe has contacted liquid in said container; means for transferring said received electrical signal from said receiving antenna to said analyzing means; and means for indicating that liquid has been detected. Further, the invention also provides for a method for automatically detecting the presence of liquid in a container, said method comprising the steps of: vertically positioning an electrically conductive probe above said container; vertically moving said probe into and out of said container; electrically connecting a signal source to said probe, said signal source energizing said probe with an electrical signal and causing said probe to transmit said electrical signal; positioning a receiving antenna below said container for receiving said transmitted electrical signal; analyzing said received electrical signal for indications that said probe has contacted liquid in said container; transferring said received electrical signal from said receiving antenna to said analyzing means; and indicating that liquid has been detected.
Abstract:
The structure embodiments disclosed relate to determination of an item of interest in a sample. One embodiment relates to a structure which comprises a process path (10). The process path (10) comprises a process lane (28) including a process step performance lane (62) where a process step is performed, and a process step avoidance lane (64) where the process step is avoided. A first prime mover (24) is operatively connected with the process path (10) for moving a container (15) holding the sample along the process path (10). A first pipetting system (198) is operatively associated with the process path (10) for introducing the sample to the container (15). A second pipetting system (132, 134) is operatively associated with the process path (10) for introducing a reagent to the container (15). A device (86) is operatively connected with the process path (10) and is selectively engagable with the container (15) for mixing the sample and the reagent in the container (15). A second prime mover (44) is operatively connected with the process path (10) for selectively positioning the container (15) in a selected one of the process step performance lane (62) and the process step avoidance lane (64). A reader (138) is operatively connected with the process path (10) for determining the item of interest in the sample based upon a reaction between the sample and the reagent. The method embodiments disclosed relate to determination of an item of interest in a sample. In one method, a process path (10) comprising a process lane (28) including a process step performance lane (62) where a process step is performed, and a process step avoidance lane (64) where the process step is avoided is provided. A container (15) holding the sample is moved along the process path (10). The sample is introduced to the container (15). A reagent is introduced to the container (15). The sample and the reagent are mixed in the container (15). The container (15) is selectively positioned in a selected one of the process step performance lane (62) and the process step avoidance lane (64). The item of interest in the sample is determined based upon a reaction between the sample and the reagent.