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    MICROORGANISMS AND METHODS FOR PRODUCING 2,4-PENTADIENOATE, BUTADIENE, PROPYLENE, 1,3-BUTANEDIOL AND RELATED ALCOHOLS
    32.
    发明申请
    MICROORGANISMS AND METHODS FOR PRODUCING 2,4-PENTADIENOATE, BUTADIENE, PROPYLENE, 1,3-BUTANEDIOL AND RELATED ALCOHOLS 审中-公开
    用于生产2,4-戊二烯酸酯，丁二烯，丙烯，1,3-丁二醇和相关醇的微生物和方法

    公开(公告)号：WO2013028519A1

    公开(公告)日：2013-02-28

    申请号：PCT/US2012/051347

    申请日：2012-08-17

    Applicant: GENOMATICA, INC. ,  OSTERHOUT, Robin, E. ,  BURGARD, Anthony, P. ,  BURK, Mark, J.

    Inventor： OSTERHOUT, Robin, E. ,  BURGARD, Anthony, P. ,  BURK, Mark, J.

    IPC: C12P7/18

    CPC classification number: C12P7/16 ,  C12N15/70 ,  C12P5/026 ,  C12P7/04 ,  C12P7/18 ,  C12P7/40 ,  Y02E50/10

    Abstract: The invention provides non-naturally occurring microbial organisms containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathways comprising at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. The invention additionally provides methods of using such microbial organisms to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol, by culturing a non- naturally occurring microbial organism containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol pathways as described herein under conditions and for a sufficient period of time to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol.

    Abstract translation: 本发明提供包含2,4-戊二烯酸，丁二烯，丙烯，1,3-丁二醇，巴豆醇或3-丁烯-1-醇途径的非天然存在的微生物生物体，其包含至少一种外源性 编码丁二烯途径酶的核酸，其表达量足以产生2,4-戊二烯酸，丁二烯，丙烯，1,3-丁二醇，巴豆醇或3-丁烯-1-醇。 本发明另外提供了通过培养含有2个非天然存在的微生物的微生物来生产2,4-戊二烯酸，丁二烯，丙烯，1,3-丁二醇，巴豆醇或3-丁烯-1-醇的方法 ，4-戊二烯酸酯，丁二烯，丙烯，1,3-丁二醇，巴豆醇或3-丁烯-1-醇途径在一定条件下和足够的时间内生成2,4-戊二烯酸，丁二烯，丙烯， 1,3-丁二醇，巴豆醇或3-丁烯-1-醇。

    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF 1.4- CYCLOHEXANEDIMETHANOL
    34.
    发明申请
    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF 1.4- CYCLOHEXANEDIMETHANOL 审中-公开
    用于生产1,4-环己烷二甲醇的微生物和方法

    公开(公告)号：WO2012082978A1

    公开(公告)日：2012-06-21

    申请号：PCT/US2011/065048

    申请日：2011-12-15

    Applicant: GENOMATICA, INC. ,  PHARKYA, Priti ,  BURK, Mark, J.

    Inventor： PHARKYA, Priti ,  BURK, Mark, J.

    IPC: C12P7/18

    CPC classification number: C12P7/02 ,  C12N15/52 ,  C12N15/70 ,  C12N15/74 ,  C12N15/75 ,  C12N15/76 ,  C12N15/78 ,  C12N15/81 ,  C12N15/815 ,  C12P7/18

    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,4-cyclohexanedimethanol pathway that includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4- cyclohexanedimethanol. A method for producing 1,4-cyclohexanedimethanol includes culturing a non-naturally occurring microbial organism having a 1,4-cyclohexanedimethanol pathway. The pathway includes at least one exogenous nucleic acid encoding a 1,4- cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4- cyclohexanedimethanol, under conditions and for a sufficient period of time to produce 1,4- cyclohexanedimethanol.

    Abstract translation: 非天然存在的微生物体包括具有1,4-环己烷二甲醇途径的微生物生物体，其包含至少一种编码1,4-环己烷二甲醇途径酶的外源核酸，其以足够的量表达以产生1,4-环己烷二甲醇。 生产1,4-环己烷二甲醇的方法包括培养具有1,4-环己烷二甲醇途径的非天然存在的微生物。 所述途径包括至少一种编码1,4-环己烷二甲醇途径酶的外源核酸，其在足以在足够的时间内产生1,4-环己烷二甲醇以足够量产生1,4-环己烷二甲醇。

    PROCESS OF SEPARATING COMPONENTS OF A FERMENTATION BROTH
    36.
    发明申请
    PROCESS OF SEPARATING COMPONENTS OF A FERMENTATION BROTH 审中-公开
    分离发酵组分的方法

    公开(公告)号：WO2010141780A1

    公开(公告)日：2010-12-09

    申请号：PCT/US2010/037329

    申请日：2010-06-03

    Applicant: GENOMATICA, INC. ,  CLARK, Warren ,  JAPS, Michael ,  BURK, Mark, J.

    Inventor： CLARK, Warren ,  JAPS, Michael ,  BURK, Mark, J.

    IPC: C07C31/18 ,  C07C33/02

    CPC classification number: C07C29/76 ,  C12P7/18 ,  C07C31/207

    Abstract: A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4- BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO.

    Abstract translation: 从发酵液中分离1,4-丁二醇（1,4-BDO）的方法包括从含有细胞的固体部分中分离富含1,4-BDO的液体馏分，从所述液体馏分中除去水，从所述的 液体馏分，并纯化1,4-BDO。 生产1,4-BDO的方法包括在发酵罐中培养产生1,4-BDO的微生物足够长的时间以产生1,4-BDO。 1,4-生产BDO的微生物包括具有1,4-BDO途径的微生物，其具有编码1,4-BDO途径酶的一种或多种外源基因和/或一种或多种基因中断。 生产1,4-BDO的方法还包括分离1,4-BDO。

    COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS
    38.
    发明申请
    COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS 审中-公开
    1,4-丁二醇及其前体的生物合成的组合物和方法

    公开(公告)号：WO2008115840A2

    公开(公告)日：2008-09-25

    申请号：PCT/US2008/057168

    申请日：2008-03-14

    Applicant: GENOMATICA, INC. ,  BURK, Mark, J. ,  VAN DIEN, Stephen, J. ,  BURGARD, Anthony ,  NIU, Wei

    Inventor： BURK, Mark, J. ,  VAN DIEN, Stephen, J. ,  BURGARD, Anthony ,  NIU, Wei

    IPC: C12N1/21

    CPC classification number: C12N15/52 ,  B01D3/002 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12N9/93 ,  C12N15/70 ,  C12N15/81 ,  C12P7/18 ,  C12P7/42 ,  C12P7/52 ,  C12P17/04 ,  C12Y101/01061 ,  C12Y102/01076 ,  C12Y401/01071 ,  C12Y602/01004 ,  Y02P20/52

    Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4- butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a A- hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or α-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric A- hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium.

    Abstract translation: 本发明提供一种非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）生物合成途径的微生物生物，其具有至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛 脱氢酶或α-酮戊二酸脱羧酶，其中外源核酸以足够的量表达以产生单体4-羟基丁酸（4-HB）。 还提供了非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）和1,4-丁二醇（BDO）生物合成途径的微生物生物，该途径包括至少一种编码4-羟基丁酸脱氢酶的外源核酸 ，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛脱氢酶，4-羟基丁酸酯：CoA转移酶，4-丁酸激酶，磷酸转移酶，α-酮戊二酸脱羧酶，醛脱氢酶，醇脱氢酶或醛/醇脱氢酶，其中外源核酸为 表达量足以生产1,4-丁二醇（BDO）。 另外提供了用于生产4-HB的方法。 所述方法包括培养具有4-羟基丁酸（4-HB）生物合成途径的非天然存在的微生物生物，其包含至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，辅酶A依赖性琥珀酸半醛脱氢酶或 - 酮戊二酸脱羧酶在充分的厌氧条件下持续足够的时间以产生单体的4-羟基丁酸（4-HB）。 进一步提供一种生产BDO的方法。 该方法包括培养非天然存在的微生物生物催化剂，其包含具有4-羟基丁酸（4-HB）和1,4-丁二醇（BDO）生物合成途径的微生物生物，所述途径包括至少一种编码4- 羟基丁酸脱氢酶，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸脱氢酶，4-羟基丁酸酯：CoA转移酶，4-羟基丁酸酯激酶，磷酸转移羟基丁酸酶，α-酮戊二酸脱羧酶，醛脱氢酶，醇脱氢酶或醛/醇脱氢酶， 时间来生产1,4-丁二醇（BDO）。 4-HB和/或BDO产物可以分泌到培养基中。

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