公开(公告)号：JP2004283776A

公开(公告)日：2004-10-14

申请号：JP2003081498

申请日：2003-03-24

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  SUGIYAMA NORIKAZU ,  FUKUSHIMA MASANORI ,  MORI KANEO

IPC: C02F1/30 ,  C02F1/48

Abstract: PROBLEM TO BE SOLVED: To provide a production apparatus for functional water which has cell activation effects, increases immune functions, cell proliferation capacity, and ATP (adenosine triphosphate) content in cells, and brings beneficial effects on living bodies such as human body, and a production method therefor.
SOLUTION: The production apparatus for the functional water has a magnetic shielding means for forming a magnetic shielding space by shielding a magnetic field from the outside of a predetermined space, a water storage container for holding water to be treated inside the magnetic shielding space, a direct current magnetic field application means and a low-frequency micromagnetic field application means for obtaining the functional water by simultaneously applying steady direct current magnetic field and low-frequency micromagnetic field to the water to be treated inside the water storage container, and an angle adjustment means for holding the direct current magnetic field application means and the low-frequency micromagnetic field application means so that the angle between a first axis corresponding to the direction of the direct current magnetic field and a second axis corresponding to the direction of the low-frequency micromagnetic field becomes a predetermined angle.
COPYRIGHT: (C)2005,JPO&NCIPI

Abstract translation: 要解决的问题：为了提供具有细胞活化作用的功能性水的生产装置，增加细胞中的免疫功能，细胞增殖能力和ATP（三磷酸腺苷）含量，并且对人体如人体产生有益效果 身体及其生产方法。 解决方案：功能水的制造装置具有磁屏蔽装置，用于通过屏蔽来自预定空间的外部的磁场形成磁屏蔽空间，用于在磁屏蔽内保持被处理水的储水容器 空间，直流磁场施加装置和低频微磁场施加装置，用于通过向储水容器内的待处理水施加稳定的直流磁场和低频微磁场来获得功能水;以及 用于保持直流磁场施加装置和低频微磁场施加装置的角度调节装置，使得对应于直流磁场方向的第一轴线和对应于直流磁场施加装置的方向的第二轴线之间的角度 低频微磁场变为预定角度。 版权所有（C）2005，JPO＆NCIPI

公开(公告)号：JPH11271227A

公开(公告)日：1999-10-05

申请号：JP7042798

申请日：1998-03-19

Applicant: HAMAMATSU PHOTONICS KK

Inventor： MIZUGUCHI YOSHINORI ,  KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G01N21/64 ,  G01N21/76 ,  G01N21/91 ,  G01N33/483

Abstract: PROBLEM TO BE SOLVED: To provide a fluorescent measurement device for sufficiently detecting fluorecent lights from samples independently of the typed of the samples. SOLUTION: A fluorescent measurement device 1 for radiating an exciting light from the bottom 4a side of a microplate 4 to samples S in a plurality of wells 3 on the microplate 4 to detect the fluorescent light from the samples S at the bottom 4a side includes an exciting light generator 5 for generating the exciting light, a plurality of optical fibers 13 for distributing the exciting light from the generator 5 corresponding to the plurality of wells 3, optical fibers 15 for transferring the fluorescent lights from the samples S in the plurality of wells 3, a fluorescent light detector 18 for detecting the fluorescent lights transferred by the optical fibers 15, and fiber moving mechanisms provided on the bottom 4a side of the microplate 4 corresponding to the plurality of wells 3 for making a plurality of optical fiber bundle portions 17 with the emission ends 13b of the optical fibers 13 and the injection ends 15a of the optical fibers 15 combined approach to or isolate from the bottom 4a of the microplate 4. In this case, the fluorescent lights of proper intensity corresponding to the nature of cells are detected.

公开(公告)号：JP2013108994A

公开(公告)日：2013-06-06

申请号：JP2013043341

申请日：2013-03-05

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  ITO HIRAYOSHI

IPC: G01N21/64

Abstract: PROBLEM TO BE SOLVED: To provide a light measuring apparatus capable of reducing background light noise from a microplate.SOLUTION: A light irradiating device for irradiating a microplate 20 on which a plurality of wells 21 for housing a measurement object A are provided, with irradiation light, and a light measuring apparatus comprising the light irradiating device comprise: a light guiding member 61 including a main surface 61a on which a plurality of recesses 61e are formed, and a side surface 61b substantially orthogonal to the main surface 61a; and a light source 62 for making the irradiation light incident on the light guiding member 61 from the side surface 61b of the light guiding member 61. The recesses 61e include an opening on the main surface 61a. The light guiding member 61 can be disposed so that the bottom surface of the wells 21 and the opening of the recesses 61e face each other. The irradiation light made incident on the light guiding member from the side surface 61b is refracted and reflected on the side surface of the recesses 61e, and then emitted from the opening of the recesses 61e to be made incident on the bottom surface of the wells 21 of the microplate 20.

Abstract translation: 要解决的问题：提供能够降低微板的背景光噪声的光测量装置。 解决方案：照射照射光的照射装置，用于照射其上设置有用于容纳测量对象A的多个孔21的微板20，以及包括该光照射装置的光测量装置包括：导光构件 61包括形成有多个凹部61e的主表面61a和与主表面61a大致正交的侧表面61b; 以及用于使照射光从导光构件61的侧面61b入射到导光构件61上的光源62.凹部61e包括在主表面61a上的开口。 导光构件61可以设置为使得孔21的底表面和凹部61e的开口彼此面对。 从侧面61b入射在导光构件上的照射光在凹部61e的侧面折射反射，然后从凹部61e的开口部射出，入射到孔21的底面 的微孔板20.版权所有（C）2013，JPO＆INPIT

公开(公告)号：JP2013021961A

公开(公告)日：2013-02-04

申请号：JP2011159028

申请日：2011-07-20

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  SUGIYAMA NORIKAZU

IPC: C12M1/34 ,  C12N5/071 ,  G01N21/17 ,  G01N33/483

Abstract: PROBLEM TO BE SOLVED: To evaluate pumping power of myocardial cell in high accuracy.SOLUTION: There is provided a cell measurement method is a cell evaluation method to evaluate the pumping power of a myocardial cell C, and includes an image acquiring step S1 to acquire an optical image data 60 of the myocardial cell C attached on a transparent specimen case, a region setting step S2 to set an analyzing region RC indicating a region occupied by the myocardial cell C based on luminance values of pixels of the optical image data 60, a data acquisition step S3 to acquire at least one of thickness data showing the temporal change of the thickness of the myocardial cell C and areal data showing the temporal change of the area of the region occupied by the myocardial cell C based on the pixels of the analyzing region RC, and an evaluation step S6 to evaluate the pumping power of the myocardial cell C based on at least one of the areal data and the thickness data acquired by the data acquisition step S3.

Abstract translation: 要解决的问题：以高精度评估心肌细胞的泵浦功率。 解决方案：提供了一种细胞测量方法，其是评估心肌细胞C的泵浦功率的细胞评估方法，并且包括图像获取步骤S1，以获取附着在心肌细胞C上的心肌细胞C的光学图像数据60 透明样品盒，区域设定步骤S2，根据光学图像数据60的像素的亮度值设定指示心肌细胞C所占据的区域的分析区域RC，获取厚度数据 显示了基于分析区域RC的像素的心肌细胞C的厚度的时间变化和显示心肌细胞C占据的区域的面积的时间变化的面积数据，以及评估步骤S6，以评估泵送 基于通过数据获取步骤S3获取的面积数据和厚度数据中的至少一个，心肌细胞C的功率。 版权所有（C）2013，JPO＆INPIT

公开(公告)号：JP2010230397A

公开(公告)日：2010-10-14

申请号：JP2009076601

申请日：2009-03-26

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  ITO HIRAYOSHI

IPC: G01N21/64 ,  G01N21/01

Abstract: PROBLEM TO BE SOLVED: To provide a light irradiation device capable of reducing background light noise from a microplate, and a light measuring instrument.
SOLUTION: In the light irradiation device for irradiating the microplate 20, to which a plurality of wells for housing a measuring target are provided, with irradiation light and the light measuring instrument equipped with the light irradiation device, a light guide member 61, which includes a main surface 61a having a plurality of protrusions 61e almost same in a shape formed thereto, the back 61h being the surface on the side opposite to the main surface 61a and the side surface 61b almost crossing the main surface 61a at an almost right angle, and a light source device 62 applying irradiation light 61b to the light guide member 61 from the side surface 61b of the light guide member 61 are provided. The protrusions 61e have upper surfaces 61f almost parallel to the main surface 61a and the light guide member 61 can be arranged so that the upper surfaces 61f of the protrusions 61e come into contact with the back 23 of the microplate 20. Further, the light guide member 61 can be arranged so that the upper surfaces 61f of the protrusions 61e may be opposed to the bases of the wells 21 of the microplate 20.
COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 要解决的问题：提供能够降低来自微孔板的背景光噪声的光照射装置和光测量仪器。 解决方案：在照射照射光的照射装置中，照射照射光的多个用于容纳测量对象的孔的微板20和配备有该光照射装置的光测量仪器的光照射装置， ，其包括主表面61a，其具有与其形成的形状几乎相同的多个突起61e，后面61h是与主表面61a相反的一侧的表面和几乎与主表面61a几乎相交的侧表面61b 设置有从导光构件61的侧面61b向照射光61b施加照射光61b的光源装置62。 突起61e具有几乎平行于主表面61a的上表面61f，并且导光构件61可以布置成使得突起61e的上表面61f与微板20的背面23接触。此外，光导 构件61可以布置成使得突起61e的上表面61f可以与微板20的孔21的基部相对。版权所有（C）2011，JPO和INPIT

公开(公告)号：JP2010230396A

公开(公告)日：2010-10-14

申请号：JP2009076595

申请日：2009-03-26

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  ITO HIRAYOSHI

IPC: G01N21/64 ,  G01N21/01 ,  G01N21/03

Abstract: PROBLEM TO BE SOLVED: To provide a light irradiation device capable of reducing the background light noise from a microplate, and a light measuring instrument.
SOLUTION: In the light irradiation device for irradiating the microplate 20, to which a plurality of wells 21 for housing a measuring target A are provided, with irradiation light and the light measuring instrument equipped with the light irradiation device, a light guide member 61, which has a main surface 61a having a plurality of recesses 61e formed thereto and the side surface 61b crossing the main surface 61a at an almost right angle and a light source 62 for applying the irradiation light to the light guide member 61 from the side surface 61b of the light guide member 61 are provided. The recesses 61e have openings in the main surface 61a and the light guide member 61 can be arranged so that the bases of the wells 21 and the openings of the recesses 61e are opposed to each other. The irradiation light incident on the light guide member from the side surface 61b is refracted and reflected by the side surfaces of the recesses 61e and emitted from the openings of the recesses 61e to be incident on the bases of the wells 21 of the microplate 20.
COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 要解决的问题：提供能够降低来自微板的背景光噪声的光照射装置和光测量装置。 解决方案：在照射照射光的照射装置中，照射照射光的多个用于容纳测定对象物A的孔21的微板20和配备有该照射装置的光测量装置， 构件61具有主表面61a，其具有形成于其上的多个凹部61e和与主表面61a成几乎直角的侧面61b和用于将照射光从该导光构件61施加的光源62 设置导光部件61的侧面61b。 凹部61e在主表面61a中具有开口，并且导光构件61可以布置成使得孔21的基部和凹部61e的开口彼此相对。 从侧面61b入射在导光构件上的照射光被凹部61e的侧面折射并反射，从凹部61e的开口射出，入射到微孔板20的孔21的基部。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2006126073A

公开(公告)日：2006-05-18

申请号：JP2004316512

申请日：2004-10-29

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI ,  HISADA SHIROSHI

IPC: G01N33/48 ,  C12Q1/02 ,  G01N33/15 ,  G01N33/483 ,  G01N33/50

Abstract: PROBLEM TO BE SOLVED: To provide a highly sensitive and simple method of measuring membrane potential change utilizing a membrane potential sensitive fluorescent pigment.
SOLUTION: The method of measuring the membrane potential change has a step for acquiring a pigment-introduced cell by executing introduction of a fluorescence quenching pigment into an intracellular or extracellular medium and staining of a cell membrane of a cell by the membrane potential sensitive fluorescent pigment, simultaneously or in the order wherein either is executed in advance; and a step for measuring with the passage of time the fluorescence intensity generated from the membrane potential sensitive fluorescent pigment by irradiating the pigment-introduced cell with excitation light.
COPYRIGHT: (C)2006,JPO&NCIPI

Abstract translation: 要解决的问题：提供利用膜电位敏感荧光颜料测量膜电位变化的高灵敏度和简单的方法。 解决方案：测量膜电位变化的方法具有通过将荧光猝灭色素引入细胞内或细胞外介质并通过膜电位染色细胞的细胞膜来获得引入颜料的细胞的步骤 同时或以预先执行的顺序进行敏感的荧光颜料; 以及通过用激发光照射引入颜料的细胞，随着时间的推移测量从膜电位敏感荧光颜料产生的荧光强度的步骤。 版权所有（C）2006，JPO＆NCIPI