公开(公告)号：EP2725346A4

公开(公告)日：2015-04-08

申请号：EP12802697

申请日：2012-06-19

Applicant: HAMAMATSU PHOTONICS KK

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G06T7/00 ,  G01N21/64

CPC classification number: G02B21/365 ,  G01N21/6452 ,  G01N21/6458 ,  G06K9/0014 ,  G06T7/0016 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30072

公开(公告)号：EP2725345A4

公开(公告)日：2014-12-17

申请号：EP12802485

申请日：2012-06-19

Applicant: HAMAMATSU PHOTONICS KK

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G01N21/64 ,  A61B10/00 ,  G01N33/48 ,  G06T7/00

CPC classification number: G02B21/365 ,  G01N21/6452 ,  G01N21/6458 ,  G06T7/0016 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30242

公开(公告)号：JP2007278985A

公开(公告)日：2007-10-25

申请号：JP2006108855

申请日：2006-04-11

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G01N21/64

Abstract: PROBLEM TO BE SOLVED: To provide a light measuring device, light measuring method, and to provide a light measuring program capable of improving the measuring sensitivity with respect to light from a sample. SOLUTION: A fluorescence measuring apparatus 1 for measuring light from the sample held in a well of a microplate 20 comprises an image acquiring section 40 for acquiring a two-dimensional photodetection image, and a data analysis device 50 for analyzing measuring data, including the photodetection image. The data analysis device 50 extracts an analysis region to be analyzed from the measurement region set, in response to the well on the photodetection image, by comparing two-dimensional light intensity distribution in the measurement region with a predetermined light intensity threshold, and performs analysis processings of the sample by using the data of the light intensity inside the analysis region as the analysis data. COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供一种光测量装置，光测量方法，并且提供能够提高相对于来自样品的光的测量灵敏度的光测量程序。 解决方案：用于测量保存在微板20的孔中的样品的光的荧光测量装置1包括用于获取二维光电图像的图像获取部分40和用于分析测量数据的数据分析装置50， 包括光电检测图像。 数据分析装置50通过将测量区域中的二维光强度分布与预定的光强度阈值进行比较，通过对光检测图像上的阱进行比较，从测量区域集中提取要分析的分析区域，并进行分析 通过使用分析区域内的光强度的数据作为分析数据来处理样品。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JPH10170444A

公开(公告)日：1998-06-26

申请号：JP32704996

申请日：1996-12-06

Applicant: HAMAMATSU PHOTONICS KK

Inventor： MIZUGUCHI YOSHINORI ,  MATSUBARA MASANORI

IPC: G01N21/64 ,  G01N21/17 ,  G01N21/78 ,  G01N35/02

Abstract: PROBLEM TO BE SOLVED: To quickly measure a fluorescence emitted from a sample or a light passing through the sample, by setting a guide member which guides a reagent to each well of a microplate and transmits the light. SOLUTION: A micropolate 4 is moved from an initial position to a position of a guide member 50 along a guide rail 2. A reagent is injected to each well of the microplate 4 through an opening part of the guide member 50 by a reagent dispenser 51. The microplate 4 is further moved along the guide rail 2 and arranged at a position of a guide member 19. When a leading end part 19a of the guide member 19 is illuminated by an exciting light, the exciting light passes through an opening part of the guide member 19 and is projected all at once to samples in the wells of the microplate 4. The light generated from each sample is input to a video camera 6, and a video signal is sent to an operation-processing unit 40 through an image-processing unit 35. An intensity of fluorescence or the like is thus calculated and measured in a short time.

公开(公告)号：JP2013003091A

公开(公告)日：2013-01-07

申请号：JP2011137314

申请日：2011-06-21

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G01N21/64 ,  G01N33/48 ,  G01N33/483

CPC classification number: G02B21/365 ,  G01N21/6452 ,  G01N21/6458 ,  G06T7/0016 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30242

Abstract: PROBLEM TO BE SOLVED: To specify a pixel whose luminance value chronologically changes, in an image containing light emitted from a sample.SOLUTION: An optical measurement device 1 is used for measuring light from a sample and includes: a motion image obtainment part 40 for obtaining motion image data of a two-dimensional light image containing light from a sample; and an analysis process part 51 for analyzing the motion image data. The analysis process part 51 includes: a luminance value data obtainment part 52a for obtaining luminance value data indicating chronological changes of a luminance value in plural pixels constituting a zone corresponding to plural wells; a luminance value extraction part 52b for extracting a peak value of a luminance value and a bottom value thereof from the luminance value data; and a pixel specifying part 52c for calculating an evaluation value evaluating a state of change in the luminance value on the basis of the peak and bottom values, and specifying a target pixel, being a target of analysis, from among the plural pixels on the basis of repetition of the evaluation value.

Abstract translation: 要解决的问题：在包含从样品发射的光的图像中指定其亮度值按时间顺序改变的像素。 解决方案：光学测量装置1用于测量来自样品的光，并包括：运动图像获取部分40，用于获得包含来自样本的光的二维光图像的运动图像数据; 以及用于分析运动图像数据的分析处理部分51。 分析处理部分51包括：亮度值数据获取部分52a，用于获得指示构成对应于多个井的区域的多个像素的亮度值的时间变化的亮度值数据; 亮度值提取部52b，用于从亮度值数据中提取亮度值的峰值及其底值; 以及像素指定部52c，用于基于峰值和下降值来计算评价亮度变化状况的评价值，并且从基准的多个像素中指定作为分析对象的目标像素 重复评估值。 版权所有（C）2013，JPO＆INPIT

公开(公告)号：JP2007298379A

公开(公告)日：2007-11-15

申请号：JP2006126346

申请日：2006-04-28

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G01N21/76 ,  G01N21/78

Abstract: PROBLEM TO BE SOLVED: To provide a device for measuring light emission capable of efficiently performing the measurement of multicolor emission, and to provide a method of measuring the light emission.
SOLUTION: The device 1 for measuring the emission from the sample held in each of the wells of a microplate 20 includes an image acquiring part 40 for acquiring a two-dimensional emission image, a light guide optical system 41 for guiding the two-dimensional image of the microplate 20 containing the emission from the sample to the image acquirig part 40 and the sheetlike filter member 30 arranged on a light path of the two-dimensional image. In the filter member 30, a plurality of optical filter parts are arranged in a two-dimensional array state so as to correspond to a plurality of the wells of the microplate 20 and constituted of a plurality of filter regions mutually different in light transmission characteristics with respect to a wavelength.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供一种用于测量能够有效执行多色发射测量的发光的装置，并提供一种测量发光的方法。 解决方案：用于测量保存在微板20的每个孔中的样品的发射的装置1包括用于获取二维发射图像的图像获取部40，用于引导二维发射图像的导光光学系统41 包含从样品发射到图像取出部分40的微板20的三维图像和布置在二维图像的光路上的片状过滤元件30。 在过滤构件30中，多个滤光器部件被布置成二维阵列状态，以对应于微板20的多个孔，并且由光传输特性互不相同的多个滤光器区域构成， 尊重波长。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JPH11271227A

公开(公告)日：1999-10-05

申请号：JP7042798

申请日：1998-03-19

Applicant: HAMAMATSU PHOTONICS KK

Inventor： MIZUGUCHI YOSHINORI ,  KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G01N21/64 ,  G01N21/76 ,  G01N21/91 ,  G01N33/483

Abstract: PROBLEM TO BE SOLVED: To provide a fluorescent measurement device for sufficiently detecting fluorecent lights from samples independently of the typed of the samples. SOLUTION: A fluorescent measurement device 1 for radiating an exciting light from the bottom 4a side of a microplate 4 to samples S in a plurality of wells 3 on the microplate 4 to detect the fluorescent light from the samples S at the bottom 4a side includes an exciting light generator 5 for generating the exciting light, a plurality of optical fibers 13 for distributing the exciting light from the generator 5 corresponding to the plurality of wells 3, optical fibers 15 for transferring the fluorescent lights from the samples S in the plurality of wells 3, a fluorescent light detector 18 for detecting the fluorescent lights transferred by the optical fibers 15, and fiber moving mechanisms provided on the bottom 4a side of the microplate 4 corresponding to the plurality of wells 3 for making a plurality of optical fiber bundle portions 17 with the emission ends 13b of the optical fibers 13 and the injection ends 15a of the optical fibers 15 combined approach to or isolate from the bottom 4a of the microplate 4. In this case, the fluorescent lights of proper intensity corresponding to the nature of cells are detected.

公开(公告)号：JP2007298380A

公开(公告)日：2007-11-15

申请号：JP2006126349

申请日：2006-04-28

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G01N21/03 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To provide a sample holding member which enables the efficient measurement of emission under various conditions such as the measurement of multicolor emission or the like.
SOLUTION: The sample holding member 2 is constituted of a microplate 20 having a plurality of wells 21, which respectively hold a sample, arranged thereto in a two-dimensional array state and the filter member 30 provided so as to be fixed at a predetermined position where the emission from the sample held in each of the wells 21 of the microplate 20 transmits. Further, the filter member 30 has a plurality of optical filter parts 31 arranged in a two-dimensional array state so as to correspond to a plurality of the wells 21 of the microplate 20 and a plurality of the optical filter parts 31 respectively have light transmission characteristics with respect to the wavelength corresponding to a wavelength selectivity necessary for measuring emission.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供一种能够在诸如测量多色发射等的各种条件下有效测量发射的样品保持构件。 解决方案：样品保持构件2由微孔板20构成，微孔板20具有分别保持样品的多个孔21，其以二维排列状态布置在其上，并且过滤构件30设置成固定在 来自保存在微板20的各个孔21中的来自样品的发射的预定位置发送。 此外，过滤器构件30具有多个排列成二维排列状态的滤光器部件31，以与微板20的多个孔21对应，并且多个滤光器部件31分别具有透光性 相对于对应于测量发射所需的波长选择性的波长的特性。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2007278984A

公开(公告)日：2007-10-25

申请号：JP2006108838

申请日：2006-04-11

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G01N21/64 ,  G01J1/42 ,  G01N21/03

Abstract: PROBLEM TO BE SOLVED: To provide a light measuring device, light measurement method, and light measuring program capable of suppressing the influence due to dust or the like. SOLUTION: A fluorescence measurement apparatus 1, for measuring fluorescence from a sample in a held state by a sample holding member such as a microplate 20, comprises an image acquiring section 40 for acquiring two-dimensional fluorescence images by fluorescence emitted from the sample, and a data analysis device 50 for performing analysis processings of measured data, including the fluorescence image. The fluorescence measuring apparatus extracts a measurement excluding region from the measurement region set for the fluorescence image, by comparing the light intensity distribution in the measurement region of a reference image, acquired under predetermined conditions with a light intensity threshold, that is sets as an analysis region, the region obtained by excluding the measurement excluding region from the measurement region, and performs analysis processings of the fluorescence image. COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供能够抑制灰尘等的影响的光测量装置，光测量方法和光测量程序。 解决方案：一种荧光测量装置1，用于通过诸如微板20的样品保持部件测量来自保持状态的样品的荧光，包括图像获取部40，用于通过从所述微板20发射的荧光获取二维荧光图像 样本和用于对包括荧光图像在内的测量数据进行分析处理的数据分析装置50。 荧光测量装置通过将在预定条件下获取的参考图像的测量区域中的光强度分布与被设置为分析的光强度阈值进行比较，从针对荧光图像设置的测量区域中提取测量排除区域 区域，通过从测量区域排除测量排除区域而获得的区域，并且执行荧光图像的分析处理。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2019020125A

公开(公告)日：2019-02-07

申请号：JP2017135427

申请日：2017-07-11

Applicant: HAMAMATSU PHOTONICS KK

Inventor： SUGIYAMA NORIKAZU ,  MATSUBARA MASANORI ,  YAMAMOTO SATOSHI

IPC: G01N21/64 ,  G02B21/06 ,  G02B21/36

Abstract: 【課題】メンブレンフィルタを通って移動した試料と移動しなかった試料との双方を観察できる試料観察装置及び試料観察方法を提供する。【解決手段】試料観察装置1は、メンブレンフィルタを透過する面状光の照射によって発生した観察光を撮像して蛍光画像データを出力する撮像部13と、第1の試料保持空間に対応する第1の領域と第2の試料保持空間に対応する第2の領域とを特定し、第1の領域に対応する第1の部分画像データと第2の領域に対応する第2の部分画像データとを生成する部分画像生成部と、これらの部分画像データに基づいて第1の観察画像データ及び第2の部分画像データを生成する観察画像生成部と、第1の観察画像データ及び第2の観察画像データに基づいて試料Sの解析を行う解析部と、を備える。【選択図】図1