公开(公告)号：US07491508B2

公开(公告)日：2009-02-17

申请号：US10561201

申请日：2004-06-15

Applicant: Soumitra Roy ,  James M. Wilson

Inventor： Soumitra Roy ,  James M. Wilson

IPC: C12N15/63 ,  C12N15/64 ,  C12N15/861 ,  C12N5/10 ,  A61K48/00 ,  C12P21/00 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/70

CPC classification number: C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/5256 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10344 ,  C12N2710/10351 ,  C12N2760/14134 ,  C12N2810/6018

Abstract: A method for providing an adenovirus from a serotype which does not grow efficiently in a desired cell line with the ability to grow in that cell line is described. The method involves replacing the left and right termini of the adenovirus with the corresponding termini from an adenovirus which grow efficiently in the desired cell line. At a minimum, the left terminus spans the (5′) inverted terminal repeat, the left terminus spans the E4 region and the (3′) inverted terminal repeat. The resulting chimeric adenovirus contains the internal regions spanning the genes encoding the penton, hexon and fiber from the serotype which does not grow efficiently in the desired cell. Also provided are vectors constructed from novel simian adenovirus sequences and proteins, host cells containing same, and uses thereof.

Abstract translation: 描述了从在该细胞系中具有生长能力的所需细胞系中不能有效生长的血清型提供腺病毒的方法。 该方法包括用在期望的细胞系中有效生长的腺病毒的相应末端替换腺病毒的左侧和右侧末端。 至少，左终点跨越（5'）反向末端重复，左终点跨越E4区域，（3'）反向末端重复。 所得到的嵌合腺病毒包含跨越编码来自血清型的五邻体，六邻体和纤维的基因的内部区域，其不能在期望的细胞中有效生长。 还提供了由新型猿猴腺病毒序列和蛋白构成的载体，含有其的宿主细胞及其用途。

公开(公告)号：US07282199B2

公开(公告)日：2007-10-16

申请号：US10423704

申请日：2003-04-25

Applicant: Guangping Gao ,  James M. Wilson ,  Mauricio R. Alvira

Inventor： Guangping Gao ,  James M. Wilson ,  Mauricio R. Alvira

IPC: C12N15/864 ,  C12N15/35 ,  C12N15/63 ,  C12N15/64 ,  C12N7/01 ,  C12P21/00 ,  C07K14/015 ,  C12Q1/70 ,  C12Q1/68

CPC classification number: C12N15/86 ,  A61K9/0019 ,  A61K35/761 ,  A61K38/177 ,  A61K38/45 ,  A61K38/4846 ,  A61K48/00 ,  C07K14/005 ,  C07K14/705 ,  C07K14/755 ,  C12N7/00 ,  C12N9/1018 ,  C12N9/644 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12Y201/03003 ,  C12Y304/21021 ,  C12Y304/21022

Abstract: Sequences of a serotype 8 adeno-associated virus and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles. rAAV8-mediated delivery of therapeutic and immunogenic genes, including Factor VIII, is also provided.

Abstract translation: 提供血清型8腺相关病毒和含有这些序列的载体和宿主细胞的序列。 还描述了在生产rAAV颗粒中使用这种宿主细胞和载体的方法。 还提供rAAV8介导的治疗和免疫原性基因的递送，包括因子VIII。

公开(公告)号：US07186552B2

公开(公告)日：2007-03-06

申请号：US10696900

申请日：2003-10-30

Applicant: James M. Wilson ,  Weidong Xiao

Inventor： James M. Wilson ,  Weidong Xiao

IPC: C12N15/00 ,  C12N15/09 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N5/00 ,  C12N5/02 ,  C12N15/85 ,  C12N15/87

CPC classification number: C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C07K14/505 ,  C07K14/8125 ,  C12N7/00 ,  C12N2750/14042 ,  C12N2750/14121 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2799/025

Abstract: The nucleic acid sequences of adeno-associated virus (AAV) serotype 1 are provided, as are vectors and host cells containing these sequences and functional fragments thereof. Also provided are methods of delivering genes via AAV-1 derived vectors.

Abstract translation: 提供腺相关病毒（AAV）血清型1的核酸序列，载体和含有这些序列及其功能片段的宿主细胞也是如此。 还提供了通过AAV-1衍生的载体递送基因的方法。

公开(公告)号：US07167951B2

公开(公告)日：2007-01-23

申请号：US10263274

申请日：2002-10-02

Applicant: Jerry A. Blades ,  Matthew C. Dewey ,  Bruce A. Thompson ,  David J. Van Maren ,  James M. Wilson

Inventor： Jerry A. Blades ,  Matthew C. Dewey ,  Bruce A. Thompson ,  David J. Van Maren ,  James M. Wilson

IPC: G06F12/00

CPC classification number: G06F3/0664 ,  G06F3/0607 ,  G06F3/0643 ,  G06F3/0679 ,  G06F12/08

Abstract: Mass storage system controller apparatus receives requests for access to files in a data storage system and manages access to the files in the data storage system, the data storage system having a plurality of storage devices and the requests being addressed to the data storage system as a single virtual storage device having addressable Command Regions allocated to controller commands and addressable Mapped Data Regions allocated for data storage. The apparatus includes a file system to manage access to files in the data storage system, the file system having a processor acting as a Command Region processor and acting as a Mapped Data Region processor. A disk emulator responds as a disk drive device to write/read commands and passes the write/read commands addressed to Command Regions of the virtual storage device to the Command Region processor, the write/read commands addressed to Command Regions containing controller commands for the file system. The Command Region processor responds to controller commands in the write/read commands and processes the controller commands to control files in the data storage system. The disk emulator responds as a disk drive device to write/read commands and passes write/read commands addressed to a Mapped Data Region of the virtual storage device to the Mapped Data Region processor. The Mapped Data Region processor responds to a write/read command with a Mapped Data Region address and transfers data to or from the data storage system for files mapped to the Mapped Data Region address.

Abstract translation: 大容量存储系统控制器装置接收访问数据存储系统中的文件的请求，并管理对数据存储系统中的文件的访问，数据存储系统具有多个存储装置，并且请求被作为数据存储系统寻址到数据存储系统 单个虚拟存储设备具有分配给控制器命令的可寻址命令区域和分配用于数据存储的可寻址映射数据区域。 该装置包括用于管理数据存储系统中文件访问的文件系统，该文件系统具有用作命令区域处理器的处理器并用作映射数据区域处理器。 磁盘仿真器作为磁盘驱动器设备作为写入/读取命令进行响应，并将寻址到虚拟存储设备的命令区域的写入/读取命令传递到命令区域处理器，写入/读取命令寻址到包含控制器命令的命令区域 文件系统。 命令区域处理器响应写/读命令中的控制器命令，并处理控制器命令以控制数据存储系统中的文件。 磁盘仿真器作为磁盘驱动器设备进行响应，以写/读命令，并将寻址到虚拟存储设备的映射数据区的写/读命令传递给映射数据区处理器。 映射数据区域处理器使用映射数据区域地址响应写入/读取命令，并将数据传输到数据存储系统或从映射到映射到映射数据区域地址的文件传输数据。

公开(公告)号：US06887463B2

公开(公告)日：2005-05-03

申请号：US10167264

申请日：2002-06-10

Applicant: James M. Wilson ,  Karen Kozarsky ,  Jerome Strauss, III

Inventor： James M. Wilson ,  Karen Kozarsky ,  Jerome Strauss, III

IPC: C12N15/09 ,  A61K35/76 ,  A61K38/00 ,  A61K38/17 ,  A61K48/00 ,  A61P3/06 ,  C07K14/705 ,  C12N5/10 ,  C12N7/00 ,  C12N15/12 ,  C12N15/861 ,  C12N15/86 ,  C12N15/864

CPC classification number: C12N15/86 ,  A61K38/00 ,  A61K48/00 ,  C07K14/705 ,  C12N2710/10344 ,  C12N2799/022

Abstract: The invention provides a recombinant viral vector comprising the DNA of, or corresponding to, at least a portion of the genome of an adenovirus, which portion is capable of infecting a hepatic cell; and a human VLDL receptor gene operatively linked to regulatory sequences directing its expression. The vector is capable of expressing the normal VLDL receptor gene product in hepatic cells in vivo or in vitro. This viral vector is useful in the treatment of metabolic disorders caused by the accumulation of LDL in plasma, such as familial hypercholesterolemia or familial combined hyperlipidemia.

Abstract translation: 本发明提供了一种重组病毒载体，其包含腺病毒基因组的至少一部分的DNA或相当于该部分能够感染肝细胞的DNA; 和与指导其表达的调节序列可操作地连接的人类VLDL受体基因。 该载体能够在体内或体外在肝细胞中表达正常的VLDL受体基因产物。 该病毒载体可用于治疗血浆中LDL积累引起的代谢紊乱，如家族性高胆固醇血症或家族性高脂血症。

公开(公告)号：US06496215B2

公开(公告)日：2002-12-17

申请号：US09739558

申请日：2000-12-15

Applicant: James M. Wilson

Inventor： James M. Wilson

IPC: B41J2700

CPC classification number: G02B26/126 ,  G02B7/1815 ,  G02B26/0825 ,  H04N1/0473 ,  H04N1/1135 ,  H04N1/12 ,  H04N2201/02441 ,  H04N2201/04762 ,  H04N2201/04796

Abstract: A heating element adjusts the curvature of a cylindrical wobble correction mirror to compensate and correct the scan line bow for an optical scanner. A current applied through the heating element along one side of the mirror will cause the mirror to bend vertically for a horizontal beam to adjust the scan line bow for a single beam and approximately equalize the scan line bow for multiple beams.

公开(公告)号：US06483530B2

公开(公告)日：2002-11-19

申请号：US09746898

申请日：2000-12-22

Applicant: James M. Wilson

Inventor： James M. Wilson

IPC: B41J2435

CPC classification number: G02B26/12 ,  B41J2/471 ,  G02B7/00

Abstract: A honeycomb structure is formed integral with a raster output scanning system housing. A constrained layer damper is bonded to the honeycomb structure and the raster output scanning system is mounted on the constrained layer damper. The honeycomb structure and constrained layer damper provide support and reduce vibrations to the raster output scanning system. The cells of the honeycomb structure can be irregular in height, thickness, density or shape to further support and damp vibrations.

Abstract translation: 蜂窝结构与光栅输出扫描系统壳体一体形成。 约束层阻尼器结合到蜂窝结构上，并且光栅输出扫描系统安装在约束层阻尼器上。 蜂窝结构和约束层阻尼器为光栅输出扫描系统提供支撑并减少振动。 蜂窝结构体的细胞在高度，厚度，密度或形状上可能是不规则的，以进一步支撑和减轻振动。

公开(公告)号：US06475769B1

公开(公告)日：2002-11-05

申请号：US09528017

申请日：2000-03-17

Applicant: James M. Wilson ,  Guangping Gao

Inventor： James M. Wilson ,  Guangping Gao

IPC: C12N700

CPC classification number: C12N7/00 ,  A61K48/00 ,  C12N15/86 ,  C12N2510/02 ,  C12N2750/14143 ,  C12N2750/14152

Abstract: Methods for efficient production of recombinant AAV employ a host cell which comprising AAV rep and cap genes stably integrated within the cell's chromosomes, wherein the AAV rep and cap genes are each operatively linked to regulatory sequences capable of directing the expression of the rep and cap gene products upon infection of the cell with a helper virus, a helper gene, and a helper gene product. A method for producing recombinant adeno-associated virus (rAAV) involves infecting such a host cell with a helper virus, gene or gene product and infecting the infected host cell with a recombinant hybrid virus or plasmid vector containing adenovirus cis-elements necessary for replication and virion encapsidation, AAV sequences comprising the 5′ and 3′ ITRs of an AAV, and a selected gene operatively linked to regulatory sequences directing its expression, which is flanked by the above-mentioned AAV sequences.

Abstract translation: 用于有效生产重组AAV的方法使用包含稳定整合在细胞染色体内的AAV rep和cap基因的宿主细胞，其中AAV rep和cap基因各自可操作地连接到能够指导rep和cap基因表达的调控序列 用辅助病毒感染细胞的产物，辅助基因和辅助基因产物。 用于产生重组腺相关病毒（rAAV）的方法包括用辅助病毒，基因或基因产物感染宿主细胞，并用重组杂交病毒或包含复制所需的腺病毒顺式元件的质粒载体感染感染的宿主细胞，以及 病毒体衣壳化，包含AAV的5'和3'ITR的AAV序列，以及选择的基因，其与指导其表达的调控序列有效连接，其侧翼是上述AAV序列。

公开(公告)号：US06428988B1

公开(公告)日：2002-08-06

申请号：US09923726

申请日：2001-08-07

Applicant: James M. Wilson ,  Daniel Phaneuf

Inventor： James M. Wilson ,  Daniel Phaneuf

IPC: C12N1563

CPC classification number: C12N15/86 ,  A61K48/00 ,  C12N9/00 ,  C12N2710/10343 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2810/40 ,  C12N2830/003 ,  C12N2830/42

Abstract: Methods for efficient production of recombinant AAV are described. In one aspect, three vectors are introduced into a host cell. A first vector directs expression of cre recombinase, a second vector contains a promoter, a spacer sequence flanked by loxP sites and rep/cap, and a third vector contains a minigene containing a transgene and regulatory sequences flanked by AAV ITRs. In another aspect, the host cell stably or unducibly expresses cre recombinase and two vectors carrying the other elements of the system are introduced into the host cell.

Abstract translation: 描述了用于有效生产重组AAV的方法。 在一个方面，将三个载体引入宿主细胞。 第一载体指示cre重组酶的表达，第二载体包含启动子，侧翼为loxP位点和rep / cap的间隔序列，第三载体含有含有转基因的小基因和侧翼为AAV ITR的调控序列。 在另一方面，宿主细胞稳定地或不可约地表达cre重组酶，并且携带系统的其它元件的两个载体被引入宿主细胞。

公开(公告)号：US06387368B1

公开(公告)日：2002-05-14

申请号：US09546738

申请日：2000-04-11

Applicant: James M. Wilson ,  William M. Kelley ,  Krishna J. Fisher

Inventor： James M. Wilson ,  William M. Kelley ,  Krishna J. Fisher

IPC: A61K4800

CPC classification number: C12N15/86 ,  A61K48/00 ,  A61K2121/00 ,  C12N7/00 ,  C12N2710/10344 ,  C12N2750/14143 ,  C12N2750/14151

Abstract: The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5′ and 3′ ITR sequences from an AAV, and a selected transgene. Other hybrid vectors form a polycation conjugate and incorporate an AAV rep gene in a single particle. These hybrid virus vectors are characterized by high titer transgene delivery to a host cell and the ability to stably integrate the transgene into the host cell chromosome. Also disclosed is the use of the hybrid vectors to produce large quantities of recombinant AAV.

Abstract translation: 本发明提供了一种杂合载体构建体，其包含一部分腺病毒，来自AAV的5'和3'ITR序列以及选择的转基因。 其他杂合载体形成聚阳离子缀合物并将AAV rep基因并入单个颗粒中。 这些杂种病毒载体的特征在于高滴度转移至宿主细胞的转基因，以及将转基因稳定整合入宿主细胞染色体的能力。 还公开了杂交载体用于产生大量重组AAV的用途。