ALKALINE PULLULANASE, MICROORGANISM PRODUCING THE SAME, AND PROCESS FOR PRODUCING THE SAME

    公开(公告)号:CA2024194A1

    公开(公告)日:1991-03-01

    申请号:CA2024194

    申请日:1990-08-29

    Applicant: KAO CORP

    Abstract: A novel alkaline pullulanase; a microorganism producing the alkaline pullulanase; and a process for producing the alkaline pullulanase are disclosed. The alkaline pullulanase of the present invention has a higher optimum pH range (pH 9.5-11) than conventional alkaline pullulanases and shows excellent stability in a wider pH range. It has also an optimum temperature of higher than 50 DEG C and is thermally stable up to 40 DEG C. The alkaline pullulanase has also strong resistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. The alkaline pullulanase can advantageously be used as a detergent component. Also, it can be utilized for producing many kinds of oligosaccharides and also together with alpha -amylase for producing various monosaccharides.

    Modified promoter
    37.
    发明专利

    公开(公告)号:AU2010308865A1

    公开(公告)日:2012-05-17

    申请号:AU2010308865

    申请日:2010-10-18

    Applicant: KAO CORP

    Abstract: The present invention provides a modified promoter, an expression vector and a transformant each containing the promoter, and a method for producing a gene product of interest using the transformant. The invention provides a modified promoter, including a nucleotide sequence of a promoter derived from bacterium belonging to the genus Bacillus in which at least one nucleotide sequence selected from the following has been modified: a nucleotide sequence represented by SEQ ID NO: 1; a nucleotide sequence equivalent to the nucleotide sequence represented by SEQ ID NO: 1, except that one or a plurality of bases therein are substituted, deleted, added or inserted; and a nucleotide sequence having a sequence identity of 70% or more with respect to the nucleotide sequence represented by SEQ ID NO: 1.

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