公开(公告)号：EP2807478A1

公开(公告)日：2014-12-03

申请号：EP13702546.6

申请日：2013-01-18

Applicant: Life Technologies Corporation ,  Bustillo, James ,  Rearick, Todd ,  Hinz, Wolfgang ,  Fife, Keith

Inventor： BUSTILLO, James ,  REARICK, Todd ,  HINZ, Wolfgang ,  FIFE, Keith

IPC: G01N27/414

CPC classification number: B01L3/5027 ,  B01L3/502707 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/0893 ,  G01N27/4145

Abstract: A method of fabricating a microwell in an array structure is disclosed herein. The array structure can include a plurality of field effect transistors (FETs), where each FET has a gate structure. The method can include disposing a titanium nitride (TiN) layer on at least one conductive layer coupled to the gate structure of at least one FET. A insulation layer can also be disposed on the array structure, where the insulation layer lies above the TiN layer. Further, an opening above the gate structure of the at least one FET can be etched to remove the insulation layer above the gate structure and to expose the TiN layer. A microwell with at least one sidewall formed from the insulation layer and with a bottom surface formed from the TiN layer is a result of the etching process.

公开(公告)号：EP2609476A1

公开(公告)日：2013-07-03

申请号：EP11820540.0

申请日：2011-08-23

Applicant: Life Technologies Corporation

Inventor： REARICK, Todd ,  JORDAN, Jeremy ,  NOBILE, John ,  MILESKI, William, Julian ,  HO, Louie

IPC: G05D23/20 ,  G01N31/00 ,  G01K11/00

CPC classification number: G01N27/4145 ,  B01L3/502 ,  B01L3/52 ,  B01L7/00 ,  B01L2200/0605 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/0627 ,  B01L2300/0829 ,  B01L2300/1805 ,  B01L2300/1894 ,  B01L2400/0487 ,  B01L2400/0622 ,  G01N27/414 ,  G01N31/20 ,  G05D23/19 ,  G05D23/1931

Abstract: An apparatus for detecting chemical reactions may be provided. The apparatus may comprise a chemical detection device. The chemical detection device may include a chemical sensor, which may be mounted on the chemical detection device. The apparatus may further comprise a valve block. The valve block may fluidly couple a plurality of reagent containers to the chemical detection device. The apparatus may further comprise a heat exchanger and a controller. The controller may control a fluid connection between the valve block and the chemical detection device. The controller may be also configured to adjust a temperature of a selected reagent from the plurality of reagent containers via the heat exchanger. The temperature of the selected reagent may be adjusted prior to the reagent entering the chemical detection device.

Abstract translation: 可以提供用于检测化学反应的装置。 该装置可以包括化学检测装置。 化学检测装置可以包括可安装在化学检测装置上的化学传感器。 该装置还可以包括阀块。 阀块可以将多个试剂容器流体耦合到化学检测装置。 该装置还可以包括热交换器和控制器。 控制器可以控制阀块和化学检测装置之间的流体连接。 控制器还可以被配置为经由热交换器从多个试剂容器调节所选择的试剂的温度。 可以在试剂进入化学检测装置之前调整所选择的试剂的温度。

公开(公告)号：EP2435128A2

公开(公告)日：2012-04-04

申请号：EP10780930.3

申请日：2010-05-27

Applicant: Life Technologies Corporation

Inventor： ROTHBERG, Jonathan M. ,  HINZ, Wolfgang ,  DAVIDSON, John F. ,  VAN OIJEN, Antoine M. ,  LEAMON, John H. ,  HUBER, Martin ,  BUSTILLO, James M. ,  MILGREW, Mark, James ,  SCHULTZ, Jonathan ,  MARRAN, David ,  REARICK, Todd ,  JOHNSON, Kim L.

IPC: A61M37/00 ,  A61B5/145

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/27 ,  G01N27/414 ,  Y10T29/49002

Abstract: Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.