公开(公告)号：JP2007215472A

公开(公告)日：2007-08-30

申请号：JP2006039184

申请日：2006-02-16

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： MATSUO YUICHIRO ,  KAWAI TAKESHI ,  SHIMADA YOSHIHIRO

IPC: C12M1/22 ,  C12M3/00 ,  C12M3/06

CPC classification number: C12M23/10 ,  C12M23/34 ,  C12M25/02 ,  C12M27/22

Abstract: PROBLEM TO BE SOLVED: To provide a cell culture container which enables the free selection of a timing and amount for supplying a biochemical substance or the like secreted from one kind of cells in a culture process to the other kind of cells, and thereby facilitating finer observation of the culture.
SOLUTION: This cell culture container is characterized by disposing a dividing wall 2b for approximately horizontally dividing culture regions X, Y for culturing the cells A, B therein in the culture regions X, Y, disposing passages 3 permitting the pass of a culture solution 5 in the dividing wall 2b, and disposing filter members 4 permitting the pass of the culture solution 5 but inhibiting the pass of the cells A, B and an opening or closing means 6 for opening or closing the pass 3 in the dividing wall 2b.
COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 待解决的问题：提供一种细胞培养容器，其能够自由选择将培养过程中从一种细胞分泌的生物化学物质等供给另一种细胞的时间和量，以及 从而促进对文化的更好的观察。 解决方案：该细胞培养容器的特征在于在培养区域X，Y中设置用于大致水平分割用于培养细胞A，B的培养区域X，Y的分隔壁2b， 分离壁2b中的培养液5，并且设置允许培养液5通过但阻止细胞A，B通过的过滤器部件4和用于打开或关闭分隔壁中的通过部3的打开或关闭装置6 2B。 版权所有（C）2007，JPO＆INPIT

公开(公告)号：JP2019003027A

公开(公告)日：2019-01-10

申请号：JP2017117413

申请日：2017-06-15

Applicant: OLYMPUS CORP

Inventor： SHIMADA YOSHIHIRO

IPC: G02B21/36 ,  G01N21/13 ,  G01N21/64

Abstract: 【課題】サンプルの全域を観察でき、コンタミネーションの心配がなく、スペース効率を向上して多くのサンプルを観察可能に収容すること。【解決手段】少なくとも一部が光学的に透明な側壁部15bおよび少なくとも一部が光学的に透明な底面部15cを有し、サンプルSを収容可能な複数のキュベット15と、これら複数のキュベット15を、深さ方向に交差する一方向に透明な各側壁部15bを露出させるように、各底面部15cの位置を深さ方向にずらしながら前記一方向に沿って直線状に配列させて支持する連結部17とを備えるマイクロプレート3を提供する。【選択図】図1

公开(公告)号：JP2015007661A

公开(公告)日：2015-01-15

申请号：JP2013131824

申请日：2013-06-24

Applicant: オリンパス株式会社 ,  Olympus Corp

Inventor： SHIMADA YOSHIHIRO ,  UENO MAKIO ,  TAMANO SHINGO ,  HAYASHI NAOKI ,  YOKOI EIJI

IPC: G02B21/06 ,  G02B21/36

CPC classification number: G02B21/0032 ,  G02B21/002 ,  G02B21/0036 ,  G02B21/0096 ,  G02B21/06 ,  G02B21/26 ,  G02B27/4261

Abstract: 【課題】光変調素子で光強度を変化させても、光学系の瞳の中心に対する照明光の中心が、光強度の変更前と変更後とで変化しない走査型光学顕微鏡を提供する。【解決手段】照明光を射出する光源と、照明光を試料に照射する集光光学系と、試料を載置するステージと、照明光と前記ステージとを相対的に変位させる走査装置と、試料からの光を集光する検出光学系と、試料からの光を検出する光検出器と、を備え、光源よりも集光光学系側に、光変調素子と、リレー光学系と、が配置され、光変調素子には、振幅のみを変化させた変調信号が入力され、所定の振幅の変調信号に対して、光変調素子から射出する照明光が集光光学系の光軸と一致するように、光変調素子は位置決めされており、集光光学系の瞳の位置と光変調素子の位置とが、少なくともリレー光学系を介して共役になっていることを特徴とする。【選択図】図1

Abstract translation: 要解决的问题：为了提供一种扫描光学显微镜，即使在光调制元件的光强度变化时，能够防止在改变光强度之前和之后相对于光学系统的光瞳的中心的照明光的中心变化 解决方案：扫描光学显微镜包括：发射照明光的光源; 聚焦光学系统用照明光照射样品; 安装样品的阶段; 相对移动照明光和舞台的扫描装置; 检测光学系统，聚焦来自样品的光; 以及检测来自样品的光的光电检测器。 光调制元件和中继光学系统相对于光源设置在聚焦光学系统附近。 仅将其幅度改变的调制信号输入到光调制元件。 光调制元件被定位成使得从光调制元件发射的照明光相对于调制信号以预定幅度与聚焦光学系统的光轴相匹配。 至少在中继光学系统上，聚焦光学系统和光调制元件的光瞳的位置处于共轭关系。

公开(公告)号：JP2011112394A

公开(公告)日：2011-06-09

申请号：JP2009266658

申请日：2009-11-24

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TANIGAWA YOSHIHISA ,  OBA MASAHIRO ,  SHIMADA YOSHIHIRO

IPC: G01N21/64 ,  G01N21/76 ,  G01N21/78

CPC classification number: G01J3/02 ,  G01J3/0208 ,  G01J3/4406 ,  G01N21/6456 ,  G01N21/763 ,  G02B13/22 ,  G02B21/16

Abstract: PROBLEM TO BE SOLVED: To provide a low-cost and miniaturized imaging device for a low-light sample capable of performing fluorescence observation and emission observation, without changing the position of a sample using a single device and using a general cooled CCD.
SOLUTION: In the imaging device for a low-light sample equipped with an image forming optical system 1, having an object lens 11 and an image forming lens 12 for forming the sample image of a sample 10, having a spot light source for emitting a low light containing fluorescence, an illumination optical system 2 for irradiating the sample with the light from an illumination light source to emit fluorescence and an imaging means 3 for photographing an image having a large number of pixels and corresponding to the sample image, the illumination optical system is constituted so that the light from the illumination light source irradiates the sample, without going via the object lens and the image forming optical system is substantially telecentric and equipped with a filter 13, which extracts the fluorescence from the sample in a wavelength selective manner, between the object lens and the image forming lens and isconstituted so as to condense the weak light from the spot light source and form an aery disk having a size which is substantially the same as that of a pixel or smaller than that of the pixel.
COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 要解决的问题：为了提供能够进行荧光观察和发射观测的低光样品的低成本和小型化的成像装置，而不使用单个装置改变样品的位置并使用通用的冷却CCD 。 解决方案：在具有成像光学系统1的具有物镜11和成像透镜12的用于形成样品10的样品图像的低光样品的成像装置中，具有点光源 为了发射含有荧光的低光，照明光学系统2用于用来自照明光源的光照射样品以发射荧光;以及成像装置3，用于拍摄具有大量像素且对应于样本图像的图像， 照明光学系统被构造成使得来自照明光源的光不经过物镜照射样品，并且成像光学系统基本上是远心的，并且装备有过滤器13，滤光器13从样品中提取荧光 在物镜和图像形成透镜之间进行波长选择的方式，并且被构造成使来自聚光源a的弱光 nd形成具有与像素的尺寸基本相同的尺寸或小于像素的尺寸的大小的圆盘。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2010256077A

公开(公告)日：2010-11-11

申请号：JP2009104025

申请日：2009-04-22

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： SHIBAZAKI TAKAMI ,  SHIMADA YOSHIHIRO

IPC: G01N21/64

Abstract: PROBLEM TO BE SOLVED: To provide a cell image acquiring device for acquiring an almost focused image even with respect to an imaging field failed in focusing in scanning of the whole of a well, and a cell image acquiring method. SOLUTION: The cell image acquiring device includes an imaging device equipped with an object lens 2, a feed device 3 for feeding the well to an imaging position, a device 4 for detecting the focus matching state of the target in the well at the imaging position, and a focus matching device for adjusting the focus of the object lens on the basis of the detected focus matching state. The imaging device divides the well into a plurality of imaging fields to image the same and the feed device 3 successively feeds the well so that the imaging fields spirally move to the outside from the center of the well. The device 4 detects a focal position at each imaging field to store the focal position succeeded in detection in a memory region and extracts the focal position of the most vicinal imaging field succeeded in the detection of the focal position from the memory region toward the center of the well in a case failed in the detection of the focal position. The focus matching device matches the focus of the object lens with the extracted focal position of the imaging field. COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 要解决的问题：提供一种用于获取即使相对于在整个井的扫描中聚焦的成像场失真的几乎聚焦的图像的单元图像获取装置，以及单元图像获取方法。 解决方案：小区图像获取装置包括配备有物镜2的成像装置，用于将孔馈送到成像位置的进给装置3，用于检测井中的目标的聚焦匹配状态的装置4 成像位置，以及用于基于检测到的焦点匹配状态来调整物镜的焦点的聚焦匹配装置。 成像装置将井分成多个成像场以对其进行成像，并且进给装置3连续地进给井，使得成像场从井的中心螺旋地移动到外部。 设备4检测每个成像领域的焦点位置以将检测中的焦点位置存储在存储器区域中，并且提取从存储区域向中心位置检测焦点位置的最邻近成像场的焦点位置 在这种情况下，井中的焦点位置检测失败。 焦点匹配装置将物镜的焦点与提取的成像场焦点位置相匹配。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2009282357A

公开(公告)日：2009-12-03

申请号：JP2008135110

申请日：2008-05-23

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： SHIBAZAKI TAKAMI ,  TAKAGI KOSUKE ,  SHIMADA YOSHIHIRO

IPC: G02B7/28 ,  G02B21/24

Abstract: PROBLEM TO BE SOLVED: To provide an observation apparatus capable of aligning the focusing position to an observation position in a short time even when an objective lens inapplicable to automatic focus detection of active type is used, and to provide an observation method.
SOLUTION: The observation apparatus comprises an objective lens 1 to be focused on a predetermined reference surface in a vessel 11, an objective lens 2 for observing a predetermined observation position 10a, a means 3 capable of switching the objective lenses 1 and 2, an automatic focusing means 4 for performing the automatic focus detection of active type and matching the focusing position of the objective lens 1 with the reference surface, a position information detecting means 5 for detecting relative position information in the optical axis direction with the objective lens 1 on the reference surface when the objective lens 1 is focused on the reference surface, and an automatic focusing means 6 for adjusting the relative distance with the vessel in the objective lens 2 so that the focusing position of the objective lens 2 matches with the observation position using the position information and the relative position information in the optical axis direction with the focusing position of the objective lens 2 at the focusing position of the objective lens 1.
COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：即使当使用不适用于活性型自动聚焦检测的物镜时，也能够在短时间内将聚焦位置与观察位置对准的观察装置，并提供观察方法。 解决方案：观察装置包括要聚焦在容器11中的预定参考表面上的物镜1，用于观察预定观察位置10a的物镜2，能够切换物镜1和2的装置3 ，用于执行有源类型的自动聚焦检测并将物镜1的聚焦位置与参考表面匹配的自动对焦装置4，用于利用物镜检测光轴方向的相对位置信息的位置信息检测装置5 1，在物镜1聚焦在基准面上时，在基准面上具有1个自由聚焦装置6，用于调节与物镜2中的容器的相对距离，使得物镜2的聚焦位置与观察 使用位置信息和光轴方向上的相对位置信息与聚焦波段的位置 在物镜1的聚焦位置处的物镜2的位置。版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2008064534A

公开(公告)日：2008-03-21

申请号：JP2006241312

申请日：2006-09-06

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  SHIMADA YOSHIHIRO ,  MATSUO YUICHIRO

IPC: G01N33/48 ,  C12M1/34 ,  G06T1/00

CPC classification number: G01N15/1463 ,  G06K9/0014 ,  G06T7/0012 ,  G06T2207/30024

Abstract: PROBLEM TO BE SOLVED: To separate and extract a cell showing specific properties from noise by processing a cell image to analyze the same.
SOLUTION: This cell image processor 1 is equipped with a feature quantity measuring part 11 for processing the cell image acquired by photographing the cells and measuring the feature quantities of the cells contained in the cell image, a feature quantity distribution forming part 12 for forming the distribution of the measured feature quantities, a group forming part 13 for grouping the cells having the continuously distributed feature quantities in the formed distribution of the feature quantities and a specific cell extraction part 14 for extracting the cell having a predetermined range of the feature quantities at both ends of distribution of the feature quantities of the respective groups as a specific cell.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：通过处理细胞图像来分析并提取显示特定性质的细胞从噪声中分析。 解决方案：该单元图像处理器1配备有特征量测量部11，用于处理通过拍摄单元获取的单元图像并测量单元图像中包含的单元的特征量，特征量分布形成部12 为了形成所测量的特征量的分布，组形成部分13用于将具有连续分布特征量的细胞分组在特征量的形成分布中，以及特定细胞提取部分14，用于提取具有预定范围的特征量的细胞 作为特定单元的各组的特征量的分布的两端的特征量。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2004070371A

公开(公告)日：2004-03-04

申请号：JP2003322166

申请日：2003-09-12

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： SHIMADA YOSHIHIRO

IPC: G01N21/64 ,  G02B21/00

Abstract: PROBLEM TO BE SOLVED: To provide a scanning type optical microscope which can guide fluorescent light to a photodetector without lowering the confocal effect and without causing a loss of the fluorescent light for respective wavelengths of the fluorescent light on the multiple dying.
SOLUTION: The scanning type optical microscope scanning a sample with a laser beam is provided with spectral resolution means 11a, 11b which separates the fluorescent light from the sample, wavelength region splitting means 12a∼12c which split the fluorescent light separated by the spectral resolution means to a plurality of wavelength regions, a plurality of imaging optical systems 14a∼14d which are respectively disposed on respective optical paths split into a plurality of wavelength regions and subject the fluorescent light from the sample to imaging, a plurality of confocal diaphragms 15a∼15d which are respectively disposed on the respective optical paths and are arranged on focal positions of the imaging optical systems and a plurality of photodetectors 16a∼16d which are respectively disposed on the respective optical paths and detect the fluorescent light from the sample passing through the confocal diaphragms.
COPYRIGHT: (C)2004,JPO

Abstract translation: 要解决的问题：提供一种扫描型光学显微镜，其可以在不降低共焦效应的情况下将荧光引导到光电检测器，并且在多次垂死时不会引起荧光的各波长的荧光损失。 解决方案：用激光束扫描样品的扫描型光学显微镜设置有分离荧光从样品的光谱分辨装置11a，11b，波长区域分离装置12a〜12c，其分离由 光谱分辨率意味着多个波长区域，多个成像光学系统14a〜14d分别设置在分离成多个波长区域的各光路上，并使来自样品的荧光受到成像，多个共焦膜片 15a〜15d分别设置在各光路上并且配置在成像光学系统的焦点位置和分别设置在各光路上的多个光电检测器16a〜16d，并检测来自样品的荧光 共聚圈。 版权所有（C）2004，JPO