中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

63 records (took 0.028 seconds)

    Preparation of stable enzymatic reagent
    32.
    发明专利
    Preparation of stable enzymatic reagent 失效
    稳定酶试剂的制备

    公开(公告)号：JPS59166084A

    公开(公告)日：1984-09-19

    申请号：JP4119283

    申请日：1983-03-11

    Applicant: Hitachi Chem Co Ltd ,  Oriental Yeast Co Ltd

    Inventor： TANNO KAZUNOBU ,  OOMORI ITOSHI ,  OKA OSAMU ,  SUZUKI YASUO

    IPC: C12N9/96 ,  C12Q1/00

    Abstract: PURPOSE: To reduce deactivation of enzyme during preservation, by adding modified gelatin to an enzyme-containing solution, lyophilizing it.
    CONSTITUTION: Gelatin is heat-treated at 80W120°C for 5W20min, to give modified gelatin. The modified gelatin has 5,000W1,000 average mol.wt. The modified gelatin is added to an enzyme-containing solution in such a way that powder prepared after lyophilization contains 5W80wt% (especially 10W60wt%) modified gelatin, and it is melted at a temperature not to deactivate the enzyme. It is then lyophilized by a conventional procedure.
    COPYRIGHT: (C)1984,JPO&Japio

    Abstract translation: 目的：为了减少保存期间酶的失活，通过向含酶溶液中加入改性明胶，将其冻干。 构成：明胶在80-120℃下热处理5-20分钟，得到改性明胶。 改性明胶具有5,000-1,000平均摩尔重量 将修饰的明胶加入到含酶溶液中，使冻干后制备的粉末含有5-80重量％（特别是10-60重量％）的改性明胶，并在不使酶失活的温度下熔化。 然后通过常规方法冻干。

    QUANTITATIVE ANALYZER WITH IMMOBILIZED OXIDASE
    33.
    发明专利
    QUANTITATIVE ANALYZER WITH IMMOBILIZED OXIDASE 失效

    公开(公告)号：JPS55131383A

    公开(公告)日：1980-10-13

    申请号：JP3630579

    申请日：1979-03-29

    Applicant: ORIENTAL YEAST CO LTD ,  TOYO JOZO KK

    Inventor： UTSUKI YOSHIO ,  FUJINO SATOMI ,  OKA OSAMU ,  NAITOU TOSHIKUNI ,  IZUMI ROKUROU ,  MATSUMOTO KUNIO

    IPC: C12M1/40 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/54 ,  G01N27/28 ,  G01N27/416

    Abstract: PURPOSE:To analyze glucose or cholesterol quantitatively with accuracy, without reaction disturbance due to air, etc., by maintaining the temperature of a buffer bath higher than that of the reaction vessel. CONSTITUTION:The buffer 6, maintained at a temperature about 5-10 deg.C higher than that of the reaction vessel 16 by the heater 2 of the buffer bath 1, is sent to the sample injection unit 8 by the transfer pump 7. A given amount of a sample, e.g., serum, is injected by the syringe 10 into the buffer 16, which is then passed from the pipe 11 through three-way cock 14 to the vessel 16 at about 25-40 deg.C in the thermostatic chamber 20, and reacted with the immobilized oxidase set therein. After completion of the reaction, the buffer 16 is passed from the pipe 17 to the oxygen analyzer 18 to determine the consumed oxygen and amount of glucose and cholesterol. The liquid after the determination is passed from the pipe 19 through the three-way cock 15, and discharged from the pipe 13.

    UREA DETERMINATION REAGENT
    36.
    发明专利
    UREA DETERMINATION REAGENT 失效

    公开(公告)号：JP2000041699A

    公开(公告)日：2000-02-15

    申请号：JP14151499

    申请日：1999-05-21

    Applicant: ORIENTAL YEAST CO LTD

    Inventor： MATSUKAWA HIROKAZU ,  OKA OSAMU ,  FUJITA TAKESHI

    IPC: C12N15/09 ,  C12N9/80 ,  C12Q1/58

    Abstract: PROBLEM TO BE SOLVED: To obtain a urea determination reagent for subjecting urea derived from a biological substance to a rating assay by using a urease reaction as a rate-determining step without highly diluting the sample by using a variant type urease having reduced affinity for urea. SOLUTION: A natural urease gene derived from Klebsiella aerogenes is subjected to site-directed mutagenesis so that histidine at the amino acid sequence α 219th of urease is mutated into alanine, asparagine, glutamine, etc., to give a variant type urease having reduced affinity for urea. The variant type urea having reduced affinity for urea is used to give the objective urea determination reagent capable of determing urea derived from a biological substance by using a urease reaction as a rating assay without highly diluting the sample and without adding a substance for regulating the affinity of enzyme to a substrate by directly suppressing urease.

    Diaphragm type gaseous carbonic acid sensor
    39.
    发明专利
    Diaphragm type gaseous carbonic acid sensor 失效
    透膜型气体碳酸传感器

    公开(公告)号：JPS5965752A

    公开(公告)日：1984-04-14

    申请号：JP17624682

    申请日：1982-10-08

    Applicant: Oriental Yeast Co Ltd

    Inventor： SUZUKI ATSUO ,  OKA OSAMU ,  OOHASHI MINORU

    IPC: G01N27/404 ,  G01N27/416

    CPC classification number: G01N27/4162

    Abstract: PURPOSE:To obtain a diaphragm type gaseous carbonic acid sensor excellent in sensitivity and stability and capable of calibrating by a pH buffer solution in good reproducibility, by using a reticulate nonwoven cloth as a spacer between a gas selectively permeable diaphragm and an electrode membrane. CONSTITUTION:A reticulate nonwoven cloth spacer 2 is attached between a gas selectively permeable diaphragm 1 of a sensor consisting of an outer cylindrical pipe 5 providing with a gas selectively permeable diaphragm 1 at the one end, a composite pH electrode 6, and an inner liquid 7 and a composite pH electrode 6 by a spacer band 4. Synthetic fibers such as polyamide, polyester etc. are used for materials of the reticulate nonwoven cloth spacer but hydrophillic materials such as cotton, rayon etc. are preferable from a viewpoint of being accustomed to an electrolyte. The reticulate nonwoven fabric has a good permeability to the inner liquid and gaseous carbonic acid is diffused easily and also, an output is stable. Further, sensitivity is good and meshes are not disturbed by detaching and attaching the space and also, the reproducibility of measured value is good.

    Abstract translation: 目的：通过使用网状无纺布作为气体选择性渗透膜片和电极膜之间的间隔物，获得灵敏度和稳定性优异的隔膜式气态碳酸传感器，能够通过pH缓冲溶液以良好的再现性进行校准。 构成：将网状无纺布间隔件2安装在传感器的气体选择性透过膜1上，该传感器由一端设有气体选择性透过膜1的外筒管5，复合pH电极6和内液 7和通过间隔带4的复合pH电极6.合成纤维如聚酰胺，聚酯等用于网状非织造布间隔物的材料，但是从习惯上的观点来看，优选亲水性材料如棉，人造丝等 到电解液。 网状无纺布对内部液体具有良好的渗透性，气态碳酸容易扩散，输出稳定。 此外，灵敏度好，并且不会通过分离和附着空间来干扰网格，并且测量值的重现性良好。

    STABILIZING DEVICE FOR BUFFER SOLUTION
    40.
    发明专利
    STABILIZING DEVICE FOR BUFFER SOLUTION 失效

    公开(公告)号：JPS5830666A

    公开(公告)日：1983-02-23

    申请号：JP12867381

    申请日：1981-08-19

    Applicant: ORIENTAL YEAST CO LTD

    Inventor： NUMAZAWA KENICHI ,  OKA OSAMU ,  YOKOYAMA TADASHI ,  NAITOU TOSHIKUNI

    IPC: G01N33/49 ,  A61M5/44 ,  F24H1/00 ,  G01N33/48 ,  G01N35/00 ,  G01P13/00

    Abstract: PURPOSE:To raise safety by installing a device that detects the flow of a buffer solution and cutting off the power source to temperature regulating device and so on or sounding an alarm buzzer or other safety means. CONSTITUTION:A buffer solution 16 in a vessel 15 for the buffer solution is supplied by means of a circulation pump 25 through a pipe 17 to a pipe 27 that is wound round a heating drum 26. The buffer solution whose temperature has reached a constant temperature through the heating drum 26 is returned again to the buffer solution vessel 15 by traversing the wall. Here the oxygen dissolved in the buffer solution is balanced by the multiple effect of the negative effect to the buffer solution and pulsating flow effect by the circulation pump 25 and the heating effect by the heating drum 26. While the buffer solution is circulating, the light flux from the light source 19 gets into a light receiving element 21 to turn on a switching circuit and supplies electric power of the power source 24. If the buffer solution is exhausted, total reflection develops by the difference of the reflective indices by passing from a glass tube 20' to the air and incident light to the light receiving element 21 to turn off the switching circuit and overheat is avoided.

Patent Agency Ranking