公开(公告)号：EP2432579A2

公开(公告)日：2012-03-28

申请号：EP10749681.2

申请日：2010-05-10

Applicant: Stokes Bio Limited

Inventor： DAVIES, Mark ,  DALTON, Tara

IPC: B01F13/00 ,  B01F15/02 ,  B01F5/04 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  F04B43/04 ,  G01N1/34 ,  G01N35/00 ,  G01N35/08 ,  G01N33/50

CPC classification number: B01L3/5025 ,  B01F13/0071 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01J2219/00889 ,  B01L3/502715 ,  B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2300/0819 ,  B01L2300/0838 ,  B01L2300/0867 ,  B01L2300/1805 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/0688 ,  C40B50/08 ,  C40B60/14 ,  G01N2035/00544

Abstract: The present invention generally relates to methods of constructing liquid bridges and methods of forming predetermined combinations of samples using liquid bridges.

公开(公告)号：EP1981624B1

公开(公告)日：2011-09-07

申请号：EP07705995.4

申请日：2007-02-07

Applicant: Stokes Bio Limited

Inventor： DAVIES, Mark ,  DALTON, Tara ,  CURRAN, Kieran

IPC: B01F13/00 ,  B01L3/00 ,  G01N35/08

CPC classification number: B01L3/502784 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01F2215/0037 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502746 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2300/0838 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/082 ,  C12Q1/686 ,  F17D1/08 ,  Y10T137/87676 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575

Abstract: A bridge (30) comprises a first inlet port (31) at the end of a capillary, a narrower second inlet port (32) which is an end of a capillary, an outlet port (33) which is an end of a capillary, and a chamber (34) for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (34). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary- suspended spherical form upon entering the chamber (34). The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port (32) causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (32), and the droplets combine at the liquid bridge (30). In another embodiment, a droplet (55) segments into smaller droplets which bridge the gap between the inlet and outlet ports.

公开(公告)号：EP2069070A1

公开(公告)日：2009-06-17

申请号：EP07805479.8

申请日：2007-09-27

Applicant: Stokes Bio Limited

Inventor： DAVIES, Mark ,  DALY, John ,  DALTON, Tara

IPC: B01L7/00 ,  C12Q1/68 ,  G01N35/00

CPC classification number: B01L7/525 ,  B01L3/508 ,  B01L7/54 ,  B01L2300/0654 ,  B01L2300/0809 ,  B01L2300/1827 ,  B01L2400/0442 ,  B01L2400/0445 ,  G01N21/6428 ,  G01N2021/6439 ,  G01N2201/062

Abstract: An apparatus (1) is for DNA amplification with quantitative measurements. A biological sample is held in a cell (2) for the amplification, the cell (2) defining a single space within which the sample rotates. On one side a copper heater (3) is located to supply heat to the cell (2), and on the other side there is a cooling copper block (4) withdrawing heat from the cell. The locations of the heater (3) and the cooling block (4) generate a natural convection loop internally within the cell (2) without need for active cooling - the block (4) passively cooling by withdrawing heat from the direction of the heater (3).A detector (9, 27) captures readings in real time and a processor (10) generates an S-curve for change of sample emission with time. The S-curve (Figs. 4 and 5) also includes a thermal cycle number corresponding to the time parameter, so that the S-curve is given in the traditional qPCR intensity vs. cycle number.

公开(公告)号：EP1991357A1

公开(公告)日：2008-11-19

申请号：EP07705997.0

申请日：2007-02-07

Applicant: Stokes Bio Limited

Inventor： DAVIES, Mark ,  DALTON, Tara

IPC: B01L7/00 ,  B01L3/00 ,  G01N35/08

CPC classification number: B01L7/525 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0838 ,  B01L2300/0867 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/185 ,  B01L2400/0487 ,  C12Q1/686 ,  G01N21/6428 ,  G01N2201/0826 ,  G01N2201/0833

Abstract: A thermal cycling device ( 3 ) device a number of fixed thermal zones ( 11, 12, 13 ) and a fixed conduit ( 10 ) passing through the thermal zones. A controller maintains each thermal zone including its section of conduit ( 10 ) at a constant temperature. A series of droplets flows through the conduit ( 10 ) so that each droplet is thermally cycled, and a detection system detects fluorescence from droplets at all of the thermal cycles. The conduit is in a single plane, and so a number of thermal cycling devices may be arranged together to achieve parallelism. The flow conduit comprises a channel ( 17 ) and a capillary tube ( 10 ) inserted into the channel. The detection system may perform scans along a direction to detect radiation from a plurality of cycles in a pass.

公开(公告)号：EP1981625A1

公开(公告)日：2008-10-22

申请号：EP07705996.2

申请日：2007-02-07

Applicant: Stokes Bio Limited

Inventor： DAVIES, Mark ,  DALTON, Tara ,  GARVEY, Julie ,  CURRAN, Kieran ,  CURTIN, Damian

IPC: B01F13/00 ,  B01L3/00 ,  G01N35/08

CPC classification number: B01J19/0046 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01J2219/00313 ,  B01J2219/00353 ,  B01J2219/00479 ,  B01J2219/00587 ,  B01J2219/00608 ,  B01L3/5025 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2400/0487 ,  Y10T436/25

Abstract: A multi-port liquid bridge (1) adds aqueous phase droplets (10) in an enveloping oil phase carrier liquid (11) to a draft channel (4, 6). A chamber (3) links four ports, and it is permanently full of oil (11) when in use. Oil phase is fed in a draft flow from an inlet port (4) and exits through a draft exit port (6) and a compensating flow port (7). The oil carrier and the sample droplets (3) ('aqueous phase') flow through the inlet port (5) with an equivalent fluid flow subtracted through the compensating port (7). The ports of the bridge (1) are formed by the ends of capillaries held in position in plastics housings. The phases are density matched to create an environment where gravitational forces are negligible. This results in droplets (10) adopting spherical forms when suspended from capillary tube tips. Furthermore, the equality of mass flow is equal to the equality of volume flow. The phase of the inlet flow (from the droplet inlet port (5) and the draft inlet port (4) is used to determine the outlet port (6) flow phase.