公开(公告)号：JP2001000186A

公开(公告)日：2001-01-09

申请号：JP17084099

申请日：1999-06-17

Applicant: TOSOH CORP

Inventor： MATSUBA TAKAO ,  YASUKAWA KIYOSHI

IPC: C12N15/09 ,  C07K14/72 ,  C12N5/10 ,  C12P21/02

Abstract: PROBLEM TO BE SOLVED: To obtain a new TSHR (Tag-TSHR) having high purity which consists of thyroid-stimulating hormone receptor(TSHR) whose N- or C-terminus is fused with a peptide and is useful for developing a medicine for treating Basedow's disease, and the like. SOLUTION: This is a new TSHR (Tag-TSHR) having high purity which consists of thyroid-stimulating hormone receptor(TSHR) and is useful in the field of fundamental studies such as the elucidation of epitope in TSHR which is recognized with antiTSHR autoantibody and the establishment of a TSHR- reacting T-lymphocyte and for developing a medicine for treating Basedow's disease and so on, wherein N- or C-terminus of the receptor(TSHR) is fused with a peptide. This Tag-TSHR is obtained by extracting mRNA from a human thyroid cell, preparing a cDNA library by the conventional method, screening the obtained library with antiTSHR antibody to give a gene coding for TSHR, linking myc peptide gene to signal peptide region at the end of the gene, incorporating the gene into an expression vector, transducing the obtained substance into CHO cell line to transform it, followed by culturing the obtained transformant.

公开(公告)号：JPH09313183A

公开(公告)日：1997-12-09

申请号：JP2366897

申请日：1997-02-06

Applicant: TOSOH CORP

Inventor： MATSUBA TAKAO ,  YASUKAWA KIYOSHI

IPC: G01N33/573 ,  C12N5/10 ,  C12N9/06 ,  C12N15/09 ,  C12R1/91

Abstract: PROBLEM TO BE SOLVED: To obtain a cell line in myeloma strain which can express human glutamic acid decarboxylase(GAD) and enables the production of a large amount of human recombinant GAD useful in specific detection and measurement of anti-GAD antibody as a very effective diagnosis for insulin-dependent diabetes or the like. SOLUTION: GAD 65 gene is cloned by amplifying cDNA library of human pancreas through the PCR technique using a primer and the cloned gene is treated with restriction enzymes and inserted into the mammalian cell expression plasmid BCMGSneo comprising cytomegarovirus promoter (CMV-P), polyadenylate attachment signal (poly A), neomycin-resistant gene (Neo) to prepare a human GAD65 expressing plasmid BCMGS-GAD. This recombinant plasmid is introduced into mouse myeloma cells with the electroporation technique whereby a novel myeloma cell line expressing recombinant human glutamic acid decarboxylase(GAD) is obtained.

公开(公告)号：JPH0658939A

公开(公告)日：1994-03-04

申请号：JP22937592

申请日：1992-08-06

Applicant: TOSOH CORP

Inventor： AZUMA TAKESHI ,  HORI HITOSHI ,  MATSUBA TAKAO ,  IIDA HIROSHI ,  IGARASHI KOJI ,  YAMADA MASAYUKI

IPC: G01N33/53 ,  A61K39/395 ,  C07K16/30 ,  C07K16/40 ,  C12N9/64 ,  C12Q1/68 ,  G01N33/574

Abstract: PURPOSE:To establish a diagnostic method using a cancer marker produced specifically in a cancerous cell, by making an anti-cathepsin E antibody or an anti-procathepsin E antibody react with a sample and by detecting a conjugated body of cathepsin E or procathepsin E in the sample and the antibody. CONSTITUTION:A diagnosis of cancer is executed by making an anti-cathepsin E antibody or an anti-procathepsin E antibody react with a sample and by detecting a conjugated body of cathepsin E or procathepsin E in the sample and the antibody. The cathepsin can be detected by making the antibody thereof react therewith and by detecting the conjugated body being a reaction product. As for the sample, a tissue slice of each sort of internal organ or a liquid constituent of an organism such as a serum or urine is used. In the case when the tissue slice is used as the sample, the tissue slice is subjected to immunological dyeing by using the anti-cathepsin E antibody and the presence or absence of revelation of the cathepsin E is observed. When the sample is the liquid constituent of the organism, the anti-cathepsin E antibody is used and the cathepsin E is detected by a measuring method such as a sandwich method or a competing method, for instance.

公开(公告)号：JPH04338345A

公开(公告)日：1992-11-25

申请号：JP13707991

申请日：1991-05-14

Applicant: TOSOH CORP

Inventor： MATSUBA TAKAO ,  KAWAHATA KOUJI

IPC: C07C17/02 ,  C07C23/02

Abstract: PURPOSE:To provide selectively the gamma-modification of 1,2,5,6,9,10- hexabromocyclododecanes excellent in heat resistance. CONSTITUTION:The objective gamma-modification of 1,2,5,6,9,10- hexabromocyclododecanes can be obtained selectively by alternately charging a reaction solvent with bromine and 1,5,9-cis, trans, trans-cyclododecatriene to carry out reaction.

公开(公告)号：JPS6451435A

公开(公告)日：1989-02-27

申请号：JP20835087

申请日：1987-08-24

Applicant: TOSOH CORP

Inventor： MATSUBA TAKAO ,  SHINTANI KOJI ,  KANESHIGE YOSUKE

IPC: C08G73/00

Abstract: PURPOSE:To obtain a moldable polyaniline excellent in electroconductivity, by eliminating the carboxyl groups from a polyaniline derivative having carboxyl groups in the benzene rings by decarboxylation. CONSTITUTION:A polyaniline derivative having carboxyl groups in the benzene rings, obtained by polymerizing an aniline derivative of the formula (wherein R1-4 are each H or COOH and at least one of them is COOH) by a chemical or electrolytic oxidation process, is dissolved in an organic solvent such as dimethylacetamide, and is formed into a film on a base by, for example, casting. The formed film is heated to 100-500 deg.C for 10min-24hr to effect decarboxylation.

公开(公告)号：JPS6451434A

公开(公告)日：1989-02-27

申请号：JP20644387

申请日：1987-08-21

Applicant: TOSOH CORP

Inventor： MATSUBA TAKAO ,  SHINTANI KOJI ,  KANESHIGE YOSUKE

IPC: C08G73/00

Abstract: PURPOSE:To obtain the title polymer which is soluble in a solvent, excellent in moldability, capable of forming an electroconductive polymer film and useful in the filed of electrical and electronic industries, by oxidizing a polymer obtained by reacting a dialkylsuccinyl succinate with phenylenediamine. CONSTITUTION:A polymer of formula II (wherein n=2-100) is obtained by dissolving a dialkylsuccinyl succinate of formula I (wherein R1 and R2 are each H or a 1-12C alkyl and, when it is H, this polymer may be in the form of a carboxylate with a basic component or a metal) and phenylenediamine at a molar ratio of 1:1 in a solvent such as ethanol in a concentration of 10-40wt.%, and reacting this solution at a temperature of 0 deg.C to the b.p. of the solvent for 30min-24hr. This polymer is oxidized with an oxidizing agent such as chloranil or p-chloronitrobenzene at a temperature of 0 deg.C to the b.p. of the solvent for 30min-24hr in a solvent such as dimethylacetamide to obtain the title polymer having a polyaniline structure of formula III as a repeating structural unit.

公开(公告)号：JP2012140331A

公开(公告)日：2012-07-26

申请号：JP2010291802

申请日：2010-12-28

Applicant: Tosoh Corp ,  東ソー株式会社

Inventor： MATSUBA TAKAO

IPC: C07K7/08 ,  C07K1/14 ,  C07K7/06 ,  C07K14/58 ,  C07K16/18 ,  C07K19/00 ,  C12N15/09 ,  G01N33/68

Abstract: PROBLEM TO BE SOLVED: To provide a tag peptide improved with its specificity and reactivity more than those of conventional ones, and also capable of being used for the uses of immunological measurements, and methods for purifying and detecting a protein by using the peptide.SOLUTION: The oligopeptide consisting of 16 amino acids of a cyclic part peptide in a type B natriuresis peptide (BNP), or the peptide consisting of at least 10 consecutive amino acids in the oligopeptide is used as the tag peptide, and the protein can be purified and detected by using the protein imparted with the tag peptide and a substance recognizing the tag peptide.

Abstract translation: 待解决的问题：提供一种改进的特异性和反应性比标准肽更好的标签肽，并且还能够用于免疫学测量的用途，以及通过使用方法来纯化和检测蛋白质的方法 肽。 解决方案：将B型钠尿肽（BNP）中的环状肽的16个氨基酸组成的寡肽或由寡肽中至少10个连续氨基酸组成的肽作为标签肽， 可以通过使用赋予标签肽的蛋白质和识别标签肽的物质来纯化和检测蛋白质。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2009240300A

公开(公告)日：2009-10-22

申请号：JP2008306713

申请日：2008-12-01

Applicant: Tosoh Corp ,  東ソー株式会社

Inventor： MATSUBA TAKAO

IPC: C12P21/08 ,  C12N15/09

CPC classification number: C07K16/00

Abstract: PROBLEM TO BE SOLVED: To provide a rabbit monoclonal antibody having high affinity, without using rabbit myeloma cells. SOLUTION: The production of a genetically engineered antibody expressing a genetically engineered rabbit monoclonal antibody is enabled by isolating antibody-producing cells from a rabbit having been immunized with an antigen, proliferating cells carrying a target antibody gene and then screening, isolating heavy chain and light chain antibody genes from the screened cells and amplifying the same, transferring the same into an expression vector and further transferring into host cells. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 待解决的问题：提供不使用兔骨髓瘤细胞的具有高亲和力的兔单克隆抗体。 解决方案：通过从已经用抗原免疫的兔子分离产生抗体的抗体产生细胞，携带靶抗体基因的增殖细胞，然后筛选，分离重组 来自筛选细胞的链和轻链抗体基因并将其扩增，转移到表达载体中并进一步转移到宿主细胞中。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2003047478A

公开(公告)日：2003-02-18

申请号：JP2001240874

申请日：2001-08-08

Applicant: Tosoh Corp ,  東ソー株式会社

Inventor： TSUCHIYA SHIGEO ,  MASUDA NORIYOSHI ,  MATSUBA TAKAO ,  ISHIGURO NORIHIKO

IPC: G01N33/569 ,  C12N15/09 ,  C12Q1/06 ,  C12Q1/68

Abstract: PROBLEM TO BE SOLVED: To provide a combination of oligonucleotides that is effective for carrying out a specific amplification of an RNA originating from a pab gene of Mycobacterium tuberculosis and achieving high-sensitivity detection and identification of the bacterium.
SOLUTION: In the gene detection of Mycobacterium tuberculosis by utilizing an RNA multiplication process, an oligonucleotide having at least ≥10 successively sequenced bases is used as a first primer, an oligonucleotide having at least ≥10 successively sequenced bases is used as a second primer and an in vitro double stranded DNA transcription process is employed for multiplying the RNA.
COPYRIGHT: (C)2003,JPO

Abstract translation: 要解决的问题：提供对进行来自结核分枝杆菌的pab基因的RNA的特异性扩增有效的寡核苷酸的组合，并实现细菌的高灵敏度检测和鉴定。 解决方案：通过利用RNA增殖方法在结核分枝杆菌的基因检测中，使用具有至少≥10个连续测序的碱基的寡核苷酸作为第一引物，将具有至少≥10个相继测序的碱基的寡核苷酸用作第二引物 引物和体外双链DNA转录过程用于倍增RNA。

公开(公告)号：JP2002345476A

公开(公告)日：2002-12-03

申请号：JP2001157413

申请日：2001-05-25

Applicant: TOSOH CORP

Inventor： MATSUBA TAKAO ,  ISHIGURO NORIHIKO

IPC: G01N33/532 ,  C12N15/09 ,  C12Q1/68

Abstract: PROBLEM TO BE SOLVED: To provide a simple method for measurement with a high sensitivity by which information indicating the approach of a labeled substance to a substance as an object of measurement can be amplified to raise an S/N ratio and the labeled substance is readily bound. SOLUTION: This method for measurement comprises bringing (a) the substance as the object of measurement into contact with a substance capable of specifically binding to substances in (b) and (c), (b) a substance capable of specifically binding to (a) and bound to the labeled nucleic acid, (c) another substance capable of specifically binding to (a) and bound to the labeled nucleic acid and (d) a sample, ligating the labeled nucleic acids of (b) and (c), amplifying a part containing at least the ligated part of the ligated nucleic acids or causing the synthesis of a nucleic acid indicating that the labeled nucleic acids are ligated, detecting the amplified nucleic acid and relating the obtained results to the presence or the amount of the present substance as the object of measurement.