公开(公告)号：KR100870243B1

公开(公告)日：2008-11-25

申请号：KR1020070051133

申请日：2007-05-25

Applicant: 고려대학교 산학협력단

Inventor： 조봉래

IPC: G01N21/91

Abstract: Two-photon dye for real-time monitoring of cells, in which the charge transport within molecule is facilitated because of having high deltaTPA by holding strong donor-acceptor pairs within the annular planar structure, the optical stability is excellent because of having the conjugated bond within the loop, the water solubility is excellent because of having the integrated ester as receptor, and the cell permeability is superior because of having a small molecular weight, is provided. And a preparing method of the two-photon dye and a real-time cell monitoring method using the same are also provided. Two-photon dye for real time monitoring of cells is represented by a formula 1. In the formula 1, X is -OCH3, and -OH or - N(CH3) 2, and Y is -COOCH3, and -CN or -COOC4H4NO2. The maximum wavelength of the two-photon excitation spectrum of the compound in the formula 1 ranges from 780nm to 940nm.

Abstract translation: 用于实时监测细胞的双光子染料，其中由于通过在环形平面结构内保持强供体 - 受体对而具有高δTPA，因此分子内的电荷传输得到促进，由于具有共轭键，所以光学稳定性是优异的 在环内，由于具有一体化的酯作为受体，所以水溶性是优异的，并且由于具有小分子量而提高了细胞的透过性。 还提供了双光子染料的制备方法和使用其的实时细胞监测方法。 在式1中，X为-OCH 3，-OH或-N（CH 3）2，Y为-COOCH 3，-CN或-COOC 4 H 4 NO 2 。 式1中化合物的双光子激发光谱的最大波长范围为780nm至940nm。

公开(公告)号：KR100805262B1

公开(公告)日：2008-02-20

申请号：KR1020070064801

申请日：2007-06-28

Applicant: 고려대학교 산학협력단

Inventor： 조봉래

IPC: C09B17/04

CPC classification number: C09B57/00 ,  C07C229/18 ,  C07C309/14 ,  C07F9/106 ,  G01N33/533

Abstract: A two-photon dye for real-time imaging of lipid raft is provided to realize high solubility in water and high fluorescence intensity under an environment inside cellular membranes without causing cytotoxicity, and to enable direct visualization and three-dimensional real-time imaging of lipid raft. A two-photon dye for real-time imaging of lipid raft is a compound represented by the following formula 1. In formula 1, R1 is a C5-C17 alkyl group; R2 is -COOH, -CH2SO3Na or the formula (a); and R3 is a C5-C17 alkyl group. The two-photon dye for real-time imaging of lipid raft has an in vivo two-photon absorptivity of at least 20 GM, and the two-photon excitation occurs under a light source with a wavelength range of 750-1000nm and fluorescence occurs at a wavelength of 400-700 nm.

Abstract translation: 提供了用于脂筏实时成像的双光子染料，以在细胞膜内的环境下实现在水中的高溶解度和高荧光强度，而不引起细胞毒性，并且使得脂质的直接可视化和三维实时成像 筏。 用于脂筏实时成像的双光子染料是由下式1表示的化合物。在式1中，R 1是C 5 -C 17烷基; R2是-COOH，-CH2SO3Na或式（a）; R3为C5-C17烷基。 用于脂筏实时成像的双光子染料具有至少20GG的体内双光子吸收率，双光子激发发生在波长范围为750-1000nm的光源下，荧光发生在 波长400-700nm。