公开(公告)号：KR1020070120361A

公开(公告)日：2007-12-24

申请号：KR1020060055026

申请日：2006-06-19

Applicant: 대한민국(농촌진흥청장)

Inventor： 한길수 ,  허정욱 ,  김시찬 ,  이용범 ,  김상철 ,  임동혁 ,  최홍기

IPC: C12M3/06 ,  C12M3/00

CPC classification number: C12M29/04 ,  C12M29/20 ,  C12M41/06 ,  C12M41/48

Abstract: A culture system for micropropagating and acclimatizing plantlets is provided to allow propagating, rooting and acclimatizing of the plantlet to be continuously done in the culture tank by culturing a portion of the plantlets in an aseptic culture tank. A culture system for micropropagating and acclimatizing plantlets comprises a culture part(100) including a culture tank(105) for culturing the plantlets, a culture tank cover(110) for keeping the culture tank aseptic, an airtight packing(120) for allowing the culture tank cover to completely seal the culture tank, an opening door equipped at one side of the culture tank, a culture solution tank(125) supplying a culture solution, a culture solution inlet(130) formed at one side of the culture tank, a culture solution outlet(140) formed at the other side of the culture tank, a first air supplying unit(150) where air required for growing the plantlets is supplied, an air outlet(160) where the air in the culture tank is discharged, a first air aseptic filter equipped at the first air supplying port, a second air aseptic filter(165) removing bacteria and fungi from the air discharged from the air outlet, a first liquid aseptic filter equipped at the culture solution inlet to supply the aseptic state culture solution, a second liquid aseptic filter for filtering the culture solution discharged from the culture solution outlet and then discharging it in the aseptic state, a far infrared rays sterilizing lamp(175) for sterilizing the culture solution to be supplied to the culture tank, and a contamination detecting sensor(170) which measures the electrical conductivity(EC), hydrogen ion concentration(pH) and the temperature of the culture solution to determine the contamination of the culture solution; an integrated controlling device(200) including a first motor, a second motor supplying air required for the plantlets cultured in the culture tank through the first air supplying unit to the culture tank, a contamination alarming device generating an alarm sound to inform the contamination of the culture solution, an intensity of illumination controlling device, and a display portion(260); a ventilation controlling device(300) directly supplying air required for the growth of the plantlets; and an illumination intensity adjusting device(400) located at an upper part of the culture tank cover to supply light required for photosynthesis.

Abstract translation: 提供了一种用于微繁殖和驯化小植株的培养系统，以便通过在无菌培养箱中培养一部分小植株使培养罐中连续进行的小植物的繁殖，生根和驯化。 一种用于微繁殖和驯化小植物的培养系统包括：培养部分（100），包括用于培养小植株的培养罐（105），用于保持无菌培养箱的培养罐盖（110），用于允许 培养罐盖，用于完全密封培养罐，设置在培养罐一侧的开门，提供培养液的培养溶液罐（125），形成在培养罐一侧的培养液入口（130） 在培养罐的另一侧形成的培养液出口（140），供给生长小植物所需的空气的第一空气供给单元（150），培养罐中的空气排出的排气口（160） 第一空气无菌过滤器，设置在第一空气供应口处;第二空气无菌过滤器，从空气出口排出的空气中除去细菌和真菌;第一液体无菌过滤器，装在培养液入口处，供应灰浆 透皮状态培养液，第二液体无菌过滤器，用于过滤从培养液出口排出的培养液，然后以无菌状态排出;远红外线灭菌灯（175），用于对培养液进行灭菌 罐和污染检测传感器（170），其测量培养溶液的电导率（EC），氢离子浓度（pH）和温度以确定培养溶液的污染; 集成控制装置（200），包括第一电动机，第二电动机，通过第一空气供给单元向培养罐供给培养槽中培养的小植物所需的空气;产生警报声音以通知污染的污染报警装置 培养溶液，照明控制装置的强度和显示部分（260）; 直接供应小植株生长所需的空气的通风控制装置（300）; 以及位于培养罐盖的上部的照明强度调节装置（400），以供应光合作用所需的光。