公开(公告)号：KR100686691B1

公开(公告)日：2007-02-26

申请号：KR1020050106465

申请日：2005-11-08

Applicant: 숙명여자대학교산학협력단

Inventor： 박종훈 ,  김기원 ,  서영주 ,  정민숙 ,  양문희 ,  육연주

IPC: C12N15/65

Abstract: A biomarker gene of beta-arrestin 2 for confirming drug addiction and dependence is provided to diagnose drug addiction and dependence in the gene level by analyzing expression of a related gene. The biomarker gene of beta-arrestin 2 for confirming drug addiction and dependence has the nucleotide sequence of SEQ ID NO:5 and is characterized by increase of its expression when a subject is addicted by a drug selected from methamphetamine, amphetamine, ecstasy and cocaine. A method for analyzing expression of beta-arrestin 2 gene comprises the steps of: extracting RNA from striatum, hippocampus, nucleus accumbens and cingulated cortex in the mesolimbic pathway of the drug addiction-induced brain, and reverse-transcribing the extracted RNA to prepare cDNA; PCR amplifying the cDNA as a template using a primer set of SEQ ID NO:1 and SEQ ID NO:2 or SEQ ID NO:3 and SEQ ID NO:4; and analyzing the expression of the amplified gene.

Abstract translation: 通过分析相关基因的表达，提供了用于确认药物成瘾和依赖性的β-抑制蛋白2的生物标志物基因以诊断药物成瘾和基因水平的依赖性。 用于确认药物成瘾和依赖性的β抑制蛋白2的生物标志物基因具有SEQ ID NO：5的核苷酸序列，并且其特征在于当受试者被选自甲基苯丙胺，苯异丙胺，摇头丸和可卡因的药物上瘾时表达增加。 用于分析β-抑制蛋白2基因表达的方法包括以下步骤：从药物成瘾诱导脑的中脑边缘途径中的纹状体，海马，伏隔核和扣带皮层提取RNA，并逆转录所提取的RNA以制备cDNA ; 使用SEQ ID NO：1和SEQ ID NO：2或SEQ ID NO：3和SEQ ID NO：4的引物组作为模板PCR扩增cDNA; 并分析扩增基因的表达。