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公开(公告)号：KR1020130141236A
公开(公告)日：2013-12-26
申请号：KR1020120064490
申请日：2012-06-15
Applicant: 한국과학기술연구원
Abstract: The present statement provides a step of proceeding tyrosinase in lignin and making the mixture react, as a method for degrading a catalyst with lignin. When using the method, tyrosinase dose not need an alternate group, lignin is effectively degraded due to stability and activity, in a more eco-friendly way then heat or oxidation treatment, and lignin is also effectively degraded from ligro-cellulosic biomass, leading to easy production of bio fuel.
Abstract translation: 本说明书提供了在木质素中进行酪氨酸酶并使混合物反应的步骤，作为用木质素降解催化剂的方法。 当使用该方法时，酪氨酸酶不需要替代组，木质素由于稳定性和活性而有效降解，以更环保的方式进行热或氧化处理，木质素也有效地从纤维素生物质中降解，导致 容易生产生物燃料。
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公开(公告)号：KR1020130088361A
公开(公告)日：2013-08-08
申请号：KR1020120009559
申请日：2012-01-31
Applicant: 에스케이이노베이션 주식회사 , 한국과학기술연구원
CPC classification number: C12P7/26 , C07C45/90 , C07C49/04 , C12Y401/01004
Abstract: PURPOSE: A method for preparing butanone from levulinic acid using a decarboxylase biocatalyst is provided to produce butanone which is a biofuel for various applications by enzymatic decarboxylation of levulinic acid under the presence of a biocatalyst. CONSTITUTION: A method for preparing butanone comprises the step of performing decarboxylization of levulinic acid under the presence of an acetoacetate decarboxylase (AADC) biocatalyst, an AADC expressing microorganism, or a mixture thereof and an electron transport mediator. The electron transport mediator is methyl viologen, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), or a mixture thereof. The AACD biocatalyst is prepared from Clostridium sp., Psudomonas sp., Chromobacterium sp., or Paenibacillus sp. [Reference numerals] (AA) Relative activity(%); (BB) Concentration of a substrate(mM)
Abstract translation: 目的：提供一种使用脱羧酶生物催化剂从乙酰丙酸制备丁酮的方法，以生产丁酮，其为生物催化剂存在下通过酶解脱乙酰丙酸的各种应用的生物燃料。 构成：制备丁酮的方法包括在乙酰乙酸脱羧酶（AADC）生物催化剂，AADC表达微生物或其混合物和电子传递介体存在下进行乙酰丙酸的脱羧的步骤。 电子转运介体是甲基紫精，2,2'-氮杂双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）或其混合物。 AACD生物催化剂由梭菌属（Psudomonas sp。），色杆菌属（Chromobacterium sp。）或Paenibacillus sp。 （AA）相对活性（％） （BB）底物浓度（mM）
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公开(公告)号：KR1020130087298A
公开(公告)日：2013-08-06
申请号：KR1020120008539
申请日：2012-01-27
Applicant: 한국과학기술연구원
CPC classification number: C12P7/40 , C07C51/42 , C07C53/126 , C12N1/20
Abstract: PURPOSE: A method for preparing hexanoic acid using in situ extractive fermentation is provided to produce and easily collect hexanoic acid of high yield using sucrose as a carbon source. CONSTITUTION: A method for preparing hexanoic acid using a microorganism comprises the steps of: inoculating hexanoic acid-producing strains to a culture medium and culturing; adding an organic solvent to the culture medium; continuously culturing the strains; and removing the organic solvents and obtaining hexanoic acid.
Abstract translation: 目的：提供使用原位萃取发酵制备己酸的方法，以蔗糖为碳源生产和容易收集高产率的己酸。 构成：使用微生物制备己酸的方法包括以下步骤：将产酸生产菌株接种到培养基中并培养; 向培养基中加入有机溶剂; 不断培养菌株; 除去有机溶剂并得到己酸。
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44.发明公开클로스트리디움 속 균주, 상기 균주를 이용한 부티르산 생산방법 및 상기 균주의 분리방법 有权CLOSTRIDIUM SP。 菌株，使用柠檬酸生产丁酸的方法。 菌株和用于分离菌丝体的方法。 应变
公开(公告)号：KR1020130077296A
公开(公告)日：2013-07-09
申请号：KR1020110145926
申请日：2011-12-29
Applicant: 한국과학기술연구원
Abstract: PURPOSE: A butyric acid producing method using a strain from Clostridium is provided to effectively produce butyric acid from galactan, a marine algae biomass, having a main carbohydrate component by producing butyric acid form galactose. CONSTITUTION: A strain from Clostridium uses galactose to produce butyric acid. A butyric acid producing method from galactose comprises treating the strain from the Clostridium on galactose and culturing to convert galactose to butyric acid. A separating method of the strain from the Clostridium comprises a step of removing a gram-negative bacteria by heat treating an anaerobic sludge, a step of inoculating the heat treated anaerobic sludge to the culture medium containing galactose and culturing, a step of selecting colonies with butyric acid resistancy by smearing the culturing solution obtained from the culturing to the culture medium containing butyric acid or the salt, and a step of separating the most butyric acid producing strain by measuring butyric acid of the selected colonies.
Abstract translation: 目的：提供使用来自梭菌的菌株的丁酸生产方法，以有效地从半乳聚糖（海藻藻类生物质）中产生具有主要碳水化合物组分的丁酸，通过产生丁酸形成半乳糖。 构成：来自梭菌的菌株使用半乳糖来生产丁酸。 来自半乳糖的丁酸生产方法包括在半乳糖上处理来自梭菌的菌株并培养以将半乳糖转化为丁酸。 来自梭菌的菌株的分离方法包括通过热处理厌氧污泥去除革兰氏阴性细菌的步骤，将热处理的厌氧污泥接种到含有半乳糖的培养基中并培养的步骤，选择具有 通过将从培养获得的培养溶液涂抹到含有丁酸或其盐的培养基中的丁酸抗性，以及通过测定所选择的菌落的丁酸来分离最多的丁酸生产菌株的步骤。
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公开(公告)号：KR101144235B1
公开(公告)日：2012-05-10
申请号：KR1020100017274
申请日：2010-02-25
Applicant: 한국과학기술연구원
CPC classification number: C12P7/08 , C12P3/00 , C12P7/065 , C12P7/16 , Y02E50/10 , Y02E50/17 , Y02E50/343
Abstract: 본 발명은 바이오에탄올 생산 과정에서 발생하는 폐발효액에 부탄올 생산 미생물을 배양하는 것을 특징으로 하는 부탄올의 제조방법에 관한 발명으로, 바이오에탄올 생산 과정에서 발생하는 폐발효액 내의 글리세롤은 혐기성 상태에서 미생물에 의해 부탄올로 전환된다. 본 발명은 바이오에탄올 생산 후 발생하는 폐발효액을 바이오연료의 원료로 사용함으로써 환경과 에너지 문제를 동시에 해결하는 효과가 있다.
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Patent Agency Ranking
公开(公告)号：KR102173211B1
公开(公告)日：2020-11-03
申请号：KR20190061921
申请日：2019-05-27
Applicant: 한국과학기술연구원
Abstract: 본명세서는자일로스와글루코스를동시에탄소원으로이용할수 있는형질전환효모균주, 이의제조방법및 이를이용한바이오연료생산방법에관하여기술한다. 상기형질전환효모균주는자일로스를탄소원으로이용하지못하는야생형효모균주를형질전환하여글루코스와자일로스를동시에전환하여고수율의바이오연료생산이가능하다. 추가적인개량을통하여고수율의바이오연료/소재생산균주로의전환이용이한균주를제공함으로써바이오연료및 바이오소재생산공정의경제성및 지속가능성을크게향상시킬수 있다.
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公开(公告)号：KR101806201B1
公开(公告)日：2017-12-07
申请号：KR1020150050321
申请日：2015-04-09
Applicant: 한국과학기술연구원
CPC classification number: C12P7/52 , C12P19/02 , C12P19/12 , C12P2203/00 , Y02E50/17 , Y02E50/343
Abstract: 본발명은목질계및 해조류혼합바이오매스당화액에관한것으로, 해조류바이오매스에목질계바이오매스를혼합하여당화액을제조함으로써목질계바이오매스유래아세트산이해조류바이오매스유래만니톨의전자수용체로작용하여발효저해물질을매우적게함유하면서도높은당 생산농도를유지할수 있다. 이에본 발명은목질계바이오매스가갖는목질계유래발효저해물질로인한발효효율저하와해조류바이오매스가갖는장시간의발효시간에도불구하고생산농도가매우낮다는문제를해결할수 있어, 상기본 발명에따른당화액을온실가스발생저감형바이오연료및 화학원료생산산업에적용하면경제적인바이오연료의생산이가능하다.
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公开(公告)号：KR1020170119264A
公开(公告)日：2017-10-26
申请号：KR1020160047227
申请日：2016-04-18
Applicant: 한국과학기술연구원
IPC: C12N15/113 , C12N9/02 , C12N15/74 , C12R1/145 , C12P7/52
CPC classification number: C12N15/113 , C12N9/0067 , C12N15/74 , C12N2310/11 , C12N2310/14 , C12P7/52 , C12R1/145
Abstract: 본명세서에는수소화효소유전자발현을억제하기위한안티센스 RNA 분자, 그를포함하는조성물, 그를암호화하는 DNA 서열을포함하는벡터, 상기안티센스 RNA 분자또는그를암호화하는 DNA 서열을포함하거나또는상기벡터로형질전환된숙주세포, 및그 숙주세포를이용한물질생산방법이개시된다. 이를이용하면수소화효소발현을억제시킬수 있고그에따라수소생산이감소하여부티르산과같이유용한물질생산을증가시키면서아세트산과같은부산물을감소시킬수 있고, 목당을효과적으로활용하여부티르산과같은유용물질을생산할수 있다.
Abstract translation: 本文所包含的编码矢量，其中，所述反义RNA分子的DNA序列或他包含编码所述组合物的DNA序列，包含他反义RNA分子，他用于抑制氢化基因表达，或用该载体转化 宿主细胞和宿主细胞。 通过使用此sikilsu抑制氢化酶表达，从而增加有用物质的生产，如丁酸，以减少氢产量可和sikilsu减少副产物如乙酸，有效地利用木糖以生产有用的物质如酸。
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