Abstract:
본 발명은 바실러스 서브틸리스의 ywbO 유전자로부터 발현되는 설퍼 옥시도리덕타아제와 특이적으로 결합하는 형광 화합물을 포함하는 바실러스 서브틸리스 검출 키트 및 이를 이용한 바실러스 서브틸리스 검출 방법에 관한 것이다. 일 구체예에 따르면, 본 발명의 방법 및 키트는 3',6'-비스(아세틸옥시)-5-(클로로메틸)-스피로(이소벤조퓨란-1(3H),9'-(9H)잔텐)-3-온에 특이적으로 결합하는 바실러스 서브틸리스의 ywbO 유전자로부터 발현되는 설퍼 옥시도리덕타아제를 검출하여 바실러스 서브틸리스와 엔테로코쿠스 패칼리스를 구별하므로, 쉽고 빠르며 정확한 동정 결과를 제공할 수 있다.
Abstract:
본발명은형광화합물을이용하여헤파티티스 C 바이러스가감염된간세포와정상세포를구별하는방법에관한것이다. 일구체예에따르면, 본발명의방법은 2-(6-히드록시-3-옥소-3H-잔텐-9-일)-5-((2-(비닐술포닐)에틸)카바모일)벤조산및 2-(6-히드록시-3-옥소-3H-잔텐-9-일)-4-((2-(비닐술포닐)에틸)카바모일)벤조산에결합하는단백질패턴을비교하여헤파티티스 C 바이러스가감염된간세포와정상세포를구별하므로, 쉽고빠르며정확한동정결과를제공할수 있다.
Abstract:
The present invention relates to a detection kit for Enterobacter cloacae including fluorophores which are specifically bonded to 6-phosphofructokinase expressed from pfkB gene of the Enterobacter cloacae and a method for detecting Enterobacter cloacae using the same. According to one specific example, the method and kit of the present invention provide easy, rapid and accurate identification by detecting 6-phosphofructokinase expressed from the pfkB gene of the Enterobacter cloacae which specifically bonds to 3',6'-bis(acetyloxy)-5-(chloromethyl)-spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one and distinguishing Enterobactercloacae from Enterococcusfaecalis.
Abstract:
The present invention relates to a method for distinguishing Enterobacter cloacae and Escherichia coli using fluorescent compounds. According to a specific example, the method easily, quickly, and accurately provides identification results by comparing the pattern of proteins which are conjugated to 3’,6’-bis(acetyloxy)-5-(chloromethyl)-spiro(isobenzofuran-1(3H),9’-(9H)xanthene)-3-one and distinguishing Enterobacter cloacae and Escherichia coli.
Abstract:
The present invention relates to a method for distinguishing various kinds of Escherichia coli species using fluorescent compounds. According to a specific example, the method easily, quickly, and accurately provides identification results by comparing the pattern of proteins which are conjugated to 3’,6’-bis(acetyloxy)-5-(chloromethyl)-spiro(isobenzofuran-1(3H),9’-(9H)xanthene)-3-one and distinguishing various Escherichia species.