Abstract:
PURPOSE: A method for producing antibiotics marker-free/herbicide complex resistant creeping bentgrass plant is provided to improve weed herbicidal effect and to enhance creeping bentgrass productivity. CONSTITUTION: A method for producing antibiotics marker-free/herbicide complex resistant creeping bentgrass plant comprises: a step of transforming Agrobacterium with a plant expression vector containing herbicide resistant bar gene and CP4-EPSPS(P4 5-enolpyruvylshikimate-3- phosphate synthase) gene without an antibiotic marker gene; a step of co-culturing the transformed Agrobacteriuam and creeping bentgrass embryogenic callus; a step of selecting new shoots transformed from the embryogenic callus and growing; and a step of testing herbicide resistance using glufosinate ammonium and glyphosate. The plant expression vector is p35S-EPSPS-B vector.
Abstract:
본 발명은 주형물질을 이용하여 입자의 형태가 균일하며 그 크기분포가 좁은 분포도를 가지며 평균적인 크기 대부분 200 nm 이하의 구리를 함유한 혼합금속산화물의 제조방법, 그로부터 제조된 혼합금속산화물 촉매, 및 상기 혼합금속산화물 촉매를 이용하여 포화계 지방족 알코올을 효율적으로 제조하는 방법에 관한 것이다. 구리, 혼합금속산화물, 균일형태, 주형물질, 수소화 반응, 나노입자
Abstract:
PURPOSE: A preparation method of ceramic gel compact is provided, which controls gelation-inducing time easily without lowering the physical properties of gel compact by adding peroxide-based polymerization initiator and gelation-inducing time increaser to ceramic dispersing agent for gel casting. CONSTITUTION: The preparation method comprises the steps of: dispersing at least one ceramic powder selected from Al2O3, ZrO2, ZnO, Si3N4 and SiC, into a solution of at least one organic radical monomer selected from acryl amide, methylacryl amide and hydroxymethyl acryl amide; adding one or more of peroxide-based polymerization initiator selected from ammonium persulfate, sodium persulfate, magnesium persulfate and hydrogen peroxide, and one or more of gelation-inducing time increaser selected from nitroxyl radicals, aliphatic alcohols and aqueous azos; degassing and pouring into a mold; forming a gel compact. The nitroxyl radical-based gelation-inducing time increaser, such as 2,2,6,6,-tetramethyl piperidine-1-oxyl(TEMPO), 4-hydroxy-2,2,6,6,-tetramethyl piperidine-1-oxyl(HTEMPO), is used in a range of 5-500 ppm, based on the total amount of dispersed solution. The aliphatic alcohol-based gelation-inducing time increaser, such as methyl alcohol, ethyl alcohol, butyl alcohol, etc, is used in a range of 5-20%, based on the total amount of dispersed solution. The aqueous azo-based gelation-inducing time increaser, such as azobis(2-amidinopropane) hydrochlorate, is used within the range of 1:9-9:1(in a weight ratio), based on the peroxide-based polymerization initiator.
Abstract:
PURPOSE: A method for manufacturing a diamond tool is provided to improve cutting ability and durability by additionally reforming the surface of diamond grit and wet-coating the reformed surface of the diamond grit with nano metal powder. CONSTITUTION: A method for manufacturing a diamond tool comprises the steps of: heat-treating diamond grit, soaking the diamond grit in an acid or an alkaline solution, and reforming the surface of the diamond grit; wet-coating the reformed surface of the diamond grit with nano metal powder; applying transition metal paste to a shank base metal and forming a metal adhesive layer; and scattering the diamond grit coated with nano metal powder on the metal adhesive layer.