두 가지 제초제에 대하여 저항성을 가지는 항생제 마커프리 형질전환 크리핑 벤트그라스 식물체

    公开(公告)号:KR1020110124606A

    公开(公告)日:2011-11-17

    申请号:KR1020100044094

    申请日:2010-05-11

    CPC classification number: C12N15/8274 A01N57/20 C12N15/8205 C12N15/8223

    Abstract: PURPOSE: A method for producing antibiotics marker-free/herbicide complex resistant creeping bentgrass plant is provided to improve weed herbicidal effect and to enhance creeping bentgrass productivity. CONSTITUTION: A method for producing antibiotics marker-free/herbicide complex resistant creeping bentgrass plant comprises: a step of transforming Agrobacterium with a plant expression vector containing herbicide resistant bar gene and CP4-EPSPS(P4 5-enolpyruvylshikimate-3- phosphate synthase) gene without an antibiotic marker gene; a step of co-culturing the transformed Agrobacteriuam and creeping bentgrass embryogenic callus; a step of selecting new shoots transformed from the embryogenic callus and growing; and a step of testing herbicide resistance using glufosinate ammonium and glyphosate. The plant expression vector is p35S-EPSPS-B vector.

    Abstract translation: 目的:提供抗生素无标记/除草剂复合抗性爬行弯筋草植物的生产方法,以提高杂草除草效果,提高草坪草的生长。 构成:生产抗生素无标记/除草剂复合抗性爬行弯曲草植物的方法包括:用含有除草剂抗性bar基因和CP4-EPSPS(P4-5-烯醇丙酮酸莽草酸-3-磷酸合成酶)基因的植物表达载体转化土壤杆菌的步骤 没有抗生素标记基因; 共同培养转化的土壤杆菌和爬行的弯曲草胚发生愈伤组织的步骤; 选择从胚发生的愈伤组织转化并生长的新芽的步骤; 以及使用草铵膦和草甘膦测试除草剂抗性的步骤。 植物表达载体为p35S-EPSPS-B载体。

    세라믹 겔 성형체의 제조방법
    43.
    发明授权
    세라믹 겔 성형체의 제조방법 失效
    세라믹겔성형체의제조방법

    公开(公告)号:KR100411175B1

    公开(公告)日:2003-12-18

    申请号:KR1020000057408

    申请日:2000-09-29

    Abstract: PURPOSE: A preparation method of ceramic gel compact is provided, which controls gelation-inducing time easily without lowering the physical properties of gel compact by adding peroxide-based polymerization initiator and gelation-inducing time increaser to ceramic dispersing agent for gel casting. CONSTITUTION: The preparation method comprises the steps of: dispersing at least one ceramic powder selected from Al2O3, ZrO2, ZnO, Si3N4 and SiC, into a solution of at least one organic radical monomer selected from acryl amide, methylacryl amide and hydroxymethyl acryl amide; adding one or more of peroxide-based polymerization initiator selected from ammonium persulfate, sodium persulfate, magnesium persulfate and hydrogen peroxide, and one or more of gelation-inducing time increaser selected from nitroxyl radicals, aliphatic alcohols and aqueous azos; degassing and pouring into a mold; forming a gel compact. The nitroxyl radical-based gelation-inducing time increaser, such as 2,2,6,6,-tetramethyl piperidine-1-oxyl(TEMPO), 4-hydroxy-2,2,6,6,-tetramethyl piperidine-1-oxyl(HTEMPO), is used in a range of 5-500 ppm, based on the total amount of dispersed solution. The aliphatic alcohol-based gelation-inducing time increaser, such as methyl alcohol, ethyl alcohol, butyl alcohol, etc, is used in a range of 5-20%, based on the total amount of dispersed solution. The aqueous azo-based gelation-inducing time increaser, such as azobis(2-amidinopropane) hydrochlorate, is used within the range of 1:9-9:1(in a weight ratio), based on the peroxide-based polymerization initiator.

    Abstract translation: 目的:提供一种陶瓷凝胶致密物的制备方法,通过向陶瓷分散剂中加入过氧化物类聚合引发剂和凝胶诱导时间增加剂,可以容易地控制凝胶诱导时间而不降低凝胶致密物的物理性能。 组成:制备方法包括以下步骤:将选自Al2O3,ZrO2,ZnO,Si3N4和SiC的至少一种陶瓷粉分散到选自丙烯酰胺,甲基丙烯酰胺和羟甲基丙烯酰胺中的至少一种有机基团单体的溶液中; 加入一种或多种选自过硫酸铵,过硫酸钠,过硫酸镁和过氧化氢的过氧化物基聚合引发剂以及一种或多种选自硝酰基自由基,脂肪醇和含水偶氮基的胶凝诱导时间增加剂; 脱气并倒入模具中; 形成凝胶压块。 基于硝酰自由基的凝胶化诱导时间增加剂,例如2,2,6,6-四甲基哌啶-1-氧基(TEMPO),4-羟基-2,2,6,6-四甲基哌啶-1-氧基 氧基(HTEMPO)以基于分散溶液总量的5-500ppm的范围使用。 基于分散溶液的总量,使用基于脂族醇的凝胶诱导时间增加剂,例如甲醇,乙醇,丁醇等,其范围为5-20%。 以过氧化物为基础的聚合引发剂,基于水性偶氮的凝胶化诱导时间增加剂如偶氮二(2-脒基丙烷)盐酸盐的用量范围为1:9-9:1(重量比)。

    나노분말이 습식 코팅된 지립을 이용한 내구성이 증진된다이아몬드 공구의 제조방법
    44.
    发明公开
    나노분말이 습식 코팅된 지립을 이용한 내구성이 증진된다이아몬드 공구의 제조방법 失效
    通过使用用NANO金属粉末涂覆的金刚砂制造具有改善的耐久性的金刚石工具的方法

    公开(公告)号:KR1020030080294A

    公开(公告)日:2003-10-17

    申请号:KR1020020018851

    申请日:2002-04-08

    Abstract: PURPOSE: A method for manufacturing a diamond tool is provided to improve cutting ability and durability by additionally reforming the surface of diamond grit and wet-coating the reformed surface of the diamond grit with nano metal powder. CONSTITUTION: A method for manufacturing a diamond tool comprises the steps of: heat-treating diamond grit, soaking the diamond grit in an acid or an alkaline solution, and reforming the surface of the diamond grit; wet-coating the reformed surface of the diamond grit with nano metal powder; applying transition metal paste to a shank base metal and forming a metal adhesive layer; and scattering the diamond grit coated with nano metal powder on the metal adhesive layer.

    Abstract translation: 目的:提供金刚石工具的制造方法,通过对金刚石砂粒的表面进行额外重整,并用纳米金属粉末湿式涂覆金刚石砂粒的重整表面来提高切割能力和耐久性。 构成:用于制造金刚石工具的方法包括以下步骤:对金刚石砂粒进行热处理,将金刚石砂粒浸入酸或碱性溶液中,并重整金刚石砂粒的表面; 用纳米金属粉末对金刚石砂粒的重整表面进行湿法涂覆; 将过渡金属膏施加到柄基体金属并形成金属粘合剂层; 并将涂覆有纳米金属粉末的金刚石砂粒散布在金属粘合剂层上。

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