公开(公告)号：US20190051506A1

公开(公告)日：2019-02-14

申请号：US16069506

申请日：2017-01-10

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba

IPC: H01J49/38 ,  H01J49/42 ,  H01J49/10

CPC classification number: H01J49/38 ,  H01J49/102 ,  H01J49/422 ,  H01J49/4235 ,  H01J49/426

Abstract: Improvements to a side-on Penning trap include methods to stabilize ions in the trap. The ions are stabilized by injecting ions in the focusing region of the non-uniform DC fields produced by the pad electrodes of the trap. Ions are injected along an injection axis shifted from the central axis of a gap between a positively biased electrode pad and negatively biased electrode pad of the trap. Improvements also include methods to compensate for the Lorentz force that is produced when ions are injected into a side-on Penning trap. Electrodes of an ion injection device are DC biased so that the electrodes produce an electric field along the axis of the device that compensates for the Lorentz force. Finally, methods are provided to increase the m/z range of ions injected into a side-on Penning trap by pre-trapping ions just before injection of the ions into the trap.

公开(公告)号：US10014166B2

公开(公告)日：2018-07-03

申请号：US14894359

申请日：2014-05-29

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba

IPC: H01J49/00 ,  H01J49/06

CPC classification number: H01J49/0054 ,  H01J49/0045 ,  H01J49/0072 ,  H01J49/063

Abstract: A method and apparatus for conducting ion to charged species reactions, more particularly reactions wherein the charged species is an electron, such as ECD. The apparatus comprises first and second pathways which are orthogonal to one another. The first pathway through which ions are introduced comprises multiple multipoles with a gap situated there between. The second pathway introduces the charged species through the gap orthogonally to the first pathway. In this way, a cross-type reaction device allows ion-charged species interactions to occur.

公开(公告)号：US20180095092A1

公开(公告)日：2018-04-05

申请号：US15573594

申请日：2016-05-10

Applicant: DH Technologies Development PTE Ltd.

Inventor： Takashi Baba

IPC: G01N33/68 ,  H01J49/00

CPC classification number: G01N33/6848 ,  G01N2560/00 ,  H01J49/0054

Abstract: Systems and methods described herein can provide for “top down” mass spectrometric analysis of proteins or peptides in a sample using ExD, in some aspects via direct infusion of the sample to the ion source without on-line LC separation, while deconvoluting the ambiguity in the ExD spectra generated by impure samples. For example, methods and systems in accordance with various aspects of the present teachings can utilize patterns in charge-reduced species following ExD to correlate the ExD fragments with their precursor ions in order to more confidently identify the precursor ion from which the detected product ions originated.

公开(公告)号：US20160322208A1

公开(公告)日：2016-11-03

申请号：US15105130

申请日：2014-11-18

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba

IPC: H01J49/00 ,  H01J49/42

CPC classification number: H01J49/0095 ,  H01J49/0072 ,  H01J49/062 ,  H01J49/4225 ,  H01J49/4295

Abstract: An ion reaction device is provided having an ion injection inlet for receiving a plurality of ions and an ion ejection outlet through which ions can exit the device. The reaction device includes a plurality of non-linear rods disposed relative to one another so as to provide an axial region configured to receive a plurality of anions and cations via said ion injection inlet, and a plurality of trapping regions in communication with the axial region in which said anions and cations can be confined. A DC voltage source is adapted to apply a DC voltage across at least two of said rods so as to generate an electric field within at least a portion of said axial region for spatially separating the received anions and cations and guiding the anions into one of said trapping regions and the cations into another one of said trapping regions.

Abstract translation: 提供了一种离子反应装置，其具有用于接收多个离子的离子注入口和离子可以离开装置的离子喷射出口。 反应装置包括相对于彼此设置的多个非线性杆，以提供构造成经由所述离子注入口接收多个阴离子和阳离子的轴向区域，以及与轴向区域连通的多个捕获区域 其中阴离子和阳离子可以被限制。 直流电压源适于跨所述杆中的至少两个施加直流电压，以便在所述轴向区域的至少一部分内产生电场，用于空间分离所接收的阴离子和阳离子，并将阴离子引导到所述 捕获区域和阳离子到另一个所述捕获区域。

公开(公告)号：US11948788B2

公开(公告)日：2024-04-02

申请号：US17310989

申请日：2020-06-10

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Robert E. Haufler ,  William M. Loyd ,  Takashi Baba

IPC: H01J49/40 ,  H01J49/00

CPC classification number: H01J49/40 ,  H01J49/0031 ,  H01J49/0036

Abstract: A calibration apparatus for a mass analyzer includes an ion source device and a dual-purpose electron beam generating unit. The ion source device ionizes an analyte of a sample, producing analyte ions. The dual-purpose electron beam generating unit is positioned between the ion source device and the mass analyzer. In a first mode, the dual-purpose electron beam generating unit is used to create fragments of analyte ions of unknown mass-to-charge ratio. In a second mode, the dual-purpose electron beam generating unit is used to create ions of calibration compounds of known mass-to-charge ratio. All ions are subsequently transferred to the mass analyzer.

公开(公告)号：US11908672B2

公开(公告)日：2024-02-20

申请号：US17255597

申请日：2019-08-15

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba ,  Pavel Ryumin ,  Igor Chemushevich

IPC: H01J49/00 ,  H01J49/06 ,  H01J49/16 ,  H01J49/42

CPC classification number: H01J49/0077 ,  H01J49/0031 ,  H01J49/063 ,  H01J49/165 ,  H01J49/4215

Abstract: An ion source ionizes a compound, producing precursor ions with different m/z values. A reagent source supplies charge reducing reagent. An ion guide is positioned between a mass filter and both the ion source and the reagent source. The ion guide applies an AC voltage and DC voltage to its electrodes that creates a pseudopotential to trap the precursor ions in the ion guide below a threshold m/z. This AC voltage, in turn, causes the trapped precursor ions to be charge reduced by the reagent so that m/z values of the trapped precursor ions increase to a single m/z value above the threshold m/z. The ion guide applies the DC voltage to its electrodes relative to a DC voltage applied to electrodes of the mass filter that causes the precursor ions with m/z values increased to the single m/z value to be continuously transmitted to the mass filter.

公开(公告)号：US20230251231A1

公开(公告)日：2023-08-10

申请号：US18002533

申请日：2021-07-06

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Suya Liu ,  Takashi Baba

IPC: G01N30/72 ,  G01N30/96 ,  G01N30/88 ,  G01N33/68

CPC classification number: G01N30/72 ,  G01N30/88 ,  G01N30/96 ,  G01N33/6848 ,  G01N2030/022

Abstract: A separation time of an isomer of one or more isomers of a sialylated glycopeptide of a sample is calculated from a peak of a precursor XIC. Product ion intensities of the first group are summed at the separation time producing a first sum and product ion intensities of the second group are summed at the separation time producing a second sum using XICs of the first and second groups. A ratio of the first sum to the second sum is calculated. The ratio at the separation time is compared to predetermined ratio ranges that each corresponds to a combination of a selection from a set of the first linkage and the second linkage taken one or more times. One or more linkages of the sialic acid to the glycan of the isomer are identified from a combination found to match the ratio in the comparison.

公开(公告)号：US11430645B2

公开(公告)日：2022-08-30

申请号：US17284336

申请日：2019-10-08

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba ,  Pavel Ryumin

IPC: H01J49/00 ,  H01J49/02

Abstract: In one aspect, an electron-ion reaction module, e.g., an electron capture dissociation module, for use in a mass spectrometer is disclosed, which comprises a chamber, an electron source for generating electrons and introducing the electrons into the chamber, a gate electrode positioned relative to the electron source and the chamber, and a DC voltage source operatively coupled to the gate electrode for applying control voltages to the gate electrode. The electron-ion interaction module can further include a controller operably coupled to the DC voltage source and configured for adjusting the DC voltage applied to the gate electrode to adjust flow of electrons into the chamber.

公开(公告)号：US11335547B2

公开(公告)日：2022-05-17

申请号：US17041324

申请日：2019-04-09

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba ,  Pavel Ryumin ,  William M. Loyd

IPC: H01J49/40 ,  G16B40/10 ,  G01N33/68 ,  G01N33/577 ,  H01J49/00 ,  G16B30/10

Abstract: A separation device separates an unknown intact mAb or reduced mAb subunits of a known mAb class from a sample. An ion source device ionizes the mAb. A mass spectrometer fragments the ionized mAb using an ECD device and mass analyzes resulting product ions using a mass analyzer, producing one or more product ion spectra. Theoretical product ion peaks are calculated for one or more constant portions of the mAb class. The theoretical product ion peaks are removed from the one or more product ion spectra, producing one or more difference product ion spectra. De novo sequencing is applied to the one or more difference product ion spectra, producing one or more candidate sequences for one or more variable portions of the mAb. A genome database is searched for matches to the one or more candidate sequences, producing one or more matched sequences for the one or more variable portions.

公开(公告)号：US20220093383A1

公开(公告)日：2022-03-24

申请号：US17310989

申请日：2020-06-10

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Robert E. Haufler ,  William M. Loyd ,  Takashi Baba

IPC: H01J49/40 ,  H01J49/00

Abstract: A calibration apparatus for a mass analyzer includes an ion source device and a dual-purpose electron beam generating unit. The ion source device ionizes an analyte of a sample, producing analyte ions. The dual-purpose electron beam generating unit is positioned between the ion source device and the mass analyzer. In a first mode, the dual-purpose electron beam generating unit is used to create fragments of analyte ions of unknown mass-to-charge ratio. In a second mode, the dual-purpose electron beam generating unit is used to create ions of calibration compounds of known mass-to-charge ratio. All ions are subsequently transferred to the mass analyzer.