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公开(公告)号：US20210082678A1
公开(公告)日：2021-03-18
申请号：US17041324
申请日：2019-04-09
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： Takashi Baba , Pavel Ryumin , William M. Loyd
Abstract: A separation device separates an unknown intact mAb or reduced mAb subunits of a known mAb class from a sample. M An ion source device ionizes the mAb. A mass spectrometer fragments the ionized mAb using an ECD device and mass analyzes resulting product ions using a mass analyzer, producing one or more product ion spectra. Theoretical product ion peaks are calculated for one or more constant portions of the mAb class. The theoretical product ion peaks are removed from the one or more product ion spectra, producing one or more difference product ion spectra. De novo sequencing is applied to the one or more difference product ion spectra, producing one or more candidate sequences for one or more variable portions of the mAb. A genome database is searched for matches to the one or more candidate sequences, producing one or more matched sequences for the one or more variable portions.
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公开(公告)号：US12188944B2
公开(公告)日：2025-01-07
申请号：US17440130
申请日：2020-05-13
Applicant: DH Technologies Development PTE. LTD.
Inventor： Takashi Baba , Pavel Ryumin
Abstract: Method and devices for performing top down analysis of an antibody are described which involve utilizing an ion source to generate a plurality of ions from a sample containing at least one intact antibody. Further, transmitting said plurality of ions through a quadrupole rod set while applying an RF signal thereto and in the absence of a resolving DC voltage so as to preferentially transmit precursor ions having an m/z value greater than a low mass cutoff of about 1500 m/z from the quadrupole rod set to an ECD cell. The method and device can also performing an ECD reaction in the ECD cell on said precursor ions and also detect reaction products from the ECD reaction.
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公开(公告)号：US11551919B2
公开(公告)日：2023-01-10
申请号：US17284321
申请日：2019-10-08
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： Takashi Baba , Pavel Ryumin
Abstract: An electron-ion interaction module for use in a mass spectrometer, having a plurality of rod sets arranged relative to one another such that said rod sets share a common longitudinal axis and each of said rod sets is longitudinally separated from an adjacent rod set by a gap, each of said rod sets comprising a plurality of rods arranged around said common longitudinal axis. The module further includes at least one magnet disposed around said rod sets so as to at least partially surround one or more of said plurality of rod sets and configured to generate a static magnetic field along said longitudinal axis. The rod sets are configured to receive electrons from an electron source and ions from an ion source within an interaction volume defined by the rods. One or more RF voltage sources coupled to the plurality of rod sets applies voltages to the rods.
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公开(公告)号：US11908672B2
公开(公告)日：2024-02-20
申请号：US17255597
申请日：2019-08-15
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： Takashi Baba , Pavel Ryumin , Igor Chemushevich
CPC classification number: H01J49/0077 , H01J49/0031 , H01J49/063 , H01J49/165 , H01J49/4215
Abstract: An ion source ionizes a compound, producing precursor ions with different m/z values. A reagent source supplies charge reducing reagent. An ion guide is positioned between a mass filter and both the ion source and the reagent source. The ion guide applies an AC voltage and DC voltage to its electrodes that creates a pseudopotential to trap the precursor ions in the ion guide below a threshold m/z. This AC voltage, in turn, causes the trapped precursor ions to be charge reduced by the reagent so that m/z values of the trapped precursor ions increase to a single m/z value above the threshold m/z. The ion guide applies the DC voltage to its electrodes relative to a DC voltage applied to electrodes of the mass filter that causes the precursor ions with m/z values increased to the single m/z value to be continuously transmitted to the mass filter.
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公开(公告)号：US11430645B2
公开(公告)日：2022-08-30
申请号：US17284336
申请日：2019-10-08
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： Takashi Baba , Pavel Ryumin
Abstract: In one aspect, an electron-ion reaction module, e.g., an electron capture dissociation module, for use in a mass spectrometer is disclosed, which comprises a chamber, an electron source for generating electrons and introducing the electrons into the chamber, a gate electrode positioned relative to the electron source and the chamber, and a DC voltage source operatively coupled to the gate electrode for applying control voltages to the gate electrode. The electron-ion interaction module can further include a controller operably coupled to the DC voltage source and configured for adjusting the DC voltage applied to the gate electrode to adjust flow of electrons into the chamber.
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Patent Agency Ranking
公开(公告)号：US11335547B2
公开(公告)日：2022-05-17
申请号：US17041324
申请日：2019-04-09
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： Takashi Baba , Pavel Ryumin , William M. Loyd
Abstract: A separation device separates an unknown intact mAb or reduced mAb subunits of a known mAb class from a sample. An ion source device ionizes the mAb. A mass spectrometer fragments the ionized mAb using an ECD device and mass analyzes resulting product ions using a mass analyzer, producing one or more product ion spectra. Theoretical product ion peaks are calculated for one or more constant portions of the mAb class. The theoretical product ion peaks are removed from the one or more product ion spectra, producing one or more difference product ion spectra. De novo sequencing is applied to the one or more difference product ion spectra, producing one or more candidate sequences for one or more variable portions of the mAb. A genome database is searched for matches to the one or more candidate sequences, producing one or more matched sequences for the one or more variable portions.
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公开(公告)号：US11251029B2
公开(公告)日：2022-02-15
申请号：US17255607
申请日：2019-08-15
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： Takashi Baba , Pavel Ryumin , William M. Loyd
Abstract: A dissociation device fragments a precursor ion, producing at least two different product ions with overlapping m/z values in the dissociation device. The dissociation device applies an AC voltage and a DC voltage creating a pseudopotential that traps ions below a threshold m/z including the at least two product ions. The dissociation device receives a charge reducing reagent that causes the trapped at least two product ions to be charge reduced until their m/z values increase above the threshold m/z set by the AC voltage. The increase in the m/z values of the at least two product ions decreases their overlap. The at least two product ions with increased m/z values are transmitted to another device for subsequent mass analysis by applying the DC voltage to the dissociation device relative to a DC voltage applied to the other device.
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公开(公告)号：US20240242958A1
公开(公告)日：2024-07-18
申请号：US18559108
申请日：2022-05-03
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： Igor Chernushevich , Pavel Ryumin , Takashi Baba
CPC classification number: H01J49/4225 , H01J49/38 , H01J49/4245
Abstract: During an accumulation time period of each time cycle of an ion guide and before a ramped AC voltage is applied to at least one set of axial rods to eject ions according to m/z value, a number of steps are performed. Ions are received from outside of the ion guide through an entrance aperture and into a first cell. A low DC voltage is applied to a barrier electrode to receive ions from the first cell into a second cell. And, a high DC voltage is applied to an exit electrode to prevent ions from exiting the ion guide. During a cooling time period before the AC time period, a high DC voltage is applied to the barrier electrode to trap and cool ions in the second cell and to continue to receive ions into the first cell without being affected by the ramped AC voltage.
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公开(公告)号：US11848181B2
公开(公告)日：2023-12-19
申请号：US17423934
申请日：2020-01-31
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： Pavel Ryumin , Takashi Baba , Nic G. Bloomfield
CPC classification number: H01J49/0027 , H01J49/025
Abstract: Intensity measurements made by electron multiplier and image-charge detectors are proportional to charge state. These intensities are used to separate detected ions into different data sets and create mass spectra from the different data sets. Ion measurements are separated by charge state using (i) a single electron multiplier detector, (ii) a single image-charge detector, or (iii) multiple electron multiplier ADC detectors. Using (i), the intensity of a peak calculated from each measured pulse is compared to predetermined intensity ranges and each peak is stored in a corresponding data set. Using (ii), each measured transient time-domain signal is converted to frequency-domain peaks, the intensity of each frequency-domain peak is compared to predetermined intensity ranges, and each peak is stored in a corresponding data set. Using (iii), each detector is adapted to measure a predetermined intensity range and store calculated peaks from the measured pulses in corresponding data sets.
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公开(公告)号：US11728148B2
公开(公告)日：2023-08-15
申请号：US17650836
申请日：2022-02-11
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： Takashi Baba , Pavel Ryumin , William M. Loyd
IPC: H01J49/00
CPC classification number: H01J49/0031 , H01J49/0036 , H01J49/0054 , H01J49/0059 , H01J49/0072
Abstract: A dissociation device fragments a precursor ion, producing at least two different product ions with overlapping m/z values in the dissociation device. The dissociation device applies an AC voltage and a DC voltage creating a pseudopotential that traps ions below a threshold m/z including the at least two product ions. The dissociation device receives a charge reducing reagent that causes the trapped at least two product ions to be charge reduced until their m/z values increase above the threshold m/z set by the AC voltage. The increase in the m/z values of the at least two product ions decreases their overlap. The at least two product ions with increased m/z values are transmitted to another device for subsequent mass analysis by applying the DC voltage to the dissociation device relative to a DC voltage applied to the other device.
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