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    HIGH SENSITIVITY DETERMINATION OF THE CONCENTRATION OF ANALYTE MOLECULES OR PARTICLES IN A FLUID SAMPLE
    41.
    发明申请
    HIGH SENSITIVITY DETERMINATION OF THE CONCENTRATION OF ANALYTE MOLECULES OR PARTICLES IN A FLUID SAMPLE 审中-公开
    分析物分子或颗粒在流体样品中的浓度的高灵敏度测定

    公开(公告)号：US20100075862A1

    公开(公告)日：2010-03-25

    申请号：US12236484

    申请日：2008-09-23

    Applicant: David C. Duffy ,  David M. Rissin ,  Linan Song ,  David R. Walt

    Inventor： David C. Duffy ,  David M. Rissin ,  Linan Song ,  David R. Walt

    IPC: C40B30/04 ,  C40B40/00

    CPC classification number: G01N33/54366

    Abstract: The present invention relates to methods, systems, and kits for detecting, quantifying and/or analyzing a fluid sample comprising molecules or particles at low concentration. In certain embodiments, the methods for detection and/or quantifying analyte molecules in a sample comprise capturing a plurality of analyte molecules on a substrate (e.g., an array comprising a plurality of reaction vessels). The substrate may then be exposed to additional reaction components such as at least one binding ligand. The substrate may additionally be exposed to a precursor labeling agent molecule, wherein the precursor labeling agent molecule, in some cases, is converted to a labeling agent molecule, which may be detected, either directly or indirectly, which determination may be related to the presence of and/or may be employed to quantify the analyte molecules. Although the various aspects of the present invention may use a number of different assay formats, in one embodiment, the assays are conducted in a plurality of reaction vessels defined, at least in part, by the distal ends of fiber optic strands.

    Abstract translation: 本发明涉及用于检测，定量和/或分析低浓度分子或颗粒的流体样品的方法，系统和试剂盒。 在某些实施方案中，用于检测和/或定量样品中的分析物分子的方法包括在基底上捕获多个分析物分子（例如，包括多个反应容器的阵列）。 然后将底物暴露于另外的反应组分，例如至少一种结合配体。 底物可以另外暴露于前体标记试剂分子，其中在某些情况下，前体标记试剂分子被转化成可直接或间接检测的标记试剂分子，该测定可能与存在 和/或可以用于定量分析物分子。 尽管本发明的各个方面可以使用多种不同的测定形式，但是在一个实施方案中，测定是在多个反应容器中进行的，所述多个反应容器至少部分由纤维束的远端限定。

    Target analyte sensors utilizing Microspheres
    43.
    发明授权
    Target analyte sensors utilizing Microspheres 有权
    使用微球的目标分析物传感器

    公开(公告)号：US06327410B1

    公开(公告)日：2001-12-04

    申请号：US09151877

    申请日：1998-09-11

    Applicant: David R. Walt ,  Karri L. Michael

    Inventor： David R. Walt ,  Karri L. Michael

    IPC: G02B626

    CPC classification number: G01N33/54346 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1456 ,  G01N15/1459 ,  G01N15/1468 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7703 ,  G01N33/543 ,  G01N2015/1438 ,  G01N2015/1465 ,  G01N2035/0097 ,  Y10S359/90 ,  Y10S435/808

    Abstract: A microsphere-based analytic chemistry system and method for making the same is disclosed in which microspheres or particles carrying bioactive agents may be combined randomly or in ordered fashion and dispersed on a substrate to form an array while maintaining the ability to identify the location of bioactive agents and particles within the array using an optically interrogatable, optical signature encoding scheme. A wide variety of modified substrates may be employed which provide either discrete or non-discrete sites for accommodating the microspheres in either random or patterned distributions. The substrates may be constructed from a variety of materials to form either two-dimensional or three-dimensional configurations. In a preferred embodiment, a modified fiber optic bundle or array is employed as a substrate to produce a high density array. The disclosed system and method have utility for detecting target analytes and screening large libraries of bioactive agents.

    Abstract translation: 公开了一种基于微球的分析化学体系及其制备方法，其中携带生物活性剂的微球或颗粒可以随机或以有序的方式组合并分散在基质上以形成阵列，同时保持识别生物活性物质的位置的能力 阵列内的代理和粒子，使用光学可询问的光学签名编码方案。 可以使用各种各样的改性底物，其提供用于以随机或图案分布容纳微球体的离散或非离散位点。 衬底可以由各种材料构成以形成二维或三维构型。 在优选的实施方案中，采用改性的光纤束或阵列作为基底以产生高密度阵列。 所公开的系统和方法具有检测目标分析物和筛选大型生物活性剂库的效用。

    Fiber optic sensor for long-term analyte measurements in fluids
    44.
    发明授权
    Fiber optic sensor for long-term analyte measurements in fluids 失效
    用于液体中长期分析物测量的光纤传感器

    公开(公告)号：US06285807B1

    公开(公告)日：2001-09-04

    申请号：US09192993

    申请日：1998-11-16

    Applicant: David R. Walt ,  Mary Beth Tabacco ,  Mahesh Uttamlal

    Inventor： David R. Walt ,  Mary Beth Tabacco ,  Mahesh Uttamlal

    IPC: G02B600

    CPC classification number: G01N21/8507 ,  G01N21/7703

    Abstract: A robust fiber optic sensor and sensing method for reliable, long-term measurement of analytes is disclosed. The sensor comprises an optical interrogation region comprising an indicator dye confined at a distal end of an optical fiber and an analyte permeable membrane enclosing the interrogation region at the distal fiber end. In a preferred embodiment, a reservoir member is provided with excess dye for continuous replenishment of the interrogation region with dye over the lifetime of the sensor. In another preferred embodiment, the reservoir member comprises an indicator support for containment of excess dye. In one preferred embodiment, a ratiometric dye is used for monitoring optical signal-to-noise and signal drift caused by sensor aging. The sensor may be configured with a variety of alternative indicator dyes and membrane materials as a specific ion sensor for analyzing dissolved analytes such as gases, cations, and anions. The sensor may be configured for a wide dynamic detection range and sensitivity for specific analytes. In one embodiment, a CO2 is disclosed which has a reversible working dynamic detection range between 200 and 1000 ppm pCO2 and a sensitivity ±1 ppm. An integrated measurement system and measurement methods for remote sensing applications are also disclosed which comprise electro-optic and data acquisition modules coupled to a conventional satellite transmission system. The sensor and sensing methods of the present invention have utility in remote sensing of analytes for environmental, industrial, chemical, biochemical, and biological monitoring of gases and liquids.

    Abstract translation: 公开了一种用于可靠，长期测量分析物的坚固的光纤传感器和感测方法。 传感器包括光学询问区域，其包含限定在光纤远端的指示剂染料和在远端光纤端部处包围询问区域的分析物可渗透膜。 在一个优选实施例中，储存器件具有多余的染料，用于在传感器寿命期间用染料连续补充询问区域。 在另一优选实施例中，储存器构件包括用于容纳过量染料的指示器支撑件。 在一个优选实施例中，使用比例染料来监测由传感器老化引起的光信号噪声和信号漂移。 传感器可以配置有各种替代的指示剂染料和膜材料作为用于分析溶解的分析物例如气体，阳离子和阴离子的特定离子传感器。 传感器可以配置为针对特定分析物的宽动态检测范围和灵敏度。 在一个实施方案中，公开了具有200至1000ppm pCO 2和灵敏度±1ppm之间的可逆工作动态检测范围的CO 2。 还公开了用于遥感应用的集成测量系统和测量方法，其包括耦合到常规卫星传输系统的电光和数据采集模块。 本发明的传感器和感测方法在用于气体和液体的环境，工业，化学，生化和生物监测的分析物的遥感中具有实用性。

    Flourescence intramolecular energy transfer conjugate compositions and detection methods
    47.
    发明授权
    Flourescence intramolecular energy transfer conjugate compositions and detection methods 失效
    荧光分子内能量转移共轭物组成和检测方法

    公开(公告)号：US5254477A

    公开(公告)日：1993-10-19

    申请号：US762245

    申请日：1991-09-19

    Applicant: David R. Walt

    Inventor： David R. Walt

    IPC: C12Q1/00 ,  G01N21/64 ,  G01N21/80

    CPC classification number: C12Q1/00 ,  G01N21/64 ,  G01N21/80 ,  Y10T436/144444

    Abstract: A variety of conjugated compositions and methods for the detection of an analyte of interest in a fluid sample is provided which relies upon an intramolecular energy transfer between a conjugated fluorophore and a chromophoric light-absorbing ligand for qualitative and quantitative results. The detection methods preferably employ fiber optic sensors in combination with analyte-insensitive fluorophores and analyte-sensitive absorber ligands in conjugated form. The methods and compositions rely upon the ability of the absorbing ligands to absorb energy which is transferred non-radiatively by the fluorophore when in an excited state.

    Abstract translation: 提供了用于检测流体样品中感兴趣分析物的各种共轭组合物和方法，其依赖于共轭荧光团和发色光吸收配体之间的分子内能量转移，用于定性和定量结果。 检测方法优选采用光纤传感器与分析物不敏感的荧光团和缀合形式的分析物敏感的吸收剂配体组合。 该方法和组合物依赖于吸收配体在处于激发态时吸收由荧光团非辐射转移的能量的能力。

    CO.sub.2 indicator and the use thereof to evaluate placement of tracheal tubes
    48.
    发明授权
    CO.sub.2 indicator and the use thereof to evaluate placement of tracheal tubes 失效
    二氧化碳指标及其用途来评估气管插管

    公开(公告)号：US5005572A

    公开(公告)日：1991-04-09

    申请号：US303952

    申请日：1989-01-31

    Applicant: Daniel B. Raemer ,  David R. Walt ,  Christianne Munkholm

    Inventor： Daniel B. Raemer ,  David R. Walt ,  Christianne Munkholm

    IPC: A61M16/04 ,  A61M16/08 ,  G01N31/22

    CPC classification number: G01N31/223 ,  A61M16/0488 ,  A61M16/085 ,  A61M2016/0413 ,  A61M2230/202

    Abstract: The invention relates to a CO.sub.2 detector for the detection of CO.sub.2 in respiratory gases. In particular, the invention relates to medical devices which contain a CO.sub.2 detector disposed within the devices to allow detection of CO.sub.2 within respiratory gases.The invention also relates to a method for determining the proper placement of an intubation device comprising inserting a tracheal tube having a CO.sub.2 detector disposed within, and observing whether the CO.sub.2 detector changes color in response to the CO.sub.2 which is present in respiratory gases.The CO.sub.2 detectors of the invention comprise a pH sensitive dye and a solid phase support. The CO.sub.2 detectors of the invention may further comprise a phase transport enhancer which allows the CO.sub.2 detector to respond contemporaneously with the presence or absence of CO.sub.2 in a respiratory gas on a breath by breath basis.

    Abstract translation: 本发明涉及一种用于检测呼吸气体中的二氧化碳的二氧化碳检测器。 特别地，本发明涉及包含设置在装置内的CO 2检测器以允许在呼吸气体中检测CO 2的医疗装置。 本发明还涉及一种用于确定插管装置的适当放置的方法，包括插入具有设置在其内的CO 2检测器的气管管，并观察CO 2检测器是否响应于呼吸气体中存在的CO 2而变色。 本发明的CO 2检测器包括pH敏感染料和固相支持物。 本发明的CO 2检测器还可以包括相转移增强剂，其允许CO 2检测器在呼吸气体呼吸气体中同时响应CO 2的存在或不存在。

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