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    METHOD AND DEVICE FOR DETERMINATIONS HYDROGEN PEROXIDE
    41.
    发明专利
    METHOD AND DEVICE FOR DETERMINATIONS HYDROGEN PEROXIDE 失效

    公开(公告)号：JPS57173744A

    公开(公告)日：1982-10-26

    申请号：JP5901981

    申请日：1981-04-18

    Applicant: ORIENTAL YEAST CO LTD

    Inventor： KEIDA MASAHIRO ,  ITOU YOSHIO ,  TOYODA MASATAKE ,  UTSUKI YOSHIO ,  OOHASHI MINORU ,  OKA OSAMU

    IPC: G01N27/416 ,  C12Q1/28

    Abstract: PURPOSE:To rapidly and simply measure H2O2 in a minute amount of 1ppm or less, by a method wherein, after sample liquid is treated by a treating liquid containing an oxidizer and is deoxidized, a detection is made on oxygen produced through implanting of catalase. CONSTITUTION:A specified amount of a solid sample is placed in a homogenizer, and is equalized by adding treating liquid, obtained resulting from filteration of the treating liquid, is poured into a receptacle 2, N2 gas is led into measuring liquid 2' in the reaction receptacle 2 and in an upper space 2'' of the reaction receptacle 2 through a 4-way cock 7 for switching N2 gas to exhaust all oxygen in the reaction receptacle 2. Then, the switch cock 7 is switched to a flow path shown in a dashed line to lead N2 gas only to the upper space 2'' in the reaction receptacle 2. A catalase solution, previously deoxidizing, is measured into a syringe 15 from a storage tank 14, and the catalase solution is implanted to measuring liquid through insertion of the syringe 15 into a cover. The implatation detects dissolved oxygen, generated in the measuring liquid, by means of an oxygen electrode 1 to find the H2O2 concentration of the measuring liquid.

    HIGHLY SENSITIVE MEASURING METHOD FOR CONCENTRATION OF HYDROGEN PEROXIDE
    42.
    发明专利
    HIGHLY SENSITIVE MEASURING METHOD FOR CONCENTRATION OF HYDROGEN PEROXIDE 失效

    公开(公告)号：JPS56128452A

    公开(公告)日：1981-10-07

    申请号：JP3166880

    申请日：1980-03-14

    Applicant: ORIENTAL YEAST CO LTD

    Inventor： UTSUKI YOSHIO ,  OOHASHI MINORU ,  OKA OSAMU

    IPC: G01N27/416 ,  G01N31/00

    Abstract: PURPOSE:To quantitatively measure a very small amoung of H2O2 in the order of ppb, by using a detecting element of exygen closely stuck a fixation membrane of decomposition agent of hydrogen peroxide to detecting face and continuously blowing an inactive gas in the liquid to be inspected in order to exclude dissolved oxygen. CONSTITUTION:The fixation membrane 2 of H2O2 decomposition catalyst composed of e.g. catalase or living body microorganism fungus containing catalase, or oxides of iron, manganese, cobalt etc. or organic complex salts, is furnished on detecting face of polarograph type oxygen electrode 1 and the electrode 1 is attached on the side wall of the reaction vessel 3. The base solution 20 of phosphoric acid buffer being suitable for the progress of H2O2 decomposition reaction, is put in the vessel 3 and dissolved oxygen is removed by supplying N2 gas from the inactive gas source 6. Oxygen in the sample 30 is removed by N2 gas preliminarily. H2O2 in the sample is decomposed by the membrane 2 and generating volume of oxygen is detected by the electrode 1 and then, its increased volume is recorded by the recorder 9 and also, increasing velocity is detected through the differentiation circuit 8.

    DETERMINATION OF AMYLASE ACTIVITY
    43.
    发明专利
    DETERMINATION OF AMYLASE ACTIVITY 失效

    公开(公告)号：JPS5534008A

    公开(公告)日：1980-03-10

    申请号：JP10395478

    申请日：1978-08-28

    Applicant: ORIENTAL YEAST CO LTD

    Inventor： FUJITA TAKESHI ,  OKA OSAMU ,  KOUKAWARA ISAMU

    IPC: C12Q1/40

    Abstract: PURPOSE:To determine the amylase activity with ease and accuracy, by converting maltose and glucose in the specimen with an enzyme system into 6-phosphogluconic acid, and by reacting the acid with dextrin, etc. and NAD to increase the NADH. CONSTITUTION:An enzyme system comprising maltase, hexokinase, glucose-6- phosphate dehydrogenase, an enzyme for oxidizing NAD and NADPH, and a substrate for oxidizing NADPH is added to remove the maltose and (or) glucose from the specimen. The maltose and glucose are converted into 6-phosphogluconic acid. One or more types of starch, dextrin, amylose, amylopectin in excess, and NAD are reacted with the acid to determine the amylase activity from the increase in NADH with an abosrbance at 340nm.

    Highly biotin-containing yeast and method for producing the same
    44.
    发明专利
    Highly biotin-containing yeast and method for producing the same 有权
    高生物含量的YEAST及其生产方法

    公开(公告)号：JP2008054606A

    公开(公告)日：2008-03-13

    申请号：JP2006236886

    申请日：2006-08-31

    Applicant: Oriental Yeast Co Ltd ,  オリエンタル酵母工業株式会社

    Inventor： NONOBE MASATSUGU ,  MASUDA YOSHIFUMI ,  OKA OSAMU

    IPC: C12N1/16 ,  A23L1/28

    Abstract: PROBLEM TO BE SOLVED: To provide a highly biotin-containing yeast which contains biotin absorbed in high concentration and little has the odor of the yeast, and to provide a method for producing the same, especially a method for producing the highly biotin-containing yeast, comprising finding culture conditions for inhibiting the proliferation of yeast and simultaneously remarkably absorbing the biotin, and then culturing the yeast under the conditions. SOLUTION: This method for producing the highly biotin-containing yeast is characterized by dissolving biotin in a solvent, preferably an alkaline solvent, adding the solution to a carbon source-free yeast culture solution, adjusting the pH of the yeast culture solution on the acidic side, and then culturing the yeast culture solution at a high temperature. COPYRIGHT: (C)2008,JPO&INPIT

    Abstract translation: 待解决的问题：为了提供高生物素的酵母，其含有高浓度吸收的生物素，并且几乎没有酵母的气味，并且提供其生产方法，特别是生产高生物素的方法 包括发现用于抑制酵母增殖并同时显着吸收生物素的培养条件，然后在条件下培养酵母。 解决方案：用于生产高生物素的酵母的方法的特征在于将生物素溶解在溶剂中，优选碱性溶剂中，将该溶液加入到不含碳源的酵母培养液中，调节酵母培养液的pH 在酸性方面，然后在高温下培养酵母培养液。 版权所有（C）2008，JPO＆INPIT

    New seasoning
    45.
    发明专利
    New seasoning 有权
    新季节

    公开(公告)号：JP2007097488A

    公开(公告)日：2007-04-19

    申请号：JP2005291763

    申请日：2005-10-04

    Applicant: Nisshin Flour Milling Inc ,  Oriental Yeast Co Ltd ,  オリエンタル酵母工業株式会社 ,  日清製粉株式会社

    Inventor： YAMAMOTO EIJI ,  INDO KAORU ,  OSADA SADAO ,  IBARADA KAZUMASA ,  HAMADA KAZUHIRO ,  NAKAJIMA RYOICHI ,  OKA OSAMU ,  NAGASHIMA AKIHIRO

    IPC: A23L27/00 ,  A23L27/10 ,  A23L27/24

    Abstract: PROBLEM TO BE SOLVED: To provide new seasoning excellent in balance between body taste and deliciousness and their addition effect, having reduced or removed yeast smell in spite of containing yeast extract, and having excellent flavor improving effect. SOLUTION: The seasoning contains 90-50 pts.mass of pulverized yeast extract, and 10-50 pts.mass of pulverized brewed seasoning. Especially, the yeast extract is preferably yeast extract which is produced by a self-digestion method, and the brewed seasoning is suitably used together with brewed seasoning which is produced using gluten and wheat flour as the main raw material. COPYRIGHT: (C)2007,JPO&INPIT

    Abstract translation: 要解决的问题：为了提供身体味道和美味之间的平衡优异的新调味料及其添加效果，尽管含有酵母提取物，但具有降低或除去酵母气味，并且具有优异的风味改善效果。

    解决方案：调味料含有90-50 pts.mass的粉碎酵母提取物和10-50 pts.mass的粉碎调味料。 特别地，酵母提取物优选是通过自消化法生产的酵母提取物，并且将酿造的调味料与以麸质和小麦粉作为主要原料制成的酿造调味料一起适用。 版权所有（C）2007，JPO＆INPIT

    ASSAY OF ARTHRITIC MELANOTRANSFERRIN AND REAGENT THEREFOR
    47.
    发明专利
    ASSAY OF ARTHRITIC MELANOTRANSFERRIN AND REAGENT THEREFOR 失效

    公开(公告)号：JP2001050964A

    公开(公告)日：2001-02-23

    申请号：JP22256899

    申请日：1999-08-05

    Applicant: ORIENTAL YEAST CO LTD

    Inventor： KATO YUKIO ,  MATSUKAWA HIROKAZU ,  YOSHIHARA YUTAKA ,  OKA OSAMU ,  FUJITA TAKESHI

    IPC: G01N33/564 ,  G01N33/50 ,  G01N33/53 ,  G01N33/68

    Abstract: PROBLEM TO BE SOLVED: To diagnose and detect arthritis, including discrimination thereof, in the early stage by measuring the concentration of melanotransferrin(MTF) in a biological sample by a method utilizing an antibody exhibiting high specificity to MTF. SOLUTION: First and second anti-MTF monoclonal antibodies are utilized in the measurement of concentration of MTF contained in articulation fluid or blood serum. An assay plate, where a microplate is coated with the first anti-MTF monoclonal antibody is used and a second biotin anti-MTF monoclonal antibody, is used as a second antibody in a specified sandwich type enzyme immunoassay for measuring the MTF concentration of articulation fluid of an arthritic patient. A reagent kit for use in this method is also provided. MTF in articulation fluid is significantly high in the case of arthritis and a significant difference appears in the distribution of MTF concentration in case of arthritis, arthritis rheumatica or traumatic injury of articulation.

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