公开(公告)号：US20230175965A1

公开(公告)日：2023-06-08

申请号：US18080481

申请日：2022-12-13

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Yi Zhang ,  Cheng Zong

IPC: G01N21/64 ,  G01N33/50 ,  C12Q1/04

CPC classification number: G01N21/6458 ,  G01N21/6428 ,  G01N33/5044 ,  C12Q1/04 ,  G01N2021/655

Abstract: Microscopic analysis of a sample includes a fluorescent dye disposed within the sample. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. An optical source generates a probe beam directed to impinge on the sample. A detector detects fluorescent emissions from the sample when the probe beam impinges on the sample. A data acquisition and processing system acquires and processes the detected fluorescent emissions from the sample to: (i) generate a signal indicative of infrared absorption by the sample, (ii) generate a signal indicative of temperature in the sample based on the signal indicative of infrared absorption by the sample, (iii) generate an image of the sample using the signal indicative of temperature in the sample.

公开(公告)号：US20230039153A1

公开(公告)日：2023-02-09

申请号：US17839080

申请日：2022-06-13

Applicant: Trustees of Boston University ,  Northwestern University

Inventor： Ji-Xin Cheng ,  Junjie Li ,  Yuying Tan ,  Daniela Matei ,  Guangyuan Zhao

IPC: G01N33/49 ,  G01N27/416 ,  G01Q60/60 ,  G01N27/30 ,  G16H20/10 ,  G16H50/30 ,  G01N33/487

Abstract: Disclosed is an assay for determining resistance in a target cell or tissue to a therapy associated with cellular stress using chemical microscopy and high-throughput single cell analysis to determine functional metabolic alteration, including determining metabolic reprogramming in a target cell or tissue to a therapy associated with cellular stress, and methods of using the assays.

公开(公告)号：US11561179B2

公开(公告)日：2023-01-24

申请号：US17466672

申请日：2021-09-03

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Yi Zhang ,  Cheng Zong

IPC: G01N21/64 ,  G01N33/50 ,  C12Q1/04 ,  G01N21/65

Abstract: Microscopic analysis of a sample includes a fluorescent dye disposed within the sample. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. An optical source generates a probe beam directed to impinge on the sample. A detector detects fluorescent emissions from the sample when the probe beam impinges on the sample. A data acquisition and processing system acquires and processes the detected fluorescent emissions from the sample to: (i) generate a signal indicative of infrared absorption by the sample, (ii) generate a signal indicative of temperature in the sample based on the signal indicative of infrared absorption by the sample, (iii) generate an image of the sample using the signal indicative of temperature in the sample.

公开(公告)号：US20220074861A1

公开(公告)日：2022-03-10

申请号：US17466672

申请日：2021-09-03

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Yi Zhang ,  Cheng Zong

IPC: G01N21/64 ,  C12Q1/04 ,  G01N33/50

Abstract: Microscopic analysis of a sample includes a fluorescent dye disposed within the sample. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. An optical source generates a probe beam directed to impinge on the sample. A detector detects fluorescent emissions from the sample when the probe beam impinges on the sample. A data acquisition and processing system acquires and processes the detected fluorescent emissions from the sample to: (i) generate a signal indicative of infrared absorption by the sample, (ii) generate a signal indicative of temperature in the sample based on the signal indicative of infrared absorption by the sample, (iii) generate an image of the sample using the signal indicative of temperature in the sample.