公开(公告)号：US12298238B2

公开(公告)日：2025-05-13

申请号：US18234195

申请日：2023-08-15

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Lu Lan ,  Jiaze Yin

IPC: G01N21/359 ,  G01N21/64 ,  G01N21/65

Abstract: A mid-infrared photothermal microscopy system images a sample. A mid-infrared optical source generates a mid-infrared beam which is directed along a first optical path to reach the substrate on a first side and heat the sample. A probe light source generates a probe light which is directed along a second optical path to reach the substrate on a second side and illuminate the sample. A first laser scanner is positioned along the first optical path and configured to rotate to redirect light and scan the sample with the mid-infrared beam. A second laser scanner is positioned along the second optical path and configured to rotate to redirect light and scan the sample with the probe light. The laser scanners each include at least one mirror driven to rotate such that the mid-infrared beam and the probe light scan the sample synchronously.

公开(公告)号：US20250102437A1

公开(公告)日：2025-03-27

申请号：US18923191

申请日：2024-10-22

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Yi Zhang ,  Cheng Zong

IPC: G01N21/64 ,  C12Q1/04 ,  G01N21/65 ,  G01N33/50

Abstract: Microscopic analysis of a sample includes a fluorescent dye disposed within the sample. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. An optical source generates a probe beam directed to impinge on the sample. A detector detects fluorescent emissions from the sample when the probe beam impinges on the sample. A data acquisition and processing system acquires and processes the detected fluorescent emissions from the sample to: (i) generate a signal indicative of infrared absorption by the sample, (ii) generate a signal indicative of temperature in the sample based on the signal indicative of infrared absorption by the sample, (iii) generate an image of the sample using the signal indicative of temperature in the sample.

公开(公告)号：US20250012695A1

公开(公告)日：2025-01-09

申请号：US18761564

申请日：2024-07-02

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Qing Xia

IPC: G01N15/01 ,  G01N15/1434

Abstract: A wide-field microscopy system and method for imaging a sample include directing infrared light onto the sample to selectively heat the sample. Probe light is also directed onto the sample. An objective collects the probe light after it interacts with the sample. The collected probe light is detected at a detector. A relative distance between the objective and sample is adjusted to introduce an optical defocus enhancement to enhance detection of a change in detected probe light that is indicative of infrared absorption by the sample.

公开(公告)号：US12152990B2

公开(公告)日：2024-11-26

申请号：US18080481

申请日：2022-12-13

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Yi Zhang ,  Cheng Zong

IPC: G01N21/64 ,  C12Q1/04 ,  G01N21/65 ,  G01N33/50

Abstract: Microscopic analysis of a sample includes a fluorescent dye disposed within the sample. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. An optical source generates a probe beam directed to impinge on the sample. A detector detects fluorescent emissions from the sample when the probe beam impinges on the sample. A data acquisition and processing system acquires and processes the detected fluorescent emissions from the sample to: (i) generate a signal indicative of infrared absorption by the sample, (ii) generate a signal indicative of temperature in the sample based on the signal indicative of infrared absorption by the sample, (iii) generate an image of the sample using the signal indicative of temperature in the sample.

公开(公告)号：US20240337589A1

公开(公告)日：2024-10-10

申请号：US18630750

申请日：2024-04-09

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Lu Lan ,  Jiaze Yin

IPC: G01N21/17 ,  G01N21/35 ,  G01N21/47

CPC classification number: G01N21/171 ,  G01N21/35 ,  G01N21/47 ,  G01N2021/3595

Abstract: Systems and methods are provided for performing photothermal dynamic imaging. An exemplary method includes: scanning a sample to produce a plurality of raw photothermal dynamic signals; receiving the raw photothermal dynamic signals of the sample; generating a plurality of second signals by matched filtering the raw photothermal dynamic signals to reject non-modulated noise; and performing an inverse operation on the second signals to retrieve at least one thermodynamic signal in a temporal domain.

公开(公告)号：US20230408805A1

公开(公告)日：2023-12-21

申请号：US18205152

申请日：2023-06-02

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Celalettin Yurdakul ,  Haonan Zong ,  M. Selim Ünlü

IPC: G02B21/16 ,  G02B21/02 ,  G02B21/36

CPC classification number: G02B21/16 ,  G02B21/025 ,  G02B21/361

Abstract: Microscopic analysis of a sample includes a system using dark-field illumination. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. A visible light source generates a light illuminating the sample on a substrate and creating a scattered field and a reflected field along a collection path of the system. A pupil mask is positioned along the collection path to block the reflected field while allowing the scattered field to pass therethrough. A camera is positioned at an end of the collection path to collect the scattered field and generate a dark-field image of the sample.

公开(公告)号：US20230384288A1

公开(公告)日：2023-11-30

申请号：US18317637

申请日：2023-05-15

Applicant: Trustees of Boston University ,  Northwestern University

Inventor： Ji-Xin Cheng ,  Junjie Li ,  Yuying Tan ,  Daniela Matei ,  Guangyuan Zhao

IPC: G01N33/49 ,  G01Q60/60 ,  G01N27/30 ,  G16H20/10 ,  G01N27/416 ,  G01N33/487 ,  G16H50/30 ,  G01N21/65

CPC classification number: G01N33/492 ,  G01Q60/60 ,  G01N27/30 ,  G16H20/10 ,  G01N21/65 ,  G01N33/48735 ,  G16H50/30 ,  G01N2800/52 ,  G01N27/416

Abstract: Disclosed is an assay for determining resistance in a target cell or tissue to a therapy associated with cellular stress using chemical microscopy and high-throughput single cell analysis to determine functional metabolic alteration, including determining metabolic reprogramming in a target cell or tissue to a therapy associated with cellular stress, and methods of using the assays.

公开(公告)号：US11774365B2

公开(公告)日：2023-10-03

申请号：US17569966

申请日：2022-01-06

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Haonan Lin

IPC: G06T5/00 ,  G01N21/65 ,  G06N3/04 ,  G06N3/08 ,  A61B5/00

CPC classification number: G01N21/65 ,  G06N3/04 ,  G06N3/08 ,  G06T5/001 ,  A61B5/0075 ,  G01N2021/655 ,  G06T2207/20081 ,  G06T2207/20084

Abstract: Systems and methods implement of high-speed delay scanning for spectroscopic SRS imaging characterized by scanning a first pulsed beam across a stepwise reflective surface (such as a stepwise mirror or a reflective blazed grating) in a Littrow configuration to generate near continuous temporal delays relative to a second pulsed beam. Systems and methods also implement deep learning techniques for image restoration of spectroscopic SRS images using a trained encoder-decoder convolution neural network (CNN) which in some embodiments may be designed as a spatial-spectral residual net (SS-ResNet) characterized by two parallel filters including a first convolution filter on the spatial domain and a second convolution filter on the spectral domain.

公开(公告)号：US11564742B2

公开(公告)日：2023-01-31

申请号：US17737710

申请日：2022-05-05

Applicant: Trustees of Boston University

Inventor： Chen Yang ,  Ji-Xin Cheng ,  Nan Zheng ,  Yueming Li ,  Ying Jiang ,  Lu Lan ,  Carolyn Marar

IPC: A61B18/18 ,  H01P7/08

Abstract: A system for neuromodulation includes a split-ring resonator (SRR) comprising a resonance circuit, the SRR being implantable in a cranial target site and a source of microwave signals, wherein the microwave signals are deliverable wirelessly to couple with the SRR to produce a localized electrical field, wherein the localized electrical field inhibits one or more neurons at the cranial target site with submillimeter spatial precision.

公开(公告)号：US20220354573A1

公开(公告)日：2022-11-10

申请号：US17737710

申请日：2022-05-05

Applicant: Trustees of Boston University

Inventor： Chen Yang ,  Ji-Xin Cheng ,  Nan Zheng ,  Yueming Li ,  Ying Jiang ,  Lu Lan ,  Carolyn Marar

IPC: A61B18/18 ,  H01P7/08

Abstract: A system for neuromodulation includes a split-ring resonator (SRR) comprising a resonance circuit, the SRR being implantable in a cranial target site and a source of microwave signals, wherein the microwave signals are deliverable wirelessly to couple with the SRR to produce a localized electrical field, wherein the localized electrical field inhibits one or more neurons at the cranial target site with submillimeter spatial precision.