公开(公告)号：JPH06220095A

公开(公告)日：1994-08-09

申请号：JP904193

申请日：1993-01-22

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KUBO FUMIO ,  KUDO ISAO ,  TANAKA MASAYUKI ,  MIWA TOSHIYUKI

IPC: C07K14/435 ,  C07K1/00 ,  C07K1/16 ,  C07K15/06 ,  C07K3/18

Abstract: PURPOSE:To separate E.coli protein and human physiologically active protein from an aqueous solution containing E.coli protein obtained by the disintegration of E.coli and human physiologically active protein by using a resin bonded with alkyl group. CONSTITUTION:An E.coli protein and a physiologically active protein such as human SOD are separated from a 50mM buffer solution of pH 7.0 containing E.coli protein obtained by the disintegration of gene-recombinant E.coli and human SOD using an alkyl-bonded resin such as Butyl Toyopearl 650M.

公开(公告)号：JPH06209771A

公开(公告)日：1994-08-02

申请号：JP904093

申请日：1993-01-22

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KUBO FUMIO ,  TANAKA MASAYUKI ,  IWAMOTO YOSHITADA

IPC: C12N9/02 ,  C12N15/09 ,  C12N15/10 ,  C12P21/00 ,  C12R1/19

Abstract: PURPOSE:To separate, from a solution containing a DNA obtained by cell homogenization of a microorganism and a biologically active protein, the DNA and the biologically active protein by using a resin bound with anion exchanging groups. CONSTITUTION:An E. coli DNA and a biologically active protein such as human SOD are separated by using a resin bound with anion exchanging groups such as HPA-25 from a buffer solution of 10mM and pH7.0 containing the DNA obtained by cell homogenization of recombinant E. coli. and the biologically active protein.