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公开(公告)号：JPH06217768A
公开(公告)日：1994-08-09
申请号：JP1097993
申请日：1993-01-26
Applicant: UBE INDUSTRIES
Inventor： FUKUI KIYOSHI , KUBO FUMIO , KUDO ISAO , UMEDA KAZUHIRO , IWAMOTO YOSHITADA , OKA YURIO
Abstract: PURPOSE:To produce human copper, zinc type super oxide dismutase containing no copper or deficient in copper and useful as a medicine by culturing a microbial cell having human copper, zinc type super oxide dismutase-producing ability. CONSTITUTION:Yield of human copper and zinc type super oxide dismutase containing no copper or deficient in copper to microbial cell amount can remarkably be enhanced by adding Escherichia coli having human copper, zinc type super oxide dismutase producing ability to 90,000ml medium containing K2HPO4, KH2PO4, Na2HPO4, soybean protein hydrolyzate, methionine, tryptophan, aspartic acid, glucose, MgSO4, CaCl2.2H2O, MnSO4, AlCl3, H3BO3, FeSO4, CuCl2.2H2O, ZnSO4.7H2O and tetracycline and culturing the bacterium while adding 10.71g ZnSO4.7H2O thereto in three portions during culture.
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公开(公告)号：JPH06220095A
公开(公告)日：1994-08-09
申请号：JP904193
申请日：1993-01-22
Applicant: UBE INDUSTRIES
Inventor： FUKUI KIYOSHI , KUBO FUMIO , KUDO ISAO , TANAKA MASAYUKI , MIWA TOSHIYUKI
IPC: C07K14/435 , C07K1/00 , C07K1/16 , C07K15/06 , C07K3/18
Abstract: PURPOSE:To separate E.coli protein and human physiologically active protein from an aqueous solution containing E.coli protein obtained by the disintegration of E.coli and human physiologically active protein by using a resin bonded with alkyl group. CONSTITUTION:An E.coli protein and a physiologically active protein such as human SOD are separated from a 50mM buffer solution of pH 7.0 containing E.coli protein obtained by the disintegration of gene-recombinant E.coli and human SOD using an alkyl-bonded resin such as Butyl Toyopearl 650M.
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Patent Agency Ranking
公开(公告)号：JPH0522509B2
公开(公告)日：1993-03-29
申请号：JP3192887
申请日：1987-02-14
Applicant: UBE INDUSTRIES
Inventor： FUKUI KYOSHI , KUBO FUMIO , WATANABE MASAYUKI
IPC: C12N9/02
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公开(公告)号：JPH06217762A
公开(公告)日：1994-08-09
申请号：JP1151093
申请日：1993-01-27
Applicant: UBE INDUSTRIES
Inventor： FUKUI KIYOSHI , KUBO FUMIO , KUDO ISAO , UMEDA KAZUHIRO , IWAMOTO YOSHITADA , OKA YURIO
Abstract: PURPOSE:To provide a high-concentration and high-efficiency culture method for coli bacillus, capable of efficiently producing the objective substance in production of an enzyme, a bioactive substance, etc., by coli bacillus. CONSTITUTION:In relation to aerobic culture of coli bacillus, this high- concentration and high-efficiency culture method is carried out by adding an amino acid composition to the culture medium so that amino acids required for growth of the coli bacillus may not run short and simultaneously adding glucose to the medium so as to control pH of the medium to be a constant value in a state where pH of the medium increases.
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公开(公告)号：JPH06209771A
公开(公告)日：1994-08-02
申请号：JP904093
申请日：1993-01-22
Applicant: UBE INDUSTRIES
Inventor： FUKUI KIYOSHI , KUBO FUMIO , TANAKA MASAYUKI , IWAMOTO YOSHITADA
Abstract: PURPOSE:To separate, from a solution containing a DNA obtained by cell homogenization of a microorganism and a biologically active protein, the DNA and the biologically active protein by using a resin bound with anion exchanging groups. CONSTITUTION:An E. coli DNA and a biologically active protein such as human SOD are separated by using a resin bound with anion exchanging groups such as HPA-25 from a buffer solution of 10mM and pH7.0 containing the DNA obtained by cell homogenization of recombinant E. coli. and the biologically active protein.
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公开(公告)号：JPS63198983A
公开(公告)日：1988-08-17
申请号：JP3192887
申请日：1987-02-14
Applicant: UBE INDUSTRIES
Inventor： FUKUI KIYOSHI , KUBO FUMIO , WATANABE MASAYUKI
IPC: C12N9/02
Abstract: PURPOSE:To produce recombinant human superoxide dismutase (SOD) containing theoretical amount of copper ion and having high enzyme activity, by treating a solution of apoenzyme containing no copper of recombinant human SOD or insufficient for the copper with a copper salt in the presence of beta- mercaptoethanol. CONSTITUTION:A microorganism transformed with recombinant DNA having a human superoxide dismutase (SOD) structural gene downstream plasma expressing regulatory gene is cultivated and induction synthesis of SOD is carried out to provide a humid germ cell, which is suspended in a proper water solution having 5-8pH, ground to afford supernatant. beta-Mercaptoethanol having 5-100mM concentration is added to the supernatant or supernatant, etc., obtained after treating precipitation of ammonium sulfate, and further a copper salt having anion of an organic or inorganic acid is added in a concentration of 0.15X10 -15X10 mol. based on 1g of the humid germ cell at 0 deg.C - room temperature and the mixture is allowed to stand or stirred at 0 deg.C - room temperature, preferably at low temperature of nearly 4 deg.C for 10min - 1 day to isolate the aimed SOD.
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公开(公告)号：JPH06199895A
公开(公告)日：1994-07-19
申请号：JP202593
申请日：1993-01-08
Applicant: UBE INDUSTRIES
Inventor： FUKUI KIYOSHI , KUBO FUMIO , TANAKA MASAYUKI , IWAMOTO YOSHITADA
Abstract: PURPOSE:To obtain the subject derivative useful for treating rheumatoid arthritis, cataract, etc., having uniform electric charge and molecular weight and stable activity by reacting a human Cu, Zn type superoxide dismutase with a specific alkyl disulfide. CONSTITUTION:Human Cu, Zn type superoxide dismutase is mixed with an alkyl disulfide of formula I (X is amino, 1-10C alkyl, amino replaced with 1-10C alkyl or 2-10C acyl group or hydroxyl; R and R are H or 1-IOC alkyl), adjusted to pH 7.0, incorporated with water and reacted at 4 deg.C for 24 hours while stirring to give the objective enzyme derivative of formula II (SOD' is group obtained by removing H from mercapto group of cystine residue at 111st of human Cu, Zn type superoxide dismutase) having uniformity with respect to electronic charge and molecular weight, stable activity, useful as a preventive and a therapeutic agent for rheumatoid arthritis, oxygen retinopathy of immature baby, cataract, etc., in which superoxide exists.
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