公开(公告)号：WO1996032198A1

公开(公告)日：1996-10-17

申请号：PCT/US1996005144

申请日：1996-04-12

Applicant: COBE LABORATORIES, INC.

Inventor： COBE LABORATORIES, INC. ,  HLAVINKA, Dennis ,  FELT, Thomas, J.

IPC: B04B05/04

CPC classification number: B04B5/0442 ,  B04B2005/045

Abstract: A centrifuge apparatus is used for collecting white blood cells, primarily mononuclear cells, from whole blood stratified into layers. A thin mononuclear (MNC) layer is formed at the interface of red blood cells and plasma. A barrier is positioned in the separation vessel of the centrifuge at a location to intercept the thin layer. An MNC collect port is positioned in front of the barrier to collect the thin layer. MNC fluid is allowed to pool behind the barrier to surround the collect port before collection is started. Collection ceases when the pool is removed and allowed to build again. By operating the collect in an intermittent fashion, improvements in purity and collect volume are achieved. The intermittent collection procedure can be useful for harvesting granulocytes and, in general, any sparse stratified component of a centrifuged solution where the sparse component is layered between more dense and less dense strata.

Abstract translation: 离心机用于从分层成层的全血中收集白细胞，主要是单核细胞。 在红细胞和血浆的界面处形成薄单核（MNC）层。 隔离物位于离心机的分离容器中，以拦截薄层。 MNC收集端口位于屏障前面以收集薄层。 在收集开始之前，允许MNC流体在障碍物后面集中以围绕收集端口。 当池被移除并允许再次建立时，集合停止。 通过间歇操作收集，实现了纯度和收集体积的提高。 间歇收集程序可用于收获粒细胞和一般来说，离心溶液的任何稀疏的分层成分，其中稀疏组分分层在更致密和较不密集的层之间。

公开(公告)号：WO1994022585A1

公开(公告)日：1994-10-13

申请号：PCT/US1994003596

申请日：1994-04-01

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MOORE, Patrick, Q. ,  SCHIESSLER, David, L.

IPC: B04B05/04

CPC classification number: B65D39/0088 ,  B01L3/5021 ,  B04B5/0414 ,  B65D2539/006

Abstract: The present invention directed to a plug (16) for sealing a centrifuge tube (10). The plug (16) of the present invention is shaped and sized to provide an interference fit between the plug (16) and the tube stem (12) of the centrifuge tube. In the described embodiment, the plug is configured with a tapered body narrowing to a flared end (22). To facilitate the insertion and removal of the plug with moderate force, without compromising the restraining capability of the interference fit, the area of interference contact between the flared end and the filler stem is strategically reduced. An o-ring is provided in an annular groove (20) around the tapered body. The flared end (22) creates an interference fit with a tapered filler stem (12) whereby the plug (16) is secured in the filler stem (12) with a snapping action when the flared end (22) of the plug (16) extends into the tube beyond the tapered filler stem (12). In this snap-in position, the plug (16) is secured in the filler stem (12), providing an initial seal; such seal increases upon centrifugation by a self-sealing mechanism, either attributed to the internal hydrostatic pressure in the tube and/or the force of a support spacer on the plug. After centrifugation, the snap coupling between the filler stem and the plug securely retains the plug against any residual internal pressure built up within the tube created by deformation of the centrifuge tube either from centrifugation or through handling of the tube by the user.

Abstract translation: 本发明涉及用于密封离心管（10）的塞子（16）。 本发明的插头（16）的形状和尺寸被设计成提供离心管的插塞（16）和管杆（12）之间的过盈配合。 在所描述的实施例中，插头构造成具有朝向扩张端（22）变窄的锥形体。 为了有利于以适度的力插入和移除插头，在不影响过盈配合的约束能力的情况下，战略上减少了扩口端与加注杆之间的干涉接触面积。 O形圈设置在围绕锥形主体的环形槽（20）中。 扩口端（22）产生与锥形填充杆（12）的过盈配合，由此当插头（16）的扩口端（22）具有卡扣动作时，插头（16）固定在填充杆（12） 延伸到超过锥形填充杆（12）的管中。 在该卡入位置中，插头（16）固定在加注杆（12）中，提供初始密封; 这种密封通过自密封机构进行离心时增加，归因于管中的内部静水压力和/或插塞上的支撑间隔件的力。 离心后，填充杆和插塞之间的卡扣联结可以牢固地保持插塞抵抗由离心管变形而产生的管内的任何残余内部压力，或者离心或通过使用者处理管。

公开(公告)号：WO1988006922A1

公开(公告)日：1988-09-22

申请号：PCT/US1988000869

申请日：1988-03-07

Applicant: DAMON CORPORATION

Inventor： DAMON CORPORATION ,  HOHENBERG, Rudolph ,  FIGDOR, Carl, G. ,  BONT, Willy, S.

IPC: B04B05/04

CPC classification number: B04B5/0428

Abstract: Apparatus for the centrifugal fractionation and component-isolation of liquid material has a nonflexing radially-movable pressure panel disposed between a first liquid container stacked radially in front of a second liquid container. The two containers are at the same interval pressure during centrifugation, and the pressure panel avoids radial distortion of the back wall of the first container. The liquid container structure can incorporate the pressure panel. Alternatively, the panel can be part of the centrifugal separating instrument.

公开(公告)号：WO1987001307A1

公开(公告)日：1987-03-12

申请号：PCT/NL1986000002

申请日：1986-01-13

Applicant: VERENIGING HET NEDERLANDS KANKER INSTITUUT ,  FIGDOR, Carl, G. ,  BONT, Willy, S.

Inventor： VERENIGING HET NEDERLANDS KANKER INSTITUUT

IPC: B04B05/04

CPC classification number: B04B9/14 ,  B04B5/0428 ,  B04B2009/143 ,  Y10T137/87917

Abstract: After separation and during centrifuging a separated blood component is transferred from a source reservoir (2) into a tube (4) oriented against a centrifugal gradient. A cap (34) converts the separated layer from the reservoir into a columnar body and the centrifugal gradient prevents mixing of the columnar body in the tube (4) during further centrifuging. In one embodiment the tube has a volume sufficient to hold a desired fractional component from the source reservoir, and a diameter small enough to prevent mixing of the component when the gradient is removed. A support (69) holds the conduit wound about a spool transverse to the iso-g-lines of the centrifuge. The support (69) may be removably affixed to the cap (34). In a preferred embodiment the source reservoir (2) has a flexible wall, and pressure of the wall against an elevation (29) maintains pressure in the fluid system. Flow blocking (7) and flow regulating means are shown for delivering small fractional components to the tube or to a receiving reservoir at a desired rate. In another embodiment a make-up fluid reservoir maintains fluid pressure and a peristaltic pump controls the transfer of separated components. A table with movable clamps (65-67) is also shown for isolating the fractions in the tube after centrifuging.

Abstract translation: 在分离之后和离心过程中，分离的血液成分从源储存器（2）转移到反对离心梯度取向的管（4）中。 盖（34）将分离的层从储存器转变为柱状体，并且离心梯度防止在进一步离心过程中管（4）中的柱状体的混合。 在一个实施例中，管具有足以保持来自源储存器的期望的分数分量的体积，以及直径足够小以防止当去除梯度时组分的混合。 支撑件（69）将导管缠绕在与离心机的等离子体线横向的线轴上。 支撑件（69）可以可移除地固定到盖子（34）上。 在优选实施例中，源储存器（2）具有柔性壁，壁相对于高度（29）的压力保持流体系统中的压力。 示出了流动阻塞（7）和流量调节装置，用于以期望的速率将小的分数分量输送到管或接收储存器。 在另一个实施例中，补充流体储存器保持流体压力，并且蠕动泵控制分离的部件的传送。 还显示了一个带有可移动夹具（65-67）的工作台，用于在离心后隔离管中的馏分。

公开(公告)号：WO1985005567A1

公开(公告)日：1985-12-19

申请号：PCT/US1985001028

申请日：1985-05-31

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  LAM, Kin, C. ,  WILLIAMS, Ronald, K.

IPC: B04B05/04

CPC classification number: B04B5/0414 ,  B04B2005/0435

Abstract: A centrifuge rotor (10) is provided with a plurality of cavities (20) having a non-circular cross-section for receiving mating multi-angle adapters (24), which may be inserted in the cavities (20) with selected rotational alignment about the cavity axes (m-m'), to determine a plurality of select angle receiving chambers (26) in the rotor (10) for sample containers.

公开(公告)号：WO1980002653A1

公开(公告)日：1980-12-11

申请号：PCT/SE1979000128

申请日：1979-06-06

Applicant: GAMBRO AB ,  LARSSON L ,  STENBERG K ,  GULLBERG C ,  BOBERG N

Inventor： GAMBRO AB

IPC: B04B05/04

CPC classification number: B04B5/0442

Abstract: Device for the centrifugal separation of a liquid, especially whole blood, into fractions having different densities. Said device comprises a rotatable separation unit (5) comprising a separation chamber (5'), and a transferring element (2) in fluid communication with said chamber The transferring of said liquid and said separated fractions between a source for said liquid and said separation chamber is realized without use of rotating couplings in that one end (3) of said transferring element is held fixedly, while the other end (4) thereof, i.e. the end which is next to said separation chamber, is rotatably connected to said chamber. As well said separation unit as said transferring element thereby will perform a rotation, when said separation chamber is rotating around an axis through said one end of said transferring element. Said transferring element (2) is thereby prevented from twisting or winding. More precisely, said transferring element (2) and said separation unit (5) will rotate one revolution (secondary rotation) per revolution made by the separation unit (5) when rotating (primary rotation) around said axis through said fixedly held end (3). By suitable choice of ratio between radius (r) of said separation chamber and radius (R) of said primary rotation, i.e. the distance between said separation chamber and the axis through said fixedly held end, said secondary rotation may be used to separate said liquid already before said liquid enters into said separation chamber (5').

Abstract translation: 用于将液体，特别是全血离心分离成具有不同密度的级分的装置。 所述装置包括可旋转分离单元（5），其包括分离室（5'）和与所述室流体连通的转移元件（2）。在所述液体源和所述分离器之间转移所述液体和所述分离的馏分 在不使用旋转联轴器的情况下实现腔室，其中所述传送元件的一端（3）被固定地保持，而其另一端（即，位于所述分离室旁边的端部）可旋转地连接到所述腔室。 同样地，当所述分离室围绕所述转移元件的所述一端的轴线旋转时，所述分离单元作为所述转移元件，从而将执行旋转。 从而防止所述转移元件（2）的扭转或卷绕。 更准确地说，当通过所述固定保持端（3）围绕所述轴线旋转（初级旋转）时，所述传送元件（2）和所述分离单元（5）将在由分离单元（5）制成的每转一圈（次级旋转） ）。 通过适当选择所述分离室的半径（r）和所述初级旋转的半径（R）之间的比率，即所述分离室与通过所述固定保持端的轴线之间的距离，所述次旋转可用于分离所述液体 已经在所述液体进入所述分离室（5'）之前。

公开(公告)号：EP0250825A3

公开(公告)日：1988-07-20

申请号：EP87107188

申请日：1987-05-18

Applicant: GREEN CROSS CORPORATION

Inventor： Takabayashi, Kuniaki ,  Inoue, Yoshiyuki ,  Shimotakahara, Masaki ,  Okada, Kikujiro ,  Shiino, Satoru

IPC: B04B05/04

CPC classification number: B04B5/0442

Abstract: There is disclosed a centrifugal separation case (1) assembly adapted to a continuous type centrifugal separation apparatus in which the twist of communication tube (15) used for injection of the liquid, for example the blood, and for discharge of the separated components such as the red blood cell etc. is positively prevented, further, the complete separation and the short time separation can be realized using the separation case (1), and the rigidity of the tubes (15, 16, 17, 18) provided for the injection and discharge is improved.

公开(公告)号：EP0245703A3

公开(公告)日：1988-07-13

申请号：EP87106226

申请日：1987-04-29

Applicant: MILES LABORATORIES, INC.

Inventor： Brenneman, Allen J. ,  Buck, Harvey B. Jr. ,  Pugh, Jerry T. ,  Robertson, Steven P.

IPC: B04B05/04 ,  B04B07/08

CPC classification number: B04B7/08 ,  B04B5/0407

Abstract: Separation device and method for the fractionation and separation of finely divided solid particulate material suspended in a liquid are disclosed. The separation device comprises. upper and lower portions which are joined together to form an inner cavity retaining liquid and a capillary gap which extends outwardly radially from said cavity. During rotation solid particles present in the suspending liquid pass outwardly along and/or become entrapped in the capillary gap(s).

公开(公告)号：EP0258652A3

公开(公告)日：1988-06-22

申请号：EP87111160

申请日：1987-08-01

Applicant: E.I. DU PONT DE NEMOURS AND COMPANY

Inventor： Weyant, Oakley Louis, Jr.

IPC: B04B05/04

CPC classification number: B04B5/0414 ,  B04B2007/025

Abstract: A centrifuge rotor has a continuous annular groove (38) disposed radially outwardly of each of the sample receiving cavities (18) provided in the rotor (10). The groove (38) collects any liquid spilled during operation of the rotor (10) thereby to prevent the liquid from draining into the cavities (18) and contaminating the same as the rotor (10) slows.

公开(公告)号：EP0222085A3

公开(公告)日：1988-06-08

申请号：EP86111809

申请日：1986-08-26

Applicant: Heraeus Separationstechnik GmbH

Inventor： Müller, Dieter ,  Hirsch, Christian ,  Pantucek, Peter, Dr.

IPC: B04B05/04

CPC classification number: B04B5/0421