公开(公告)号：KR1020130054497A

公开(公告)日：2013-05-27

申请号：KR1020110117784

申请日：2011-11-11

Applicant: 한국생명공학연구원 ,  대한민국(농촌진흥청장)

Inventor： 문제선 ,  이수헌 ,  김성욱 ,  임승모 ,  남문 ,  김정선 ,  최홍수 ,  이준성 ,  김정수

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/04

CPC classification number: C12Q1/701 ,  C12Q1/04 ,  C12Q1/6837

Abstract: PURPOSE: A probe set for diagnosing or detecting CPMMV(cowpea mild mottle virus) is provided to minimize damage due to viral diseases in crops and to enhance production. CONSTITUTION: An oilgonucleotide probe set for diagnosing or detecting CPMMV contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 578-587 and complementary oligonucleotides thereof. A method for diagnosing or detecting CPMMV comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA using the RNA as a template; a step of hybridizing the cDNA with the probe set; and a step of identifying the hybridization. [Reference numerals] (AA) Green pea; (BB) Host: Green pea

Abstract translation: 目的：提供用于诊断或检测CPMMV（cow豆轻度斑驳病毒）的探针，以尽量减少由于作物病毒性疾病引起的损伤，并提高产量。 构成：用于诊断或检测CPMMV的石油核苷酸探针组含有选自序列号578-587的寡核苷酸和其互补寡核苷酸的一种或多种寡核苷酸。 一种用于诊断或检测CPMMV的方法包括：从样品中分离RNA的步骤; 使用RNA作为模板合成cDNA的步骤; 将cDNA与探针组杂交的步骤; 以及鉴定杂交的步骤。 （附图标记）（AA）绿豆; （BB）主持人：绿豆

公开(公告)号：KR1020130054496A

公开(公告)日：2013-05-27

申请号：KR1020110117760

申请日：2011-11-11

Applicant: 한국생명공학연구원 ,  대한민국(농촌진흥청장)

Inventor： 문제선 ,  이수헌 ,  김성욱 ,  임승모 ,  남문 ,  김정선 ,  최홍수 ,  이준성 ,  김정수

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/04

CPC classification number: C12Q1/701 ,  C12Q1/04 ,  C12Q1/6837

Abstract: PURPOSE: A probe set for diagnosing or detecting PSV(peanut stunt virus) is provided to minimize damage due to viral diseases in crops and to enhance production. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting PSV contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 1025-1034, 1045-1049, and 2988, and complementary oligonucleotides thereof. A method for diagnosing or detecting PSV comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA; a step of hybridizing the cDNA with the probe set; and a step of detecting hybridization of the cDNA and the probe set. [Reference numerals] (AA) Cowpea; (BB) Host: cowpea; (CC) Kidney bean; (DD) Host: Kidney bean

Abstract translation: 目的：提供用于诊断或检测PSV（花生特技病毒）的探针，以尽量减少作物病毒性疾病引起的损伤，并提高产量。 构成：用于诊断或检测PSV的寡核苷酸探针组含有选自序列号1025-1034,1045-1049和2988的寡核苷酸及其互补寡核苷酸的一种或多种寡核苷酸。 用于诊断或检测PSV的方法包括：从样品中分离RNA的步骤; 合成cDNA的步骤; 将cDNA与探针组杂交的步骤; 以及检测cDNA和探针组杂交的步骤。 （AA）Cow豆; （BB）主持人：cow豆; （CC）芸豆; （DD）主机：芸豆

公开(公告)号：KR1020130054494A

公开(公告)日：2013-05-27

申请号：KR1020110117752

申请日：2011-11-11

Applicant: 한국생명공학연구원 ,  대한민국(농촌진흥청장)

Inventor： 문제선 ,  이수헌 ,  김성욱 ,  임승모 ,  남문 ,  김정선 ,  최홍수 ,  이준성 ,  김정수

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/04

CPC classification number: C12Q1/701 ,  C12Q1/04 ,  C12Q1/6837

Abstract: PURPOSE: A probe set for diagnosing or detecting BBWV2(Broad bean wilt virus2) is provided to minimize damage due to viral diseases in crops and to increase product yield. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting BBWV2 contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 1143-1152 and 1158-1164 and complementary oligonucleotides thereof. A method for diagnosing or detecting BBWV2 comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA using RNA as a template; a step of hybridizing the probe set with cDNA; and a step of detecting hybridization.

Abstract translation: 目的：提供用于诊断或检测BBWV2（蚕豆枯萎病毒2）的探针，以尽量减少作物病毒性疾病造成的损伤，并提高产品产量。 构成：用于诊断或检测BBWV2的寡核苷酸探针组含有选自序列号1143-1152和1158-1164的寡核苷酸及其互补寡核苷酸的一种或多种寡核苷酸。 一种用于诊断或检测BBWV2的方法包括：从样品中分离RNA的步骤; 使用RNA作为模板合成cDNA的步骤; 探针组与cDNA杂交的步骤; 和检测杂交的步骤。

公开(公告)号：KR1020130054484A

公开(公告)日：2013-05-27

申请号：KR1020110117611

申请日：2011-11-11

Applicant: 한국생명공학연구원 ,  대한민국(농촌진흥청장)

Inventor： 문제선 ,  이수헌 ,  김성욱 ,  임승모 ,  남문 ,  김정선 ,  최홍수 ,  이준성 ,  김정수

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/04

CPC classification number: C12Q1/701 ,  C12Q1/04 ,  C12Q1/6837

Abstract: PURPOSE: A probe set for diagnosing Genus Carmovirus is provided to minimize viral diseases in crops and to enhance product yields. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting Genus Carmovirus contains one or more oligonucleotides selected from a group consisting of oligonucleotides of sequence numbers 674, 682-685, 691-692, 730-734, 746-748, 1452-1458, 1665-1672, 2371, and 2745-2754, and complementary oligonucleotides thereof. A method for diagnosing or detecting Genus Carmovirus comprises: a step of isolating RNA from a sample which is suspected to have Genus Carmovirus infection; a step of synthesizing cDNA; a step of hybridizing the cDNA and the probe set; and a step of detecting hybridization. [Reference numerals] (AA) Jangyeobkong; (BB) Host: Jangyeobkong

Abstract translation: 目的：提供用于诊断卡门诺病毒属的探针，以尽量减少作物中的病毒性疾病，并提高产品产量。 构成：用于诊断或检测属Carmovirus属的寡核苷酸探针组含有一种或多种选自序列号674,682-685,691-692,730-734,746-748,1452-1458,1665的寡核苷酸的寡核苷酸 -1672,2731和2745-2754及其互补寡核苷酸。 用于诊断或检测属Carmovirus的方法包括：从怀疑具有Carmovirus类病毒感染的样品中分离RNA的步骤; 合成cDNA的步骤; cDNA和探针组杂交的步骤; 和检测杂交的步骤。 （附图标记）（AA）Jangyeobkong; （BB）主持人：Jangyeobkong

公开(公告)号：KR1020080013415A

公开(公告)日：2008-02-13

申请号：KR1020060074894

申请日：2006-08-08

Applicant: 대한민국(농촌진흥청장)

Inventor： 이수헌 ,  신용길 ,  문제선

IPC: C12Q1/68

CPC classification number: C12Q1/6844

Abstract: Specific primers for detection of tabacco streak virus are provided to improve specificity of detection by performing the species specific detection through RT-PCR(reverse transcription polymerase chain reaction), and enhance limit of detection by resolving non-specific reactions of serological diagnosis. Specific primers for detection of tabacco streak virus used for RT-PCR comprise at least one nucleic acid fragment selected from the nucleotide sequence of SEQ ID NOs:1, 2, 5 and 7, complementary sequences thereof and mutated sequences which are prepared by deletion, addition or substitution of partial bases, wherein the primer is a combination of a nucleic acid fragment of SEQ ID NO:1 and a nucleic acid fragment of SEQ ID NO:7, or a combination of a nucleic acid fragment of SEQ ID NO:2 and a nucleic acid fragment of SEQ ID NO:5.

Abstract translation: 提供用于检测烟草条纹病毒的特异性引物，通过RT-PCR（逆转录聚合酶链式反应）进行物种特异性检测，提高检测特异性，通过解决血清学诊断的非特异性反应来增强检测限。 用于检测用于RT-PCR的烟草条纹病毒的特异性引物包含至少一个选自SEQ ID NO：1,2,5和7的核苷酸序列的核酸片段，其互补序列和突变序列，其通过缺失， 部分碱基的添加或取代，其中所述引物是SEQ ID NO：1的核酸片段和SEQ ID NO：7的核酸片段的组合，或SEQ ID NO：2的核酸片段的组合 和SEQ ID NO：5的核酸片段。