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51.发明公开자일리톨 탈수소효소가 불활화되고 아라비노스 환원효소의 활성이 억제된 캔디다 트로피칼리스 변이주와 그것을 이용한 자일리톨 고수율 생산방법 无效甘草酸脱氢酶抑制剂和阿拉伯糖苷抑制剂甘草酸热菌的制备方法，使用其制备XYLITOL的高产率和水解产物的方法和生产的XYLILTOLY
公开(公告)号：KR1020090013617A
公开(公告)日：2009-02-05
申请号：KR1020070077889
申请日：2007-08-02
CPC classification number: C12P7/18 , C12N1/14 , C12N9/0006 , C12N15/80 , C12Y101/01009
Abstract: A method for producing xylitol by using the mutated Candida tropicalis strain is provided to simplifying the production process by eliminating the additional process for maintaining the dissolved oxygen concentration in culture medium to be very low, and inhibit production of by-products such as ethanol and glycerol generated by cultivating the strain under low dissolved oxygen condition. The method for producing xylitol by using the mutated Candida tropicalis strain BS-xdh1(KCTC 11137BP) in which xylitol dehydrogenase is inactivated and arabinose reductase activity is suppressed comprises the steps of: preparing the xylitol production medium containing the biomass hydrolysate as a substrate; inoculating the Candida tropicalis strain BS-xdh1(KCTC 11137BP) into the production medium; and cultivating the strain with sufficient agitation without the separate process of controlling the dissolved oxygen concentration to the low concentration of 0.5 % or 2 %.
Abstract translation: 提供了通过使用突变的热带假丝酵母菌株生产木糖醇的方法，通过消除维持培养基中溶解氧浓度的额外方法来简化生产过程，并抑制副产物如乙醇和甘油的产生 通过在低溶解氧条件下培养菌株产生。 通过使用其中木糖醇脱氢酶失活的热带假丝酵母菌株BS-xdh1（KCTC 11137BP）和阿拉伯糖还原酶活性被抑制的生产木糖醇的方法包括以下步骤：制备含有生物质水解产物作为底物的木糖醇生产培养基; 将热带假丝酵母菌株BS-xdh1（KCTC 11137BP）接种到生产培养基中; 并且在没有将溶解氧浓度控制到0.5％或2％的低浓度的单独过程的情况下充分搅拌培养菌株。
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