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    HEAT-INDUCIBLE N-DEGRON MODULE
    53.
    发明申请
    HEAT-INDUCIBLE N-DEGRON MODULE 审中-公开
    热诱导型N-DEGRON模块

    公开(公告)号：WO1995021267A1

    公开(公告)日：1995-08-10

    申请号：PCT/US1995001268

    申请日：1995-01-30

    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

    Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  VARSHAVSKY, Alexander ,  DOHMEN, Jurgen ,  JOHNSTON, Jennifer ,  WU, Peipei

    IPC: C12Q01/68

    CPC classification number: C12N9/0028 ,  C07K14/00 ,  C07K14/001 ,  C07K14/395 ,  C07K2319/00 ,  C07K2319/42 ,  C07K2319/61 ,  C07K2319/95 ,  C12N9/003 ,  C12N9/12 ,  C12N15/11 ,  C12N15/62

    Abstract: The N-degron is an intracellular degradation signal whose essential determinant is a specific, destabilizing, N-terminal amino acid residue. A set of N-degrons containing different destabilizing residues is manifested as the N-end rule, which relates the in vivo half-life of a protein to the identity of its N-terminal amino acid residue. Disclosed herein is a heat-inducible N-degron module. A heat-inducible N-degron module is a protein or peptide bearing a destabilizing N-terminal amino acid residue which becomes a substrate of the N-end rule pathway only at a temperature high enough to result in at least partial unfolding of the protein. At this elevated (nonpermissive) temperature, the heat-inducible N-degron module (and any protein or peptide attached at its C-terminus) is rapidly degraded in a cell in which the N-end rule pathway is operative. Also disclosed are DNA and protein fusion constructs, methods for screening for additional heat-inducible N-degron modules and methods for using the disclosed heat-inducible N-degron modules.

    Abstract translation: N-degron是细胞内降解信号，其主要决定簇是特异的，不稳定的N-末端氨基酸残基。 含有不同去稳定残基的一组N-解码子表现为N端规则，其将蛋白质的体内半衰期与其N-末端氨基酸残基的身份相关联。 本文公开了一种热诱导型N-离子解聚模块。 热诱导型N-解体模块是具有不稳定的N-末端氨基酸残基的蛋白质或肽，其仅在足以导致蛋白质的至少部分展开的温度下成为N端规则通路的底物。 在这种升高的（非允许的）温度下，在N端规则通路可操作的细胞中，热诱导型N-脱落元件模块（以及在其C-末端附着的任何蛋白质或肽）被快速降解。 还公开了DNA和蛋白质融合构建体，用于筛选额外的热诱导性N-脱落模块的方法以及使用所公开的热诱导型N-脱落伞模块的方法。

    HIGH PERFORMANCE THERMOELECTRIC MATERIALS AND METHODS OF PREPARATION
    54.
    发明申请
    HIGH PERFORMANCE THERMOELECTRIC MATERIALS AND METHODS OF PREPARATION 审中-公开
    高性能热电材料及其制备方法

    公开(公告)号：WO1995004377A1

    公开(公告)日：1995-02-09

    申请号：PCT/US1994008452

    申请日：1994-07-28

    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

    Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  FLEURIAL, Jean-Pierre ,  CAILLAT, Thierry, F. ,  BORSHCHEVSKY, Alexander

    IPC: H01L35/18

    CPC classification number: H01L35/22 ,  H01L35/18 ,  H01L35/32 ,  Y10S257/93

    Abstract: Transition metals (T) of Group VIII (Co, Rh and Ir) have been prepared as semiconductor alloys with Sb having the general formula TSb3. The skutterudite-type crystal lattice structure of these semiconductor alloys and their enhanced thermoelectric properties results in semiconductor materials which may be used in the fabrication of thermoelectric elements to substantially improve the efficiency of the resulting thermoelectric device. Semiconductor alloys having the desired skutterudite-type crystal lattice structure may be prepared in accordance with the present invention by using vertical gradient freeze techniques, liquid-solid phase sintering techniques, low temperature powder sintering and/or hot-pressing. Measurements of electrical and thermal transport properties of selected semiconductor materials prepared in accordance with the present invention, demonstrated high Hall mobilities (up to 8000 cm .V .s ), good Seebeck coefficients (up to 400 mu VK between 300C and 700C), and low thermal conductivities (as low as 15 m &cir& _W/cmK). Optimizing the transport properties of semiconductor materials prepared from elemental mixtures Co, Rh, Ir and Sb resulted in a two fold increase in the thermoelectric figure of merit (ZT) at temperatures as high as 400C for thermoelectric elements fabricated from such semiconductor materials.

    Abstract translation: 已经制备了第VIII族（Co，Rh和Ir）的过渡金属（T）作为具有通式为TSb3的Sb的半导体合金。 这些半导体合金的方钴矿型晶格结构及其增强的热电性能导致可用于制造热电元件的半导体材料，以显着提高所得热电装置的效率。 具有期望的方钴矿型晶格结构的半导体合金可以通过使用垂直梯度冷冻技术，液相 - 固相烧结技术，低温粉末烧结和/或热压法，根据本发明制备。 根据本发明制备的所选择的半导体材料的电和热传输性能的测量显示了高的霍尔迁移率（高达8000cm 2 .V -1 -1），良好的塞贝克系数 在300℃至700℃之间的400℃VK -1）和低热导率（低至15mΩ/ w / cmK）。 从元素混合物Co，Rh，Ir和Sb制备的半导体材料的传输性能优化导致在由这种半导体材料制造的热电元件的高达400℃的温度下，热电性能（ZT）增加了两倍。

    REACTION CELL FOR PROTEIN SEQUENCER AND THE LIKE
    55.
    发明申请
    REACTION CELL FOR PROTEIN SEQUENCER AND THE LIKE 审中-公开
    蛋白质序列的反应细胞和类似物

    公开(公告)号：WO1995002455A1

    公开(公告)日：1995-01-26

    申请号：PCT/US1994007523

    申请日：1994-07-05

    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

    Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  STOLOWITZ, Mark, L.

    IPC: B01L03/00

    CPC classification number: B01L3/508 ,  B01L3/502 ,  B01L2300/0809 ,  B01L2300/0877 ,  G01N1/28

    Abstract: The present invention comprises a reaction cell for a sequencer and the like which includes a sample carrier assembly (60) having a flexible, resilient spacer (74) with a hole therein for retaining the sample on a membrane (not shown), a pair of alumina cells (70 and 80) which bracket the spacer (74), one or more pads (64) or spacers to retain the assembly in a tight fitting relation and a cap (84) to secure the assembly together. The reagents are input and withdrawn from opposite edges of the sample membrane. The present invention has a minimal dead volume as a result of the spacer arrangement. It also permits benchtop sample loading due to the separation of the assembly into a carrier (60) and base housing (20, 28 and 50). The fluid lines (26a and 26b) are self-sealing to the reaction cell.

    Abstract translation: 本发明包括用于定序器等的反应池，其包括具有柔性弹性隔离物（74）的样品载体组件（60），其中具有用于将样品保持在膜（未示出）上的孔，一对 支撑间隔物（74）的氧化铝电池（70和80），一个或多个衬垫（64）或间隔件以将组件保持紧密配合关系和帽（84）以将组件固定在一起。 试剂从样品膜的相对边缘输入并取出。 本发明由于间隔件的结构而具有最小的死体积。 由于组件分离成载体（60）和基座壳体（20,28和50），它还允许台式样品加载。 流体管线（26a和26b）对反应池自密封。

    TEMPLATE-DIRECTED RING-CLOSING METATHESIS AND RING-OPENING METATHESIS POLYMERIZATION OF FUNCTIONALIZED DIENES
    59.
    发明申请
    TEMPLATE-DIRECTED RING-CLOSING METATHESIS AND RING-OPENING METATHESIS POLYMERIZATION OF FUNCTIONALIZED DIENES 审中-公开
    模板方向闭环特征和开环功能化二氧化碳的分解聚合

    公开(公告)号：WO1998030557A1

    公开(公告)日：1998-07-16

    申请号：PCT/US1997023929

    申请日：1997-12-19

    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

    Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  GRUBBS, Robert, H. ,  MARSELLA, Michael, J. ,  MAYNARD, Heather, D.

    IPC: C07D323/00

    CPC classification number: C07D323/00 ,  C08G65/329

    Abstract: Functionalized cyclic olefins and methods for making the same are disclosed. Methods include template-directed ring-closing metathesis ("RCM") of functionalized acyclic dienes and template-directed depolymerization of functionalized polymers possessing regularly spaced sties of unsaturation. Although the template species may be any anion, cation, or dipolar compound, cationic species, especially alkali metals, are preferred. Functionalized polymers with regularly spaced sites of unsaturation and methods for making the same are also disclosed. One method for synthesizing these polymers is by ring-opening metathesis polymerization ("ROMP") of functionalized cyclic olefins. The metathesis catalysts for both RCM and ROMP reaction are ruthenium or osmium carbene complex catalysts of formula (I) where M is Os or Ru; R and R each may be hydrogen, C2-C20 alkenyl, C2-C20 alkynyl, C1-C20 alkyl, aryl, C1-C20 carboxylate, C1-C20 alkoxy, C2-C20 alkenyloxy, C2-C20 alkynyloxy, aryloxy, C2-C20 alkoxycarbonyl, C1-C20 alkylthio, C1-C20 alkylsulfonyl or C1-C20 alkylsulfinyl; X and X may be any anionic ligand; and L and L may be any neutral electron donor.

    Abstract translation: 公开了官能化环烯烃及其制备方法。 方法包括官能化无环二烯的模板定向闭环复分解（“RCM”）和具有规则间隔不饱和度的官能化聚合物的模板定向解聚。 尽管模板物质可以是任何阴离子，阳离子或偶极化合物，但优选阳离子物质，特别是碱金属。 还公开了具有规则间隔的不饱和位置的官能化聚合物及其制备方法。 合成这些聚合物的一种方法是通过官能化环烯烃的开环易位聚合（“ROMP”）。 用于RCM和ROMP反应的复分解催化剂是式（I）的钌或咔卡因络合物催化剂，其中M是Os或Ru; R和R 1各自可以是氢，C 2 -C 20烯基，C 2 -C 20炔基，C 1 -C 20烷基，芳基，C 1 -C 20羧酸酯，C 1 -C 20烷氧基，C 2 -C 20链烯氧基，C 2 -C 20炔氧基，芳氧基， C 2 -C 20烷氧基羰基，C 1 -C 20烷硫基，C 1 -C 20烷基磺酰基或C 1 -C 20烷基亚磺酰基; X和X 1可以是任何阴离子配体; L和L 1可以是任何中性电子给体。

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