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    DIGITAL BRIDGE PCR
    51.
    发明申请
    DIGITAL BRIDGE PCR 审中-公开
    数字桥接PCR

    公开(公告)号：US20140378333A1

    公开(公告)日：2014-12-25

    申请号：US14344739

    申请日：2012-09-13

    Applicant: David R. Walt ,  David C. Duffy

    Inventor： David R. Walt ,  David C. Duffy

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/682 ,  C12Q1/6876 ,  C12Q2563/107 ,  C12Q2600/16 ,  C12Q2600/178

    Abstract: The present disclosure, among other things, provides methodologies for quantifying targets of interest in samples by 1) capturing single target entities on individual solid phase particles in a manner that permits that those particles that contain captured targets to be optically distinguished from those that do not, and 2) optically analyzing the particles so that those with captured target entities are “counted”. In some embodiments, provided methods and compositions in the present application comprise a population of particles including one or more sub-populations distinguishable from one another.

    Abstract translation: 本公开尤其提供了通过以下方式量化样品中感兴趣的目标的方法：1）以允许将包含捕获的靶的那些颗粒与不相似的那些颗粒光学地区分开的方式捕获单个固相颗粒上的单个目标实体 ，和2）光学分析颗粒，使得具有捕获的目标实体的那些被“计数”。 在一些实施方案中，本申请中提供的方法和组合物包含一群粒子，包括可彼此区分的一个或多个亚种群。

    ULTRA-SENSITIVE DETECTION OF MOLECULES OR PARTICLES USING BEADS OR OTHER CAPTURE OBJECTS
    54.
    发明申请
    ULTRA-SENSITIVE DETECTION OF MOLECULES OR PARTICLES USING BEADS OR OTHER CAPTURE OBJECTS 有权
    使用珠子或其他捕获物体对分子或颗粒进行超敏感检测

    公开(公告)号：US20110212848A1

    公开(公告)日：2011-09-01

    申请号：US12731130

    申请日：2010-03-24

    Applicant: David C. Duffy ,  David M. Rissin ,  David R. Walt ,  David Fournier ,  Cheuk Kan

    Inventor： David C. Duffy ,  David M. Rissin ,  David R. Walt ,  David Fournier ,  Cheuk Kan

    IPC: C40B30/04 ,  C40B40/04

    CPC classification number: G01N33/54393 ,  G01N33/54306 ,  G01N33/54313

    Abstract: The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.

    Abstract translation: 本发明涉及用于检测流体样品中的分析物分子或颗粒的系统和方法，并且在某些情况下，确定流体样品中分子或颗粒的浓度的量度。 本发明的方法可以包括相对于多个捕获物体固定多个分析物分子或颗粒。 多个捕获对象的至少一部分可以在空间上分离成多个位置。 可以至少部分地确定包含相对于捕获对象固定的分析物分子的反应容器的数量，来测量流体样品中分析物分子的浓度。 在一些情况下，该测定可另外包括包括结合配体，前体标记试剂和/或酶组分的步骤。

    Self-encoding sensor with microspheres
    57.
    发明授权
    Self-encoding sensor with microspheres 失效
    具有微球的自编码传感器

    公开(公告)号：US07754498B2

    公开(公告)日：2010-07-13

    申请号：US11040504

    申请日：2005-01-21

    Applicant: David R. Walt ,  Todd A. Dickinson

    Inventor： David R. Walt ,  Todd A. Dickinson

    IPC: G01N33/543 ,  G01N21/00 ,  G01N21/75

    CPC classification number: C12Q1/682 ,  B01J2219/00317 ,  B01J2219/0045 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00524 ,  B01J2219/00585 ,  B01J2219/00648 ,  B01J2219/00659 ,  C12Q1/6834 ,  C40B60/14 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7703 ,  G01N33/0031 ,  G01N33/54313 ,  G01N2201/0846 ,  Y10T436/101666 ,  Y10T436/109163

    Abstract: A microsphere-based analytic chemistry system is disclosed in which self-encoding microspheres having distinct characteristic optical response signatures to specific target analytes may be mixed together while the ability is retained to identify the sensor type and location of each sensor in a random dispersion of large numbers of such sensors in a sensor array using an optically interrogatable encoding scheme. An optical fiber bundle sensor is also disclosed in which individual microsphere sensors are disposed in microwells at a distal end of the fiber bundle and are optically coupled to discrete fibers or groups of fibers within the bundle. The identities of the individual sensors in the array are self-encoded by exposing the array to a reference analyte while illuminating the array with excitation light energy. A single sensor array may carry thousands of discrete sensing elements whose combined signal provides for substantial improvements in sensor detection limits, response times and signal-to-noise ratios.

    Abstract translation: 公开了一种基于微球的分析化学系统，其中具有针对特定目标分析物的不同特征光学响应特征的自编码微球可以混合在一起，同时保留能力以识别大尺度随机色散中的每个传感器的传感器类型和位置 使用光学可询问编码方案的传感器阵列中的这种传感器的数量。 还公开了一种光纤束传感器，其中单个微球传感器设置在纤维束的远端处的微孔中，并且光束耦合到束内的离散纤维或纤维组。 阵列中各个传感器的身份通过将阵列暴露于参考分析物而进行自编​​码，同时用激发光能照射阵列。 单个传感器阵列可携带数千个离散感测元件，其组合信号提供传感器检测限，响应时间和信噪比方面的实质性改进。

    METHOD AND SYSTEM OF ARRAY IMAGING
    58.
    发明申请
    METHOD AND SYSTEM OF ARRAY IMAGING 审中-公开
    阵列成像的方法与系统

    公开(公告)号：US20100032547A1

    公开(公告)日：2010-02-11

    申请号：US12567618

    申请日：2009-09-25

    Applicant: David R. Walt ,  Sandra Bencic-Nagale

    Inventor： David R. Walt ,  Sandra Bencic-Nagale

    IPC: G01N21/00 ,  H01L31/0232

    CPC classification number: G01J3/2803 ,  G01J3/02 ,  G01J3/0213 ,  G01J3/0229 ,  G01J3/10 ,  G01J3/4406 ,  G01J2001/0276 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6441 ,  G01N2021/6484 ,  G01N2201/08 ,  G01N2201/088

    Abstract: The present invention relates to a method and system of array imaging that extends or maximizes the longevity of the sensor array by minimizing the effects of photobleaching. The imaging system has a light source, a variable exposure aperture, and a variable filter system. The system extends the longevity of sensors by (1) using the variable exposure aperture to selectively expose sections of the sensor array containing representative numbers of each type of sensor, and/or (2) using the variable filter system to control the intensity of the excitation light, providing only the intensity required to induce the appropriate excitation and increasing that intensity over time as necessary to counteract the effects of photobleaching.

    Abstract translation: 本发明涉及阵列成像的方法和系统，该方法和系统通过最小化光漂白的影响来延长或最大化传感器阵列的寿命。 成像系统具有光源，可变曝光孔径和可变过滤器系统。 该系统通过（1）使用可变曝光孔径来延长传感器的寿命，以选择性地暴露包含每种类型的传感器的代表数的传感器阵列的部分，和/或（2）使用可变过滤器系统来控制传感器的强度 激发光，仅提供诱导适当激发所需的强度，并随时间增加强度以抵消光漂白的影响。

    Cross-reactive sensors
    59.
    发明申请
    Cross-reactive sensors 审中-公开
    交叉反应传感器

    公开(公告)号：US20100028923A1

    公开(公告)日：2010-02-04

    申请号：US11820882

    申请日：2007-06-21

    Applicant: David R. Walt ,  Caroline L. Schauer ,  Frank J. Steemers

    Inventor： David R. Walt ,  Caroline L. Schauer ,  Frank J. Steemers

    IPC: C12Q1/44 ,  C40B40/10

    CPC classification number: C12Q1/44 ,  C12Q1/37

    Abstract: The present invention provides a novel cross-reactive sensor system utilizing cross-reactive recognition elements. In the inventive system, each of said one or more cross-reactive recognition elements is capable of interacting with more than one species of liquid analyte of interest, whereby each of said one or more cross-reactive recognition elements reacts in a different manner with each of said one or more species of liquid analytes of interest to produce a detectable agent of each analyte of interest, whereby said detectable agent is analyzed and the information is processed for data acquisition and interpretation. In certain preferred embodiments, the detectable agent and/or change is detected directly, while in certain other preferred embodiments, the detectable agent and/or change is detected with the help of a transducing agent capable of relaying information about each detectable agent generated for each of said species of liquid analyte of interest, whereby said information is processed for data acquisition and interpretation. The present invention also provides method for the analysis of analytes comprising contacting one or more analytes with the inventive system described above.

    Abstract translation: 本发明提供了一种利用交叉反应识别元件的新型交叉反应传感器系统。 在本发明的系统中，所述一个或多个交叉反应性识别元件中的每一个能够与多于一种的感兴趣的液体分析物相互作用，由此所述一个或多个交叉反应性识别元件中的每一个以不同的方式与每个 的所述一种或多种感兴趣的液体分析物种类以产生感兴趣的每种分析物的可检测试剂，由此分析所述可检测试剂，并且对所述信息进行处理以进行数据采集和解释。 在某些优选的实施方案中，直接检测可检测的试剂和/或变化，而在某些其它优选的实施方案中，可检测的试剂和/或变化是借助于能够中继关于每个可产生的每种可检测试剂的信息的转导剂 的所述物质的感兴趣的液体分析物，由此所述信息被处理用于数据采集和解释。 本发明还提供了用于分析分析物的方法，包括使一种或多种分析物与上述本发明的系统接触。

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